Fertility and Sterility
Volume 79, Issue 3 , Pages 529-533 , March 2003

Intracytoplasmic sperm injection with testicular spermatozoa is less successful in men with nonobstructive azoospermia than in men with obstructive azoospermia

Presented at the 18th Annual Meeting of the European Society of Human Reproduction and Embryology; Vienna, Austria; June 30–July 3, 2002; nominated for the Promising Young Clinician Award by the ESHRE Scientific Committee.

  • Valérie Vernaeve, M.D.

      Affiliations

    • Center for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
    • Corresponding Author InformationReprint requests: Valérie Vernaeve, M.D., Center for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Laarbeeklaan 101, B-1090, Belgium (FAX: 32-2-477-65-49).
    • ,
  • Herman Tournaye, M.D., Ph.D.

      Affiliations

    • Center for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
    • ,
  • Kaan Osmanagaoglu, M.D.

      Affiliations

    • Center for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
    • ,
  • Greta Verheyen, Ph.D.

      Affiliations

    • Center for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
    • ,
  • André Van Steirteghem, M.D., Ph.D.

      Affiliations

    • Center for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
    • ,
  • Paul Devroey, M.D., Ph.D.

      Affiliations

    • Center for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium

Received 19 February 2002 ,Revised 24 July 2002 ,Accepted 24 July 2002.

