Prediction of chromosome misalignment among in vitro matured human oocytes by spindle imaging with the PolScope

Abstract 

Objective: To examine whether spindle morphologic features imaged with the LC-PolScope (Cambridge Research and Instrumentation, Woburn, MA) in living human oocytes matured in vitro can be used to predict chromosome configuration and select oocytes with normal chromosomes.

Design: Morphological study.

Setting: Academic IVF clinic.

Patient(s): Women undergoing oocyte retrieval for ICSI treatment.

Intervention(s): Oocytes were examined after in vitro maturation.

Main Outcome Measure(s): The study examined meiotic spindle morphologic features and chromosome alignments.

Result(s): After culture for 22 to 24 hours, 77.1% of oocytes reached metaphase II stage, with 51.9% of oocytes showing birefringent spindles. Confocal microscopy revealed that 71% of oocytes with the birefringent spindles had normal chromosome alignment, and 29% of oocytes with birefringent spindles and all oocytes without birefringent spindles had abnormal microtubule organization and abnormal chromosome alignment.

Conclusion(s): The spindle images obtained with the PolScope in living human oocytes are coordinate with those in fixed oocytes as imaged by confocal microscopy. Spindle images with the PolScope can be applied to human in vitro fertilization to help predict chromosomally normal oocytes for insemination.

Keywords:  Human oocytes, in vitro maturation, PolScope, spindle