Effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent mouse embryo development
Received 13 February 2008; received in revised form 12 March 2008; accepted 13 March 2008. published online 12 June 2008.
Objective
To investigate the effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent embryo development.
Design
Animal study.
Setting
University laboratory.
Animal(s)
CD-1 mice.
Intervention(s)
Immature oocytes were arrested meiotically in medium containing a specific phosphodiesterase type 3 inhibitor, cilostamide (1 μmol/L, 5 μmol/L, and 10 μmol/L), for 6 and 24 hours of prematuration culture, and then matured in vitro in inhibitor-free medium.
Main Outcome Measure(s)
Oocyte maturation, fertilization, and early embryonic development in vitro.
Result(s)
After 6 and 24 hours of prematuration culture with 1 μmol/L, 5 μmol/L, and 10 μmol/L cilostamide, there were no differences in maturation, fertilization, and early embryonic development compared with non-arrested controls.
Conclusion(s)
Prematuration culture with cilostamide, for either 6 or 24 hours, may not be beneficial for subsequent embryonic development of immature mouse oocytes.
aDepartment of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, South Korea
bDepartment of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada
Reprint requests: Ri-Cheng Chian, Ph.D., Department of Obstetrics and Gynecology, Royal Victoria Hospital, Women's Pavilion F3-46, 687 Pine Avenue West, Montreal, Quebec H31 1A1, Canada (FAX: 514-843-1662).
B.C.J. has nothing to disclose. H.Y.C. has nothing to disclose. R.-C.C. has nothing to disclose.
ABSTRACT