Fertility and Sterility
Volume 82, Issue 4 , Pages 919-922 , October 2004

Fluorescence in situ hybridization analysis of human oocytes: Advantages of a double-labeling procedure

  • Franck Pellestor, Ph.D.

      Affiliations

    • CNRS-UPR 1142, Montpellier, France
    • Corresponding Author InformationReprint requests: Franck Pellestor, Ph.D., CNRS-UPR 1142, IGH, 141, rue de la Cardonille, F-34396 Montpellier Cedex 5, France (FAX: 33-499-61-99-01
    • ,
  • Tal Anahory, M.D.

      Affiliations

    • Cytogenetic Laboratory, C.H.U. Arnaud de Villeneuve, Montpellier, France
    • ,
  • Brigitte Andréo, M.Sc.

      Affiliations

    • CNRS-UPR 1142, Montpellier, France
    • ,
  • Gilles Régnier-Vigouroux, M.D.

      Affiliations

    • IVF Laboratory, Clinique St. Roch, Montpellier, France
    • ,
  • Jean Pierre Soulié, M.D.

      Affiliations

    • IVF Laboratory, Clinique St. Roch, Montpellier, France
    • ,
  • Magalie Baudouin, M.Sc.

      Affiliations

    • IVF Laboratory, Clinique St. Roch, Montpellier, France
    • ,
  • Jacques Demaille, M.D.

      Affiliations

    • CNRS-UPR 1142, Montpellier, France

Received 12 June 2003 ,Revised 3 March 2004 ,Accepted 3 March 2004.

References 

  1. Dailey T , Dale B , Cohen J , Munné S . Association between nondisjunction and maternal age in meiosis-II human oocytes . Am J Hum Genet . 1996;59:176–184
  2. Martini E , Flaherty SP , Swann JN , Matthews CD , Ramaekers FC , Geraedts JP . FISH analysis of six chromosomes in unfertilized human oocytes after polar body removal . J Assist Reprod Genet . 2000;17:276–283
  3. Mahmood R , Brierley CH , Faed MJ , Mills JA , Delhanty JD . Mechanisms of maternal aneuploidy: FISH analysis of oocytes and polar bodies in patients undergoing assisted conception . Hum Genet . 2000;106:620–626
  4. Pujol A , Boiso I , Benet J , Veiga A , Durban M , Campillo M , et al.  Analysis of nine chromosome probes in first polar bodies and metaphase II oocytes for the detection of aneuploidies . Eur J Hum Genet . 2003;11:325–336
  5. Tarkowski A . An air-drying method for chromosome preparation from mouse eggs . Cytogenetics . 1966;5:394–400
  6. Munné S , Weier HUG . Simultaneous enumeration of chromosomes 13, 18, 21, X and Y in interphase cells for preimplantation genetic diagnosis of aneuploidy . Cytogenet Cell Genet . 1996;75:263–270
  7. Marquez C , Cohen J , Munné S . Chromosome identification in human oocytes and polar bodies by spectral karyotyping . Cytogenet Cell Genet . 1998;81:254–258
  8. Sandalinas M , Marquez C , Munné S . Spectral karyotyping of fresh, non-inseminated oocytes . Mol Hum Reprod . 2002;8:580–585
  9. Clyde JM , Hogg JE , Rutherford AJ , Picton HM . Karyotyping of human metaphase II oocytes by Multifluor fluorescence in situ hybridization . Fertil Steril . 2003;80:1003–1011

 Supported by a European grant COPERNICUS 2 (Contract ICA2-CT-2000-10012, proposal ICA2-1999-20007).

PII: S0015-0282(04)01279-8

doi: 10.1016/j.fertnstert.2004.03.050

Fertility and Sterility
Volume 82, Issue 4 , Pages 919-922 , October 2004

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