Fertility and Sterility
Volume 82, Issue 6 , Pages 1654-1659 , December 2004

Simultaneous analysis of cytoskeletal patterns and chromosome positioning in human fertilization failures

  • João Ramalho-Santos, Ph.D.

      Affiliations

    • Center for Neuroscience and Cell Biology, Department of Zoology, University of Coimbra, Coimbra, Portugal
    • Corresponding Author InformationReprint requests: João Ramalho-Santos, Ph.D., Center for Neuroscience and Cell Biology, Department of Zoology, University of Coimbra, 3004-517 Coimbra, Portugal (FAX: + 351-239-826798
    • ,
  • Alexandra Amaral

      Affiliations

    • Center for Neuroscience and Cell Biology, Department of Zoology, University of Coimbra, Coimbra, Portugal
    • ,
  • Raquel Brito

      Affiliations

    • Department of Maternal-Fetal Medicine, Genetics and Human Reproduction, University Hospitals of Coimbra, Coimbra, Portugal
    • ,
  • Mariana Freitas, M.Sc.

      Affiliations

    • Center for Neuroscience and Cell Biology, Department of Zoology, University of Coimbra, Coimbra, Portugal
    • ,
  • Teresa Almeida Santos, M.D., Ph.D.

      Affiliations

    • Department of Maternal-Fetal Medicine, Genetics and Human Reproduction, University Hospitals of Coimbra, Coimbra, Portugal

Received 7 January 2004 ,Revised 4 May 2004 ,Accepted 4 May 2004.

References 

  1. Trounson AO , Gosden RG . Biology and pathology of the oocyte: its role in fertility and reproductive medicine . , eds. Cambridge, UK: Cambridge University Press; 2003;
  2. Asch R , Simerly C , Ord T , Ord VA , Schatten G . The stages at which human fertilization arrests: microtubule and chromosome configurations in inseminated oocytes which failed to complete fertilization and development in humans . Hum Reprod . 1995;10:1897–1906
  3. Coonen E , Hopman AHN , Geraedts JPM , Remaekers CS . Application of in-situ hybridization techniques to study human preimplantation embryos: a review . Hum Reprod Update . 1998;4:135–152
  4. Schatten G . The centrosome and its mode of inheritance: the reduction of the centrosome during gametogenesis and its restoration during fertilization . Dev Biol . 1994;165:299–335
  5. Hassold T , Hunt P . To err (meiotically) is human: the genesis of human aneuploidy . Nat Rev Genet . 2001;2:280–291
  6. Barlow AL , Tease C , Hulten MA . Meiotic chromosome pairing in fetal oocytes of trisomy 21 human females . Cytogenet Genome Res . 2002;96:45–51
  7. Hodges CA , LeMaire-Adkins R , Hunt PA . Coordinating the segregation of sister chromatids during the first meiotic division: evidence for sexual dimorphism . J Cell Sci . 2001;114:2417–2426
  8. Hodges CA , Hunt PA . Simultaneous analysis of chromosomes and chromosome-associated proteins in mammalian oocytes and embryos . Chromosoma . 2002;111:165–169
  9. Tease C , Hartshorne GM , Hulten MA . Patterns of meiotic recombination in human fetal oocytes . Am J Hum Genet . 2002;70:1469–1479
  10. Hunt P , LeMaire R , Embury P , Sheean L , Mroz K . Analysis of chromosome behavior in intact mammalian oocytes: monitoring the segregation of a univalent chromosome during female meiosis . Hum Mol Genet . 1995;4:2007–2012
  11. LeMaire-Adkins R , Radke K , Hunt PA . Lack of checkpoint control at the metaphase/anaphase transition: a mechanism of meiotic nondisjunction in mammalian females . J Cell Biol . 1997;139:1611–1619
  12. LeMaire-Adkins R , Hunt PA . Nonrandom segregation of the mouse univalent X chromosome: evidence of spindle-mediated meiotic drive . Genetics . 2000;156:775–783
  13. Simerly C , Moreno RD , Ramalho-Santos J , Hewitson L , Schatten G . Confocal imaging of structural molecules in mammalian gametes . In:  Gardner DK ,  Lane M editor. A laboratory guide of the mammalian embryo . Oxford, UK: Oxford University Press; 2003;
  14. Ramalho-Santos J , Sutovsky P , Simerly C , Oko R , Wessel G , Hewitson L , et al.   ICSI choreography: fate of sperm structures after monospermic rhesus ICSI and first cell cycle implications . Hum Reprod . 2000;15:2610–2620

 Supported by a grant from the Portuguese National Science Foundation (Fundação para a Ciência e Tecnologia, FCT) to J. R.-S. (POCTI/ESP/38049/2001). A.A. is supported by a Ph.D. grant from FCT, Portugal (SFRH/BD/12665/2003).

PII: S0015-0282(04)02411-2

doi: 10.1016/j.fertnstert.2004.05.086

Fertility and Sterility
Volume 82, Issue 6 , Pages 1654-1659 , December 2004

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