Fertility and Sterility
Volume 86, Issue 4, Supplement , Pages 1137-1145 , October 2006

Serum replacement with a growth factor–free synthetic substance in culture medium contributes to effective establishment of mouse embryonic stem cells of various origins

  • Seung Tae Lee, Ph.D.

      Affiliations

    • Department of Food and Animal Biotechnology, Seoul National University, Seoul, Korea
    • ,
  • Se Woong Oh, D.V.M., M.S.

      Affiliations

    • College of Veterinary Medicine, Seoul National University, Seoul, Korea
    • ,
  • Dae Yong Kim, D.V.M., Ph.D.

      Affiliations

    • College of Veterinary Medicine, Seoul National University, Seoul, Korea
    • ,
  • Jae Yong Han, Ph.D.

      Affiliations

    • Department of Food and Animal Biotechnology, Seoul National University, Seoul, Korea
    • ,
  • Shin Yong Moon, M.D., Ph.D.

      Affiliations

    • Stem Cell Research Center, Seoul National University, Seoul, Korea
    • Medical Research Center, College of Medicine, Seoul National University, Seoul, Korea
    • ,
  • Jeong Mook Lim, D.V.M., Ph.D.

      Affiliations

    • Department of Food and Animal Biotechnology, Seoul National University, Seoul, Korea
    • Stem Cell Research Center, Seoul National University, Seoul, Korea
    • Corresponding Author InformationReprint requests: Jeong Mook Lim, D.V.M., Ph.D., Department of Food and Animal Biotechnology, Laboratory of Gamete and Stem Cell Biotechnology, Seoul National University, Building 200-#4223, Sillim-9 Dong, Seoul 151-921, Korea (FAX: 822-874-2555).

Received 5 October 2005 ,Revised 6 January 2006 ,Accepted 6 January 2006.

References 

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  9. Kawase E , Yamazaki Y , Yagi T , Yanagimachi R , Pedersen RA . Mouse embryonic stem (ES) cell lines established from neuronal cell-derived cloned blastocysts . Genesis . 2000;28:156–163
  10. Horii T , Nagao Y , Tokunaga T , Imai H . Serum-free culture of murine primordial germ cells and embryonic germ cells . Theriogenology . 2003;59:1257–1264
  11. Baharvand H , Matthaei KI . Culture condition difference for establishment of new embryonic stem cell lines from the C57BL/6 and BALB/c mouse strains . In Vitro Cell Dev Biol Anim . 2004;40:76–81
  12. Kawase E , Suemoi H , Takahashi N , Okazaki K , Hashimoto K , Nakatsuji N . Strain difference in establishment of mouse embryonic stem (ES) cell lines . Int J Dev Biol . 1994;38:385–390
  13. Meng GL , Tang FC , Shang KG , Xue YF . Comparison of the method establishing embryonic stem cell lines from five different mouse strains . Yi Chuan Xue Bao . 2003;30:933–942
  14. Baharvand H , Ashtiani SK , Valojerdi MR , Shahverdi A , Taee A , Sabour D . Establishment and in vitro differentiation of a new embryonic stem cell line from human blastocyst . Differentiation . 2004;72:224–229
  15. Cho M , Jang M , Lee EJ , Han JY , Lim JM . An alternative method of deriving embryonic stem cell-like clones by aggregation of diploid cells with tetraploid embryos . Fertil Steril . 2006;85:1103–1110
  16. Han MS , Niwa K . Effects of BSA and fetal bovine serum in culture medium on development of rat embryos . J Reprod Dev . 2003;49:235–242

 Supported by a grant from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Gwacheon, Korea (SC-2170). The authors also acknowledge a graduate fellowship provided by the Korean Ministry of Education through the Brain Korea 21 project, Seoul, Korea.

PII: S0015-0282(06)01078-8

doi: 10.1016/j.fertnstert.2006.01.056

Fertility and Sterility
Volume 86, Issue 4, Supplement , Pages 1137-1145 , October 2006

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