An optimized protocol of a human-to-cattle interspecies somatic cell nuclear transfer

Received 17 October 2003; received in revised form 9 March 2004; accepted 9 March 2004.

Human somatic cells were transferred into cattle enucleated oocytes, and a prospective, randomized study was designed to optimize donor cell preparation, fusion medium, and culture method. As a result, improved development of interspecies embryos was achieved by employing serum-starved cord fibroblasts, with Ca2+/Mg2+-free fusion medium and a serum-free medium being used for bovine embryos.

a School of Agricultural Biotechnology, Seoul National University, Seoul, Korea

c College of Veterinary Medicine, Seoul National University, Seoul, Korea

d Medical Research Center, College of Medicine,d Seoul National University, Seoul, Korea

b Stem Cell Research Center, Seoul, Korea

Reprint requests: Jeong M. Lim, D.V.M., Ph.D., School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea (FAX: 822-874-2555

Supported by a graduate fellowship provided by the Korean Ministry of Education through the BK 21 project, Seoul, and by a grant from Stem Cell Research Center (SC11011).

PII: S0015-0282(04)01291-9

doi:10.1016/j.fertnstert.2004.03.052

47 of 58