Fertility and Sterility
Volume 84, Issue 3 , Pages 584-589 , September 2005

Nuclear status of four-cell preembryos predicts implantation potential in in vitro fertilization treatment cycles

  • Pia Saldeen, M.D., Ph.D.

      Affiliations

    • Corresponding Author InformationReprint requests: Pia Saldeen, M.D., Ph.D., IVF Clinic CURA, Box 200 37, S-200 74 Malmö, Sweden (FAX: 4640987055).
  • ,
  • Per Sundström, M.D., Ph.D.

Received 22 December 2004 ,Revised 29 March 2005 ,Accepted 29 March 2005.

References 

  1. Scott LA , Smith S . The successful use of pronuclear embryo transfers the day following oocyte retrieval . Hum Reprod . 1998;13:1003–1013
  2. Tesarik J , Greco E . The probability of abnormal preimplantation development can be predicted by a single static observation on pronuclear stage morphology . Hum Reprod . 1999;14:1318–1323
  3. Cummins JM , Breen TM , Harrison KL , Shah JM , Wilson LM , Hennessey JF . A formula for scoring human embryo growth rates in in vitro fertilization (its value in predicting pregnancy and in comparison with visual estimates of embryo quality) . J In Vitro Fert Embryo Transf . 1986;3:284–295
  4. Ziebe S , Petersen K , Lindenberg S , Andersen AG , Gabrielsen A , Nyboe Andersen A . Embryo morphology or cleavage stage (how to select the best embryos for transfer after in vitro-fertilization) . Hum Reprod . 1997;12:1545–1549
  5. Hardarson T , Hanson C , Sjögren A , Lundin K . Human embryos with unevenly sized blastomeres have lower pregnancy and implantation rates (indications for aneuploidy and multinucleation) . Hum Reprod . 2001;16:313–318
  6. Moriwaki T , Suganuma N , Hayakawa M , Hibi H , Katsumata Y , Oguchi H , et al.   Embryo evaluation by analysing blastomere nuclei . Hum Reprod . 2004;19:152–156
  7. Sakkas D , Shoukir Y , Chardonnens D , Bianchi PG , Campana A . Early cleavage of human embryos to the two cell-stage after intracytoplasmic sperm injection as an indicator of embryo viability . Hum Reprod . 1998;13:182–187
  8. Lundin K , Bergh C , Hardarson T . Early embryo cleavage is a strong indicator of embryo quality in human IVF . Hum Reprod . 2001;16:2652–2657
  9. Palmstierna M , Murkes D , Csemiczky G , Andersson O , Wramsby H . Zona pellucida thickness variation and occurrence of visible mononucleated blastomeres in preembryos are associated with a high pregnancy rate in IVF treatment . Assist Reprod Genet . 1998;15:70–75
  10. Pickering SJ , Taylor A , Johnson MH , Braude PR . An analysis of multinucleated blastomere formation in human embryos . Hum Reprod . 1995;10:1912–1922
  11. Kligman I , Benadiva C , Alikani M , Munne S . The presence of multinucleated blastomeres in human embryos is correlated with chromosomal abnormalities . Hum Reprod . 1996;11:1492–1498
  12. Balakier H , Cadesky K . The frequency and developmental capability of human embryos containing multinucleated blastomeres . Hum Reprod . 1997;12:800–804
  13. Jackson KV , Ginsbury ES , Hornstein MD , Rein MS , Clarke RN . Multinucleation in normally fertilized embryos is associated with an accelerated ovulation induction response and lower implantation and pregnancy rates in in vitro fertilization-embryo transfer cycles . Fertil Steril . 1998;70:60–66
  14. Pelinck MJ , De Vos M , Dekens M , Van der Elst J , De Sutter P , Dhont M . Embryos cultured in vitro with multinucleated blastomeres have poor implantation potential in human in-vitro fertilization and intracytoplasmatic sperm injection . Hum Reprod . 1998;13:960–963
  15. Van Royen E , Mangelshots K , Vercruyssen M , De Neubourg D , Valkenburg M , Ryckaert G , et al.   Multinucleation in cleavage stage embryos . Hum Reprod . 2003;18:1062–1069
  16. Magli MC , Gianaroli L , Ferraretti AP . Chromosomal abnormalities in embryos . Mol Cell Endocrinol . 2001;183(Suppl 1):29–34
  17. Van Royen E , Mangelshots K , De Neubourg D , Valkenburg M , Van de Meerssche M , Ryckaert G , et al.   Characterization of a top quality embryo, a step towards single embryo transfer . Hum Reprod . 1999;14:2345–2349
  18. Saldeen P , Sundström P . Would legislation imposing single embryo transfer be a feasible way to reduce the rate of multiple pregnancies after IVF treatment? . Hum Reprod . 2005;20:4–8
  19. Gardner DK , Lane M . Towards a single embryo transfer . Reprod Biomed Online . 2003;6:470–481
  20. Gardner DK , Surrey E , Minjarez D , Leitz A , Stevens J , Schoolcraft WB . Single blastocyst transfer (a prospective randomized trial) . Fertil Steril . 2004;81:551–555
  21. Milki AA , Hinckley MD , Westphal LM , Behr B . Elective single blastocyst transfer . Fertil Steril . 2004;81:1697–1698
  22. Milki AA , Jun SH , Hinckley MD , Behr B , Giudice LC , Westphal LM . Incidence of monozygotic twinning with blastocyst transfer compared to cleavage-stage transfer . Fertil Steril . 2003;79:503–506
  23. Gardner DK . Report of the meeting. Infertility therapy-associated multiple pregnancies (births): an ongoing epidemic. Role of blastocyst transfer . Reprod Biomed Online . 2003;7(Supp 2):5–32
  24. Blake D , Proctor M , Johnson N , Olive D . Cleavage stage versus blastocyst stage embryo transfer in assisted conception . Cochrane Database Syst Rev . 2002;2: CD002118
  25. Gerris J . Reducing the number of embryos to transfer after IVF/ICSI . In:  Van Blerkom J ,  Gregory L editor. Essential IVF (basic research and clinical applications) . Boston: Kluwer Academic; 2004;p. 505–554

PII: S0015-0282(05)01095-2

doi: 10.1016/j.fertnstert.2005.03.059

Fertility and Sterility
Volume 84, Issue 3 , Pages 584-589 , September 2005