Fertility and Sterility
Volume 84, Issue 6 , Pages 1665-1673, December 2005

Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidies

Presented in part at the 2002 Meeting of the European Society for Human Reproduction and Embryology, Vienna, Austria, July 1–3, 2002.

  • Attila Jakab, M.D.

      Affiliations

    • Sperm Physiology Laboratory, Yale University School of Medicine, New Haven, Connecticut, USA
    • ,
  • Denny Sakkas, Ph.D.

      Affiliations

    • In Vitro Fertilization Laboratory, Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut, USA
    • ,
  • Elena Delpiano, M.D.

      Affiliations

    • Sperm Physiology Laboratory, Yale University School of Medicine, New Haven, Connecticut, USA
    • ,
  • Sevil Cayli, M.S.

      Affiliations

    • Sperm Physiology Laboratory, Yale University School of Medicine, New Haven, Connecticut, USA
    • ,
  • Ertug Kovanci, M.D.

      Affiliations

    • Sperm Physiology Laboratory, Yale University School of Medicine, New Haven, Connecticut, USA
    • ,
  • David Ward, Ph.D.

      Affiliations

    • Department of Genetics, Yale University School of Medicine, New Haven, Connecticut
    • ,
  • Alberto Ravelli, M.D.

      Affiliations

    • Sperm Physiology Laboratory, Yale University School of Medicine, New Haven, Connecticut, USA
    • ,
  • Gabor Huszar, M.D.

      Affiliations

    • Sperm Physiology Laboratory, Yale University School of Medicine, New Haven, Connecticut, USA
    • Corresponding Author InformationReprint requests: Gabor Huszar, M.D., Yale School of Medicine, Department of Obstetrics and Gynecology, 333 Cedar Street, New Haven, Connecticut 06520 (FAX: 203-737-1200).

Received 25 January 2005; received in revised form 24 May 2005; accepted 24 May 2005.

Objective

To test a newly invented intracytoplasmic sperm injection (ICSI) sperm selection method based on sperm hyaluronic acid (HA) binding.

Design

Comparison of chromosomal disomy and diploidy frequencies in sperm arising from semen and in HA-bound sperm.

Setting

Academic andrology laboratory.

Patient(s)

Men presenting for semen analysis.

Intervention(s)

Washed sperm fractions of 32 semen samples were applied to Petri dishes or glass slides coated with immobilized HA. The unbound sperm were rinsed gently, and the HA-bound sperm were removed with an ICSI pipette. The control sperm population was the unselected sperm. Both HA-selected and unselected sperm were treated with fluorescence in situ hybridization with centromeric probes for the X, Y, and 17 chromosomes.

Main Outcome Measure(s)

Chromosomal disomy and diploidy frequencies.

Result(s)

In the HA-bound sperm (495–2,079 per man, 41,670 in all) compared with unselected sperm (4,770 per man, 162,210 in all), the chromosomal disomy frequencies were reduced to 0.16% from 0.52%, diploidy to 0.09% from 0.51%, and sex chromosome disomy to 0.05% from 0.27% (a 5.4-fold reduction vs. 4-fold respective increase in ICSI offspring).

Conclusion(s)

The HA sperm selection method for ICSI, which is based on a relationship between sperm receptors for zona pellucida and HA, will likely reduce the potential genetic complications and adverse public health effects of ICSI.

Key Words:  Sperm maturity , chromosomal aneuploidies , ICSI sperm selection , genetic integrity , paternal contribution

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 Supported by National Institutes of Health grants HD-19505 and OH-04061 and by the Hungarian Eötvös Fellowship (A.J.).Current affiliations: A.J., Department of Obstetrics and Gynecology, Univeristy of Debrecen, Hungary; E.D. and A.R., Department of Obstetrics and Gynecology, University of Torino, Italy; S.C., Department of Histology and Embryology, Akdeniz University, Antalya, Turkey; E.K., Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas; D.W., Hospital for Cancer Care and Research, University of Nevada Las Vegas.

PII: S0015-0282(05)03327-3

doi:10.1016/j.fertnstert.2005.05.068

Refers to erratum:

  • Errata for “Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidies” (Jakab et al.)

    Fertility and Sterility August 2006 (Vol. 86, Issue 2, Page 498)

Fertility and Sterility
Volume 84, Issue 6 , Pages 1665-1673, December 2005

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