Fertility and Sterility
Volume 83, Issue 6 , Pages 1687-1694 , June 2005

Toward using stable spermatozoal RNAs for prognostic assessment of male factor fertility

  • G. Charles Ostermeier, Ph.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan
    • Center for Molecular Medicine and Genetics, Wayne State University, Detroit, Michigan
    • ,
  • Robert J. Goodrich, B.S.

      Affiliations

    • Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan
    • Center for Molecular Medicine and Genetics, Wayne State University, Detroit, Michigan
    • ,
  • Michael P. Diamond, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan
    • ,
  • David J. Dix, Ph.D.

      Affiliations

    • Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina
    • ,
  • Stephen A. Krawetz, Ph.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan
    • Center for Molecular Medicine and Genetics, Wayne State University, Detroit, Michigan
    • Center for Scientific Computing, Wayne State University, Detroit, Michigan
    • Corresponding Author InformationReprint requests: Stephen A. Krawetz, Ph.D., 253 C.S. Mott Center, 275 East Hancock, Detroit, MI 48201 (FAX: 313-577-8554)

Received 15 September 2004 ,Revised 15 December 2004 ,Accepted 15 December 2004.

References 

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  9. Saacke RG , Dalton JC , Nadir S , Nebel RL , Bame JH . Relationship of seminal traits and insemination time to fertilization rate and embryo quality . Anim Reprod Sci . 2000;663–677 60–61
  10. Evenson DP . Loss of livestock breeding efficiency due to uncompensable sperm nuclear defects . Reprod Fertil Dev . 1999;11:1–15
  11. Kierszenbaum AL , Tres LL . Structural and transcriptional features of the mouse spermatid genome . J Cell Biol . 1975;65:258–270
  12. Miller D , Briggs D , Snowden H , Hamlington J , Rollinson S , Lilford R , et al.   A complex population of RNAs exists in human ejaculate spermatozoa (implications for understanding molecular aspects of spermiogenesis) . Gene . 1999;237:385–392
  13. Ostermeier GC , Dix DJ , Miller D , Khatri P , Krawetz SA . Spermatozoal RNA profiles of normal fertile men . Lancet . 2002;360:772–777
  14. Wang H, Zhou Z, Xu M, Li J, Xiao J, Xu ZY, et al. A spermatogenesis-related gene expression profile in human spermatozoa and its potential clinical applications . J Mol Med . 2004;82:317–324
  15. Lambard S , Galeraud-Denis I , Martin G , Levy R , Chocat A , Carreau S . Analysis and significance of mRNA in human ejaculated sperm from normozoospermic donors (relationship to sperm motility and capacitation) . Mol Hum Reprod . 2004;10:535–541
  16. Moldenhauer JS , Ostermeier GC , Johnson A , Diamond MP , Krawetz SA . Diagnosing male factor infertility using microarrays . J Androl . 2003;24:783–789
  17. Ostermeier GC , Goodrich RJ , Moldenhauer JS , Diamond MP , Krawetz SA . A suite of novel human spermatozoal RNAs . J Androl . 2005;26:70–74
  18. Ostermeier GC , Miller D , Huntriss JD , Diamond MP , Krawetz SA . Reproductive biology (delivering spermatozoan RNA to the oocyte) . Nature . 2004;429:154
  19. Goodrich RJ , Ostermeier GC , Krawetz SA . Multitasking with molecular dynamics Typhoon (quantifying nucleic acids and autoradiographs) . Biotechnol Lett . 2003;25:1061–1065
  20. Ostermeier GC , Dix DJ , Krawetz SA . A bioinformatic strategy to rapidly characterize cDNA libraries . Bioinformatics . 2002;18:949–952
  21. Conover WJ , et al.   Practical nonparametric statistics. Some tests based on the binomial distribution . New York, Chichester, Brisbane, Toronto, Singapore: John Wiley & Sons; 1980; 95–142.
  22. Medeiros CM , Forell F , Oliveira AT , Rodrigues JL . Current status of sperm cryopreservation (why isn’t it better?) . Theriogenology . 2002;57:327–344
  23. Hammadeh ME , Georg T , Rosenbaum P , Schmidt W . Association between freezing agent and acrosome damage of human spermatozoa from subnormal and normal semen . Andrologia . 2001;33:331–336
  24. Watson PF . The causes of reduced fertility with cryopreserved semen . Anim Reprod Sci . 2000;481–492 60–61
  25. Rockett JC , Patrizio P , Schmid JE , Hecht NB , Dix DJ . Gene expression patterns associated with infertility in humans and rodent models . Mutat Res . 2004;549:225–240
  26. Wykes SM , Visscher DW , Krawetz SA . Haploid transcripts persist in mature human spermatozoa . Mol Hum Reprod . 1997;3:159

 Supported by WSU Ob/Gyn grant 95200 to S.A.K. The information presented in this document has been funded in part by the U.S. Environmental Protection Agency. It has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. Normal fertile human semen samples were a generous gift from the NICHD Reproductive Medicine Network (U10 HD-39005).

PII: S0015-0282(05)00541-8

doi: 10.1016/j.fertnstert.2004.12.046

Fertility and Sterility
Volume 83, Issue 6 , Pages 1687-1694 , June 2005

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