Immunohistochemical analysis of the role of angiogenic status in the vasculature of peritoneal endometriosis

Matsuzaki, Sachiko; Canis, Michel; Murakami, Takashi; Dechelotte, Pierre; Bruhat, Maurice A; Okamura, Kunihiro

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Volume 76, Issue 4 , Pages 712-716, October 2001

Immunohistochemical analysis of the role of angiogenic status in the vasculature of peritoneal endometriosis

Sachiko Matsuzaki, M.D.
- Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan
- Department of Gynecology, Polyclinique de l’Hôtel-Dieu, Clermont-Ferrand, France
- Reprint requests: Sachiko Matsuzaki, M.D., Department of Gynecology, Polyclinique de l’Hotel-Dieu, 13, boulevard Charles-De-Gaulle, 63003 Clermont-Ferrand Cedex 1, France. (FAX: 33-4-73-93-17-06)
Michel Canis, M.D.
- Department of Gynecology, Polyclinique de l’Hôtel-Dieu, Clermont-Ferrand, France
Takashi Murakami, M.D.
- Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan
Pierre Dechelotte, M.D., Ph.D.,
- Department of Pathology, Polyclinique de l’Hôtel-Dieu, Clermont-Ferrand, France
Maurice A Bruhat, M.D.
- Department of Gynecology, Polyclinique de l’Hôtel-Dieu, Clermont-Ferrand, France
Kunihiro Okamura, M.D.
- Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan

Received 12 February 2001; received in revised form 19 April 2001; accepted 19 April 2001.

Abstract

Objective: To investigate the angiogenic status of the vasculature in peritoneal endometriosis based on macroscopic appearance.

Design: Prospective study.

Setting: University hospital.

Patient(s): Patients with peritoneal endometriosis.

Intervention(s): During laparoscopy, 25 samples of predominantly red peritoneal lesions and 27 samples of predominantly black peritoneal lesions were collected from a total of 31 patients with endometriosis. Eutopic endometrium from 25 patients with endometriosis was collected by curettage during laparoscopy or just after surgery.

Main Outcome Measure(s): A proliferating endothelial cell index (PCI) was determined by calculating the percentage of microvessels that contained proliferating endothelial cells. A vessel maturation index (VMI) was determined by calculating the percentage of microvessels showing colocalization of CD34- and alpha-SMA-positive staining.

Result(s): Peritoneal endometriotic tissues had extremely low or null PCI. The VMI of red peritoneal lesions was significantly lower than that of black ones. Vessel maturation index of red lesions was higher that that of proliferative eutopic endometrium and similar to that of secretory eutopic endometrium.

Conclusion(s): Compared with the case of black peritoneal lesions, red lesions had a much higher fraction of immature vessels.

Keywords: Angiogenesis, microvessel, pericyte, peritoneal endometriosis