Fertility and Sterility
Volume 91, Issue 5, Supplement , Pages 2037-2042, May 2009

Effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent mouse embryo development

  • Byung Chul Jee, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, South Korea
    • Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada
    • ,
  • Hai-Ying Chen, B.Sc.

      Affiliations

    • Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada
    • ,
  • Ri-Cheng Chian, Ph.D.

      Affiliations

    • Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada
    • Corresponding Author InformationReprint requests: Ri-Cheng Chian, Ph.D., Department of Obstetrics and Gynecology, Royal Victoria Hospital, Women's Pavilion F3-46, 687 Pine Avenue West, Montreal, Quebec H31 1A1, Canada (FAX: 514-843-1662).

Received 13 February 2008; received in revised form 12 March 2008; accepted 13 March 2008. published online 12 June 2008.

Objective

To investigate the effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent embryo development.

Design

Animal study.

Setting

University laboratory.

Animal(s)

CD-1 mice.

Intervention(s)

Immature oocytes were arrested meiotically in medium containing a specific phosphodiesterase type 3 inhibitor, cilostamide (1 μmol/L, 5 μmol/L, and 10 μmol/L), for 6 and 24 hours of prematuration culture, and then matured in vitro in inhibitor-free medium.

Main Outcome Measure(s)

Oocyte maturation, fertilization, and early embryonic development in vitro.

Result(s)

After 6 and 24 hours of prematuration culture with 1 μmol/L, 5 μmol/L, and 10 μmol/L cilostamide, there were no differences in maturation, fertilization, and early embryonic development compared with non-arrested controls.

Conclusion(s)

Prematuration culture with cilostamide, for either 6 or 24 hours, may not be beneficial for subsequent embryonic development of immature mouse oocytes.

Key Words: In vitro maturation, immature oocytes, phosphodiesterase inhibitor, meiotic arrest, prematuration culture

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 B.C.J. has nothing to disclose. H.Y.C. has nothing to disclose. R.-C.C. has nothing to disclose.

PII: S0015-0282(08)00708-5

doi:10.1016/j.fertnstert.2008.03.041

Fertility and Sterility
Volume 91, Issue 5, Supplement , Pages 2037-2042, May 2009

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