Fertility and Sterility
Volume 81, Issue 2 , Pages 408-415 , February 2004

Fast multicolor primed in situ protocol for chromosome identification in isolated cells may be used for human oocytes and polar bodies

  • Franck Pellestor, Ph.D.

      Affiliations

    • Institut of Human Genetics, CNRS-UPR 1142, Montpellier, France
    • Corresponding Author InformationReprint requests: Franck Pellestor, Ph.D., CNRS-UPR 1142, IGH, 141, rue de la Cardonille, F-34396 Montpellier cedex 5, France (FAX: 33-0-4-99-61-99-01).
    • ,
  • Tal Anahory, M.D.

      Affiliations

    • Cytogenetic Laboratory, C.H.U. Arnaud de Villeneuve, Montpellier, France
    • ,
  • Brigitte Andréo, M.Sc.

      Affiliations

    • Institut of Human Genetics, CNRS-UPR 1142, Montpellier, France
    • ,
  • Gilles Régnier-Vigouroux, M.D.

      Affiliations

    • IVF Laboratory, Clinique St. Roch, Montpellier, France
    • ,
  • Jean Pierre Soulié, M.D.

      Affiliations

    • IVF Laboratory, Clinique St. Roch, Montpellier, France
    • ,
  • Magalie Baudouin, M.Sc.

      Affiliations

    • IVF Laboratory, Clinique St. Roch, Montpellier, France
    • ,
  • Jacques Demaille, M.D.

      Affiliations

    • Institut of Human Genetics, CNRS-UPR 1142, Montpellier, France

Received 30 December 2002 ,Revised 15 August 2003 ,Accepted 15 August 2003.

References 

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  2. Egozcue J, Blanco J, Vidal F. Chromosome studies in human sperm nuclei using fluorescence in-situ hybridization (FISH). Hum Reprod Update. 1997;3:441–452
  3. Koch JE, Kolvraa S, Petersen KB, Gregersen N, Bolund L. Oligonucleotide-priming methods for the chromosome-specific labelling of alpha satellite DNA in situ. Chromosoma. 1989;98:259–265
  4. Orsetti B, Lefort G, Boulot P, Andreo B, Pellestor F. Fetal cells in maternal blood (the use of primed in situ (PRINS) labelling technique for fetal cell detection and sex assessment). Prenat Diagn. 1998;18:1014–1022
  5. Velagelati GV, Shulman LP, Phillips OP, Tharapel SA, Tharapel AT. Primed in situ labeling for rapid prenatal diagnosis. Am J Obstet Gynecol. 1998;178:1313–1320
  6. Pellestor F, Girardet A, Coignet L, Andreo B, Charlieu JP. Assessment of aneuploidy for chromosomes 8, 9, 13, 16, and 21 in human sperm by using primed in situ labeling technique. Am J Hum Genet. 1996;58:797–802
  7. Petit C, Martel-Petit V, Fleurentin A, Monnier-Barbarino P, Jonveaux P, Gerard H. Use of PRINS for preconception screening of polar bodies for common aneuploidies. Prenat Diagn. 2000;20:1067–1071
  8. Krabchi K, Gros-Louis F, Yan J, Bronsard M, Masse J, Forest JC, et al.  Quantification of all fetal nucleated cells in maternal blood between the 18th and 22nd weeks of pregnancy using molecular cytogenetic techniques. Clin Genet. 2001;60:145–150
  9. Yan J, Bronsard M, Drouin R. Creating a new color by omission of 3 end blocking step for simultaneous detection of different chromosomes in multi-PRINS technique. Chromosoma. 2001;109:565–570
  10. Tarkowski A. An air-drying method for chromosome preparation from mouse eggs. Cytogenetics. 1966;5:394–400
  11. Coullin P, Andreo B, Charlieu JP, Candelier JJ, Pellestor F. Primed in situ (PRINS) labelling with Alu and satellite primers for rapid characterization of human chromosomes in hybrid cell lines. Chromosome Res. 1997;5:307–312
  12. Kasten FH. Introduction to fluorescent probes: properties, history and applications. In:  Mason WT editors. Fluorescent and luminescent probes for biological activity. New York: Academic Press; 1993;p. 12–33
  13. Haugland RP. Handbook of fluorescent probes and research chemicals. Leiden, The Netherlands: Molecular Probes; 1996;
  14. Pellestor F, Girardet A, Andreo B, Charlieu JP. A polymorphic alpha satellite sequence specific for human chromosome 13 detected by oligonucleotide primed in situ labelling (PRINS). Hum Genet. 1994;94:346–348
  15. Krejci K, Koch J. An in situ study of variant telomeric repeats in human chromosomes. Genomics. 1999;58:202–206
  16. Wachtel SS, Tharapel AT. FISH and PRINS (competing or complementary technologies?). Am J Med Genet. 2002;107:97–98
  17. Mahmood R, Brierley CH, Faed MJ, Mills JA, Delhanty JD. Mechanisms of maternal aneuploidy (FISH analysis of oocytes and polar bodies in patients undergoing assisted conception). Hum Genet. 2000;106:620–626
  18. Martini E, Flaherty SP, Swann NJ, Matthews CD, Ramaekers FC, Geraedts JP. FISH analysis of six chromosomes in unfertilized human oocytes after polar body removal. J Assist Reprod Genet 2000;17:276–93
  19. Marquez C, Cohen J, Munne S. Chromosome identification in human oocytes and polar bodies by spectral karyotyping. Cytogenet Cell Genet. 1998;81:254–258
  20. Fung J, Weier HU, Goldberg JD, Pedersen RA. Multilocus genetic analysis of single interphase cells by spectral imaging. Hum Genet. 2000;107:615–622
  21. Clyde JM, Gosden RG, Rutherford AJ, Picton HM. Demonstration of a mechanism of aneuploidy in human oocytes using Multifluor fluorescence in situ hybridization. Fertil Steril. 2001;76:837–840
  22. Sandalinas M, Marquez C, Munne S. Spectral karyotyping of fresh, non-inseminated oocytes. Mol Hum Reprod. 2002;8:580–585
  23. He ZY, Liu HC, Mele CA, Veeck LL, Davis O, Rosenwaks Z. Recycling of a single human blastomere fixed on a microscopic slide for sexing and diagnosis of specific mutations by various types of polymerase chain reaction. Fertil Steril. 1999;72:341–348

☆ Supported by a European grant, COPERNICUS 2 (contract ICA2-CT-2000-10012, proposal ICA2-1999-20007).

PII: S0015-0282(03)02880-2

doi: 10.1016/j.fertnstert.2003.08.014

Fertility and Sterility
Volume 81, Issue 2 , Pages 408-415 , February 2004

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