Pregnancy after cryopreservation of donor oocytes and preimplantation genetic diagnosis of embryos in a patient with ovarian failure

Miller, Kathleen A; Elkind-Hirsch, Karen; Levy, Brynn; Graubert, Michael D; Ross, Sudeep J; Scott, Richard T

doi:10.1016/j.fertnstert.2003.12.031

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Volume 82, Issue 1 , Pages 211-214, July 2004

Pregnancy after cryopreservation of donor oocytes and preimplantation genetic diagnosis of embryos in a patient with ovarian failure

Presented in part at the 59th Annual Meeting of the American Society for Reproductive Medicine, San Antonio, Texas, October 11–15, 2003.

Kathleen A Miller, B.S.
- Reprint requests: Kathleen A. Miller, Reproductive Medicine Associates of New Jersey, 111 Madison Avenue, Suite 100, Morristown, New Jersey 07962, USA (FAX: 973-290-8370).
- Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
Karen Elkind-Hirsch, M.Sc., Ph.D.
- Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
Brynn Levy, M.Sc. (Med)., Ph.D.
- Mount Sinai School of Medicine, New York, New York, USA
Michael D Graubert, M.D.
- Palmetto Fertility Center of South Florida, Miami, Florida, USA
Sudeep J Ross, M.D.
- Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
Richard T Scott Jr., M.D.
- Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA

Received 10 September 2003; received in revised form 8 December 2003; accepted 8 December 2003.

Abstract

Objective

To describe the successful use of preimplantation genetic diagnosis to assess the prevalence of meiotic errors after oocyte cryopreservation in an oocyte donation cycle.

Design

Case report.

Setting

Private IVF center.

Patient(s)

A 42.6-year-old patient with ovarian failure.

Intervention(s)

A donor oocyte IVF cycle with cryopreservation of oocytes followed by thaw, fertilization of oocytes, preimplantation genetic diagnosis for selective aneuploidy, and ET.

Main outcome measure(s)

Preimplantation genetic analysis of chromosomes 13,16,18, 21,22, X, and Y with fluorescence in-situ hybridization.

Result(s)

The recipient's initial serum β-hCG level was 196 mIU/mL 15 days after oocyte retrieval. An initial ultrasound at the sixth week of gestation revealed two gestational sacs. A second ultrasound 1 week later showed a monochorionic twin in sac A and a singleton pregnancy in sac B. Fetal cardiac activity was visualized for all gestations.

Conclusion(s)

This case illustrates the feasibility of cryopreservation of donor oocytes combined with preimplantation genetic diagnosis for clinical use in those settings where there may be an increased risk of spindle-related abnormalities.

Keywords: Cryopreserved oocytes, preimplantation genetic diagnosis, aneuploidy, pregnancy, FISH