References 

  1. Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic sperm injection of single spermatozoon into an oocyte. Lancet. 1992;340:17–18
  2. Tournaye H, Devroey P, Liu J, Nagy Z, Lissens W, Van Steirteghem A. Microsurgical epididymal sperm aspiration and intracytoplasmic sperm injection (a new effective approach to infertility as a result of congenital bilateral absence of the vas deferens). Fertil Steril. 1994;61:1445–1450
  3. Craft I, Bennett V, Nicholson N. Fertilising ability of testicular spermatozoa. Lancet 1993;342:864
  4. Silber SJ, Van Steirteghem AC, Liu J, Nagy Z, Tournaye H, Devroey P. High fertilization and pregnancy rate after intracytoplasmic sperm injection with spermatozoa obtained from testicle biopsy. Hum Reprod. 1995;10:148–152
  5. Nagy Z, Liu J, Janssenwillen C, Silber S, Devroey P, Van Steirteghem A. Using ejaculated, fresh, and frozen-thawed epididymal and testicular spermatozoa gives rise to comparable results after intracytoplasmic sperm injection. Fertil Steril. 1995;63:808–815
  6. Tournaye H, Camus M, Goossens A, Liu J, Nagy P, Silber S, et al.  Recent concepts in the management of infertility because of non-obstructive azoospermia. Hum Reprod. 1995;10(Suppl 1):115–119
  7. Devroey P, Liu J, Nagy Z, Goossens A, Tournaye H, Camus M, et al.  Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermia. Hum Reprod. 1995;10:1457–1460
  8. Schlegel PN, Palermo GD, Goldstein M, Menendez S, Zaninovic N, Veeck LL, et al.  Testicular sperm extraction with intracytoplasmic sperm injection for non-obstructive azoospermia. Urology. 1997;49:435–440
  9. Silber S, Van Steirteghem A, Nagy Z, Liu J, Tournaye H, Devroey P. Normal pregnancies resulting from testicular sperm extraction and intracytoplasmic sperm injection for azoospermia due to maturation arrest. Fertil Steril. 1996;55:110–117
  10. Palermo GD, Schlegel PN, Hariprashad JJ, Ergün B, Mielnik A, Zaninonvic N, et al.  Fertilization and pregnancy outcome with intracytoplasmic sperm injection for azoospermic men. Hum Reprod. 1999;14:741–748
  11. Mercan R, Urman B, Alatas C. Outcome of testicular sperm retrieval procedures in non-obstructive azoospermia (percutaneous aspiration versus open biopsy). Hum Reprod. 2000;15:1548–1551
  12. De Croo I, Van der Elst J, Everaert K, De Sutter P, Dhont M. Fertilization, pregnancy and embryo implantation rates after ICSI in cases of obstructive and non-obstructive azoospermia. Hum Reprod. 2000;15:1388–2000
  13. Balaban B, Urman B, Isiklar A, Atalas C, Meracan R, Aksoy S, et al.  Blastocyst transfer following intracytoplasmic injection of ejaculated, epididymal or testicular spermatozoa. Hum Reprod. 2001;16:125–129
  14. Bukulmez O, Yucel A, Yarali H, Bildirici I, Gurgan T. The origin of spermatozoa does not affect intracytoplasmic sperm injection outcome. Eur J Obstet Gynecol Reprod Biol. 2001;94:250–255
  15. Matsumiya K, Namiki M, Takahara S, Kondoh N, Takada S, Kiyohara H, et al.  Clinical study of azoospermia. Int J Androl. 1994;17:140–142
  16. Jow W, Steckel J, Schlegel P, Magid M, Goldstein M. Motile sperm in human testis biopsy specimens. J Androl. 1993;14:194–197
  17. Tournaye H, Camus M, Vandervorst M, Nagy Z, Joris H, Van Steirteghem A, et al.  Surgical sperm retrieval for intracytoplasmic sperm injection. Int J Androl. 1997;20(Suppl 3):69–73
  18. Verheyen G, De Croo I, Tournaye H, Pletincx I, Devroey P, Van Steirteghem AC. Comparison of four mechanical methods to retrieve spermatozoa from testicular tissue. Hum Reprod. 1995;10:2956–2959
  19. Crabbé E, Verheyen G, Silber S, Tournaye H, Van de Velde H, Goossens A, et al.  Enzymatic digestion of testicular tissue may rescue the intracytoplasmic sperm injection cycle in some patients with non-obstructive azoospermia. Hum Reprod. 1998;13:2791–2796
  20. Van Steirteghem AC, Nagy Z, Joris H, Liu J, Staessen C, Smitz J, et al.  High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod. 1993;8:1061–1066
  21. Joris H, Nagy Z, Van de Velde H, De Vos A, Van Steirteghem A. Intracytoplasmic sperm injection (laboratory set-up and injection procedure). Hum Reprod. 1998;13(Suppl 1):76–86
  22. Salzbrunn A, Benson DM, Hostein AF, Schulze W. A new concept for the extraction of testicular spermatozoa as a tool for assisted fertilization (ICSI). Hum Reprod. 1996;11:752–755
  23. Westlander G, Hamberger L, Hanson CH, Lundi K, Nilsson L, Söderlund B, et al.  Diagnostic epididymal and testicular sperm recovery and genetic aspects in azoospermic men. Hum Reprod. 1999;14:118–122
  24. Turek P, Givens C, Schriock E, Meng M, Pedersen R, Conaghan J. Testis sperm extraction and intracytoplasmic sperm injection guided by prior fine-needle aspiration mapping in patients with non obstructive azoospermia. Fertil Steril. 1999;71:552–557
  25. Martin RH, Greene C, Rademaker A, Barclay L, Ko E, Chernos J. Chromosome analysis of spermatozoa extracted from testes of men with non-obstructive azoospermia. Hum Reprod. 2000;15:1121–1124
  26. Bernardini L, Gianaroli L, Fortini D, Conte N, Magli C, Cavani S, et al.  Frequency of hyper-, hypohaploidy and diploidy in ejaculated, epididymal and testicular germ cells of infertile patients. Hum Reprod. 2000;15:2165–2172
  27. Levron J, Aviram-Goldring A, Madgar I, Raviv G, Barkai G, Dor J. Sperm chromosome abnormalities in men with severe male factor infertility who are undergoing in-vitro fertilization with intracytoplasmic sperm injection. Fertil Steril. 2001;76:479–484

☆ Supported by grants from the Belgian Fund for Medical Research, Flanders, Belgium.

PII: S0015-0282(02)04809-4

doi: 10.1016/S0015-0282(02)04809-4

Fertility and Sterility
Volume 79, Issue 3 , Pages 529-533 , March 2003

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