Human papillomavirus found in sperm head of young adult males affects the progressive motility

Foresta, Carlo; Garolla, Andrea; Zuccarello, Daniela; Pizzol, Damiano; Moretti, Afra; Barzon, Luisa; Palù, Giorgio

doi:10.1016/j.fertnstert.2008.10.050

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Volume 93, Issue 3 , Pages 802-806, February 2010

Human papillomavirus found in sperm head of young adult males affects the progressive motility

Carlo Foresta, M.D.
- Department of Histology, Microbiology and Medical Biotechnologies, Section of Clinical Pathology and Centre for Male Gamete Cryopreservation, University of Padova, Italy
- Reprint requests: Prof. Carlo Foresta, University of Padova, Department of Histology, Microbiology and Medical Biotechnologies, Section of Clinical Pathology and Centre for Male Gamete Cryopreservation, Via Gabelli 63 - 35121 Padova, Italy (TEL: +39 049 8218517; FAX: +39 049 8218520).
Andrea Garolla, M.D.
- Department of Histology, Microbiology and Medical Biotechnologies, Section of Clinical Pathology and Centre for Male Gamete Cryopreservation, University of Padova, Italy
Daniela Zuccarello, M.D.
- Department of Histology, Microbiology and Medical Biotechnologies, Section of Clinical Pathology and Centre for Male Gamete Cryopreservation, University of Padova, Italy
Damiano Pizzol, M.S.
- Department of Histology, Microbiology and Medical Biotechnologies, Section of Clinical Pathology and Centre for Male Gamete Cryopreservation, University of Padova, Italy
Afra Moretti, B.Sc.
- Department of Histology, Microbiology and Medical Biotechnologies, Section of Clinical Pathology and Centre for Male Gamete Cryopreservation, University of Padova, Italy
Luisa Barzon, M.D.
- Department of Histology, Microbiology and Medical Biotechnologies, Section of Microbiology and Medical Biotechnologies, University of Padova, Italy
Giorgio Palù, M.D.
- Department of Histology, Microbiology and Medical Biotechnologies, Section of Microbiology and Medical Biotechnologies, University of Padova, Italy

Received 18 August 2008; received in revised form 14 October 2008; accepted 16 October 2008. published online 18 December 2008.

Objective

To evaluate the prevalence of human papillomavirus (HPV) sperm infection and its correlation with sperm parameters in a cohort of young adult males.

Design

Cross-sectional clinical study.

Setting

Andrology and Microbiology sections at a university hospital.

Patient(s)

A cohort of 200 young adult male volunteers (18 years old), 100 with previous sexual intercourse and 100 without previous sexual intercourse.

Main Outcome Measure(s)

Seminal parameters, sperm culture for HPV and fluorescence in situ hybridization (FISH) analysis for HPV detection in the sperm head. Statistical analysis was performed with a two-tailed Student's t-test.

Result(s)

Results of HPV investigation were compared to sperm parameters and results of FISH analysis. HPV infection was present in sperm cells of 10 subjects among those 100 young adults who already had unprotected intercourse and its presence was associated with reduced sperm motility. Furthermore, infected samples showed that about 25% of sperm had an HPV DNA positivity at the head site, but it is unclear whether it was integrated in the nucleus or not.

Conclusion(s)

This is the first report estimating the percentage of HPV-positive sperm in infected subjects and the association between HPV infection and sperm motility.

Key Words: asthenozoospermia, HPV, young adults, nuclear integration, spermatozoa, sperm motility

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C.F. has nothing to disclose. A.G. has nothing to disclose. D.Z. has nothing to disclose. D.P. has nothing to disclose. A.M. has nothing to disclose. L.B. has nothing to disclose. G.P. has nothing to disclose.

PII: S0015-0282(08)04408-7

doi:10.1016/j.fertnstert.2008.10.050

« Previous Next »Fertility and Sterility
Volume 93, Issue 3 , Pages 802-806, February 2010

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