Fertility and Sterility
Volume 85, Issue 2 , Pages 474-480 , February 2006

Apoptosis of cultured granulosa-lutein cells is reduced by insulin-like growth factor I and may correlate with embryo fragmentation and pregnancy rate

  • Eija Bencomo, M.Sc.

      Affiliations

    • Laboratorio de Biología del Desarrollo, Departamento de Bioquímica y Biología Molecular, Universidad de la Laguna
    • ,
  • Rosa Pérez, M.Sc.

      Affiliations

    • Laboratorio de Biología del Desarrollo, Departamento de Bioquímica y Biología Molecular, Universidad de la Laguna
    • ,
  • Mari-Francis Arteaga, Ph.D.

      Affiliations

    • Laboratorio de Biología del Desarrollo, Departamento de Bioquímica y Biología Molecular, Universidad de la Laguna
    • ,
  • Elisa Acosta, M.Sc.

      Affiliations

    • Laboratorio de Biología del Desarrollo, Departamento de Bioquímica y Biología Molecular, Universidad de la Laguna
    • ,
  • Oscar Peña, M.Sc.

      Affiliations

    • Laboratorio de Biología del Desarrollo, Departamento de Bioquímica y Biología Molecular, Universidad de la Laguna
    • ,
  • Lourdes Lopez, M.D.

      Affiliations

    • Centro de Asistencia a la Reproducción Humana de Canarias, La Laguna, Tenerife, Spain
    • ,
  • Julio Avila, Ph.D.

      Affiliations

    • Laboratorio de Biología del Desarrollo, Departamento de Bioquímica y Biología Molecular, Universidad de la Laguna
    • ,
  • Angela Palumbo, M.D., Ph.D.

      Affiliations

    • Centro de Asistencia a la Reproducción Humana de Canarias, La Laguna, Tenerife, Spain
    • Corresponding Author InformationReprint requests: Angela Palumbo, M.D., Ph.D., Centro de Asistencia a la Reproducción Humana de Canarias, C/Doctor Zamenhof 14, E-38204 La Laguna, Tenerife, Spain (FAX: 34-922-63-28-79).

Received 9 February 2005 ,Revised 8 August 2005 ,Accepted 8 August 2005.

References 

  1. Jolly PD , Tisdall DJ , De’ath G , Heath DA , Lun S , Hudson N , et al.   Granulosa cell apoptosis, aromatase activity, cyclic adenosine 3′,5′-monophosphate response to gonadotropins, and follicular fluid steroid levels during spontaneous and induced follicular atresia in ewes . Biol Reprod . 1997;56:830–836
  2. Yuan W , Giudice LC . Programmed cell death in human ovary is a function of follicle and corpus luteum status . J Clin Endocrinol Metab . 1997;82:3148–3155
  3. Palumbo A , Yeh J . In situ localization of apoptosis in the rat ovary during follicular atresia . Biol Reprod . 1994;51:888–895
  4. Vaskivuo TE , Anttonen M , Herve R , Billig H , Dorland M , te Velde ER , et al.   Survival of human ovarian follicles from fetal to adult life (apoptosis, apoptosis-related proteins, and transcription factor GATA-4) . J Clin Endocrinol Metab . 2001;86:3421–3429
  5. Tilly JL , Kowalski KI , Johnson AL , Hsueh AJW . Involvement of apoptosis in ovarian follicular atresia and postovulatory regression . Endocrinology . 1991;129:2799–2801
  6. Hughes FM , Gorospe WC . Biochemical identification of apoptosis (programmed cell death) in granulosa cells (evidence for a potential mechanism underlying follicular atresia) . Endocrinology . 1991;129:2415–2422
  7. Nakahara K , Saito H , Saito T , Ito M , Ohta N , Takahashi T , et al.   The incidence of apoptotic bodies in membrana granulosa can predict prognosis of ova from patients participating in in vitro fertilization programs . Fertil Steril . 1997;68:312–317
  8. Nakahara K , Saito H , Saito T , Ito M , Ohta N , Sakai N , et al.   Incidence of apoptotic bodies in membrana granulosa of the patients participating in an in vitro fertilization program . Fertil Steril . 1997;67:302–308
  9. Oosterhuis GJE , Michgelsen HW , Lambalk CB , Shoemaker J , Vermes I . Apoptotic cell death in human granulosa-lutein cells (a possible indicator of in vitro fertilization outcome) . Fertil Steril . 1998;70:747–749
  10. Suh CS , Jee BC , Choi YM , Kim JG , Moon SY , Kim SH . Prognostic implication of apoptosis in human luteinized granulosa cells during IVF-ET . J Assist Reprod Genet . 2002;19:209–214
  11. Sifer C , Bénifla J-L , Bringuier A-F , Porcher R , Blanc-Layrac G , Madélénat P , et al.   Could induced apoptosis of human granulosa cells predict in vitro fertilization-embryo transfer outcome? A preliminary study of 25 women . Eur J Obstet Gynecol Reprod Biol . 2002;103:150–153
  12. Poretsky L , Cataldo NA , Rosenwaks Z , Giudice LC . The insulin-related ovarian regulatory system in health and disease . Endocr Rev . 1999;20:535–582
  13. Duleba AJ , Spacynski RZ , Tilly JL , Olive DL . Insulin and insulin-like growth factors protect ovarian theca-interstitial cells from apoptosis . J Soc Gynecol Invest . 1998;5:102A–103A
  14. Johnson AL , Bridgham JT , Swenson JA . Activation of the Akt-protein kinase B signaling pathway is associated with granulosa cell survival . Biol Reprod . 2001;64:1566–1574
  15. Guthrie HD , Garrett WM , Cooper BS . Follicle-stimulating hormone and insulin-like growth factor I attenuate apoptosis in cultured porcine granulosa cells . Biol Reprod . 1998;58:390–396
  16. Bergh C , Carlsson B , Alsson JH , Billig H , Hillensjo T . Effects of insulin-like growth factor I and growth hormone in cultured human granulosa cells . Ann NY Acad Sci . 1991;626:169–176
  17. Willis D , Mason H , Gilling-Smith C , Franks S . Modulation by insulin of follicle-stimulating hormone and luteinizing hormone actions in human granulosa cells of normal and polycystic ovaries . J Clin Endocrinol Metab . 1996;81:302–309
  18. Seifer DB , Garnider AC , Ferreira KA , Peluso JJ . Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization . Fertil Steril . 1996;66:593–598
  19. Duleba AJ , Spaczynski RZ , Olive DL . Insulin and insulin-like growth factor I stimulate the proliferation of human ovarian theca-interstitial cells . Fertil Steril . 1998;69:335–340

 Supported by grant PI2001/002 from Gobierno de Canarias, S/C de Tenerife, Spain; by PI030667 from Fondo Investigaciones Sanitarias, Madrid, Spain, and by an educational grant (27303, to E.B.) from Fundación Salud 2000, Serono, Madrid, Spain.

PII: S0015-0282(05)03847-1

doi: 10.1016/j.fertnstert.2005.08.014

Fertility and Sterility
Volume 85, Issue 2 , Pages 474-480 , February 2006

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