Fertility and Sterility
Volume 85, Issue 5 , Pages 1415-1419 , May 2006

Use of a medium buffered with N-hydroxyethylpiperazine-N-ethanesulfonate (HEPES) in intracytoplasmic sperm injection procedures is detrimental to the outcome of in vitro fertlization

  • Francesco Morgia, B.S.

      Affiliations

    • Bioroma, Center of Assisted Reproduction, “Paideia” Hospital, Rome, Italy
    • ,
  • Monica Torti, B.S.

      Affiliations

    • Bioroma, Center of Assisted Reproduction, “Paideia” Hospital, Rome, Italy
    • ,
  • Monica Montigiani, B.S.

      Affiliations

    • Bioroma, Center of Assisted Reproduction, “Paideia” Hospital, Rome, Italy
    • ,
  • Claudio Piscitelli, M.D.

      Affiliations

    • Bioroma, Center of Assisted Reproduction, “Paideia” Hospital, Rome, Italy
    • ,
  • Annalise Giallonardo, M.D.

      Affiliations

    • Bioroma, Center of Assisted Reproduction, “Paideia” Hospital, Rome, Italy
    • ,
  • Mauro Schimberni, M.D.

      Affiliations

    • Bioroma, Center of Assisted Reproduction, “Paideia” Hospital, Rome, Italy
    • ,
  • Pierluigi Giannini, M.D.

      Affiliations

    • Bioroma, Center of Assisted Reproduction, “Paideia” Hospital, Rome, Italy
    • ,
  • Marco Sbracia, M.D.

      Affiliations

    • Center of Endocrinology and Reproductive Medicine, Rome, Italy
    • Corresponding Author InformationReprint requests: Marco Sbracia, M.D., Center for Endocrinology and Reproductive Medicine, Via Carlo Porta 10, 00153, Rome, Italy (FAX: 39-06-5880096)

Received 23 June 2005 ,Revised 25 October 2005 ,Accepted 25 October 2005.

References 

  1. Gardner DK , Vella P , Lane M , Wagley L , Schlenker T , Schoolcraft WB . Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers . Fertil Steril . 1998;69:84–88
  2. Gardner DK . Development of serum-free media for the culture and transfer of human blastocysts . Hum Reprod . 1998;13(Suppl 4):218–225
  3. Van Langendonckt A , Demylle D , Wyns C , Nisolle M , Donnez J . Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos (a prospective, randomized, comparative study) . Fertil Steril . 2001;76:1023–1031
  4. Cooke S , Quinn P , Kime L , Ayres C , Tyler JR , Driscoll GL . Improvment in early human embryo development using new formulation sequential stage-specific culture media . Fertil Steril . 2002;78:1254–1260
  5. Iwasaki T , Kimura E , Totsukawa K . Studies on a chemically defined medium for in vitro culture of in vitro matured and fertilized porcine oocytes . Theriogenology . 1999;51:709–720
  6. Escriba MJ , Silvestre MA , Saeed AM , Garcia-Ximenez F . Comparison of the effect of two different handling media on rabbit zygote developmental ability . Reprod Nutr Dev . 2001;41:181–186
  7. Li M , Farley RA , Lester HA . Voltage-dependent transient currents of human and rat 5-HT transporters (SERT) are blocked by HEPES and ion channel ligands . FEBS Lett . 2002;513:247–252
  8. Lee MA , Storey BT . Bicarbonate is essential for fertilization of mouse eggs (mouse sperm require it to undergo the acrosome reaction) . Biol Reprod . 1986;34:349–356
  9. Bhattacharyya A , Yanagimachi R . Synthetic organic pH buffers can support fertilization of guinea pig eggs, but not as efficiently as bicarbonate buffer . Gamete Res . 1988;19:123–129
  10. Ali J , Whitten WK , Shelton JN . Effect of culture systems on mouse early embryo development . Hum Reprod . 1993;8:1110–1114
  11. Suzuki K , Ebihara M , Nagai T , Clarke NG , Harrison RA . Importance of bicarbonate/CO2 for fertilization of pig oocytes in vitro, and synergism with caffeine . Reprod Fertil Dev . 1994;6:221–227
  12. Pollard JW , Plante C , Leibo SP . Comparison of development of pig zygotes and embryos in simple and complex culture media . J Reprod Fertil . 1995;103:331–337
  13. Downs SM , Mastropolo AM . Culture conditions affect meiotic regulation in cumulus cell–enclosed mouse oocytes . Mol Reprod Dev . 1997;46:551–566
  14. Hamaguchi MS , Watanabe K , Hamaguchi Y . Regulation of intracellular pH in sea urchin eggs by medium containing both weak acid and base . Cell Struct Funct . 1997;22:387–398
  15. Quinn P , Warnes GM , Kerin JF , Kirby C . Culture factors in relation to the success of human in vitro fertilization and embryo transfer . Fertil Steril . 1984;41:202–209
  16. Palermo G , Joris H , Devroey P , Van Starteghen AL . Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte . Lancet . 1992;340:17–18
  17. Picard A , Doree M . Intracellular microinjection of alkaline buffers reversibly inhibits the initial phase of hormone action in meiosis reinitiation of starfish oocytes . Dev Biol . 1983;97:184–190
  18. Veeck LL . An atlas of human gametes and conceptus . London (UK): Parthenon Publishing; 1999;
  19. Keskintepe L , Brackett BG . In vitro developmental competence of in vitro–matured bovine oocytes fertilized and cultured in completely defined media . Biol Reprod . 1996;55:333–339
  20. Zigler JS , Lepe-Zuniga JL , Vistica B , Gery I . Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium . In Vitro Cell Dev Biol . 1985;21:282–287
  21. Bowman CM , Berger EM , Butler EN , Toth KM , Repine JE . HEPES may stimulate cultured endothelial cells to make growth-retarding oxygen metabolites . In Vitro Cell Dev Biol . 1985;21:140–142
  22. Mahadevan MM , Fleetham J , Church RB , Taylor PJ . Growth of mouse embryos in bicarbonate media buffered by carbon dioxide, HEPES, or phosphate . J In Vitro Fert Embryo Transf . 1986;3:304–308

PII: S0015-0282(06)00094-X

doi: 10.1016/j.fertnstert.2005.10.050

Fertility and Sterility
Volume 85, Issue 5 , Pages 1415-1419 , May 2006

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