Fertility and Sterility
Volume 82, Issue 4 , Pages 960-962 , October 2004

An optimized protocol of a human-to-cattle interspecies somatic cell nuclear transfer

  • Kyung H. Chang, M.S.

      Affiliations

    • School of Agricultural Biotechnology, Seoul National University, Seoul, Korea
    • ,
  • Jeong M. Lim, D.V.M., Ph.D.

      Affiliations

    • College of Veterinary Medicine, Seoul National University, Seoul, Korea
    • Corresponding Author InformationReprint requests: Jeong M. Lim, D.V.M., Ph.D., School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea (FAX: 822-874-2555
    • ,
  • Sung K. Kang, D.V.M., Ph.D.

      Affiliations

    • Medical Research Center, College of Medicine,d Seoul National University, Seoul, Korea
    • ,
  • Byeong C. Lee, D.V.M., Ph.D.

      Affiliations

    • Medical Research Center, College of Medicine,d Seoul National University, Seoul, Korea
    • ,
  • Shin Y. Moon, M.D., Ph.D.

      Affiliations

    • College of Veterinary Medicine, Seoul National University, Seoul, Korea
    • Stem Cell Research Center, Seoul, Korea
    • ,
  • Woo S. Hwang, D.V.M., Ph.D.

      Affiliations

    • Medical Research Center, College of Medicine,d Seoul National University, Seoul, Korea

Received 17 October 2003 ,Revised 9 March 2004 ,Accepted 9 March 2004.

References 

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  2. Corley Smith GE , Brandhorst BP . Preservation of endangered species and populations: a role for genome banking, somatic cell cloning, and androgenesis? . Mol Reprod Dev . 1999;53:363–367
  3. Dominko T , Mitalipova M , Haley B . Bovine oocyte cytoplasm supports development of embryos produced by nuclear transfer of somatic cell nuclei from various mammalian species . Biol Reprod . 1999;60:1496–1502
  4. Lanza RP , Civelli JB , Diaz F . Cloning of an endangered species (Bos gaurus) using interspecies nuclear transfer . Cloning . 2000;2:79–90
  5. Kitiyanant Y , Saiklun J , Chaisalee B , White KL , Pavasuthipaisit K . Somatic cell cloning in buffalo (Bubalus bubalis): eggets of interspecies cytoplasmic recipient and activation procedures . Cloning Stem Cells . 2001;3:97–9104
  6. Chen D , Wen D , Zhang Y , Sun QY , Han ZM , Liu ZH , et al.  Interspecies implantation and mitochondria fate of Panda-Rabbit cloned embryos . Biol Reprod . 2002;67:637–642
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  8. Chang KH , Lim JM , Kang SK , Kim DY , Lee BC , Cha SH , et al.  Preimplantation development and cytological characterization of interspecies embryos generated by human cord fibroblast nuclear transfer into bovine enucleated oocytes . Fertil Steril . 2003;80:1380–1387
  9. Betteridge KJ . Phylogeny, ontogeny and embryo transfer . Theriogenology . 1995;44:1061–1098
  10. Statistical Analysis System Institute . SAS user's guide. Statistics . Cary, NC: Statistical Analysis System Institute; 1990;
  11. Choi YH , Lee BC , Lim JM , Kang SK , Hwang WS . Optimization of culture medium for cloned bovine embryos and its influence on pregnancy and delivery outcome . Theriogenology . 2002;58:1187–1197
  12. Kopecny V , Flechon JE , Camous S , Fulka JJr. Nucleogenesis and the onset of transcription in the eight-cell bovine embryo: fine-structural autoradiographic study . Mol Reprod Dev . 1989;1:79–90
  13. Tesarik J , Kopecny V , Plachot M , Mandelbaum J. Activation of nucleolar and extranucleolar RNA synthesis and changes in the ribosomal content of human embryos developing in vitro . J Reprod Fertil . 1986;78:463–470

 Supported by a graduate fellowship provided by the Korean Ministry of Education through the BK 21 project, Seoul, and by a grant from Stem Cell Research Center (SC11011).

PII: S0015-0282(04)01291-9

doi: 10.1016/j.fertnstert.2004.03.052

Fertility and Sterility
Volume 82, Issue 4 , Pages 960-962 , October 2004

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