Fertility and Sterility
Volume 78, Issue 5 , Pages 1077-1081 , November 2002

Prediction of chromosome misalignment among in vitro matured human oocytes by spindle imaging with the PolScope

  • Wei-Hua Wang, Ph.D.

      Affiliations

    • Corresponding Author InformationReprint requests: Wei-Hua Wang, Ph.D., Houston Fertility Institute, 13414 Medical Complex Dr., Suite 7, Tomball, Texas, USA 77375 (FAX: 281-357-1865)
    • Houston Fertility Institute, Tomball Regional Hospital, Tomball, Texas, USA
    • ,
  • David L Keefe, M.D.

      Affiliations

    • Women and Infants Hospital of Rhode Island, USA

Received 2 January 2002 ,Revised 13 March 2002 ,Accepted 13 March 2002.

References 

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  10. Wang WH, Meng L, Hackett RJ, Keefe DL. Developmental ability of human eggs with or without birefringent spindles imaged by PolScope before insemination. Hum Reprod. 2001;16:1464–1468
  11. Wang WH, Meng L, Hackett RJ, Keefe DL. Rigorous thermal control during ICSI stabilizes the meiotic spindle and improves fertilization and pregnancy rates. Fertil Steril. 2002;77:1274–1277
  12. Cekleniak NA, Combelles AMH, Ganz DA, Fung J, Albertini DF, Rocowsky C. A novel system for in vitro maturation of human oocytes. Fertil Steril. 2001;75:1185–1193
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  14. de Matos DG, Furnus CC, Moses DF. Glutathione synthesis during in vitro maturation of bovine oocytes (role of cumulus cells). Biol Reprod. 1997;7:1420–1425
  15. Tatemoto H, Hakura N, Muto N. Protection of porcine oocytes against apoptotic cell death caused by oxidative stress during in vitro maturation (role of cumulus cells). Biol Reprod. 2000;63:805–810
  16. Sawai K, Funahashi H, Wang WH, Niwa K. Effect of cysteine in serum-free maturation medium on male pronuclear formation of maturing pig oocytes penetrated in vitro. J Reprod Dev. 1997;43:73–80
  17. Yamauchi N, Nagai T. Male pronuclear formation in denuded porcine oocytes after in vitro maturation in the presence of cysteamine. Biol Reprod. 1999;61:828–833
  18. Wang WH, Cao B, Meng L, Hackett RJ, Keefe DL. High proportions of meiotic spindles are abnormal in human oocytes as evidenced by birefringent spindle PolScope images in living eggs. 2001; 57th Annual Meeting of the American Society for Reproductive Medicine, October, abstract No. 5

PII: S0015-0282(02)04196-1

Fertility and Sterility
Volume 78, Issue 5 , Pages 1077-1081 , November 2002

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