In vivo spermatotoxic effect of chromium as reflected in the epididymal epithelial principal cells, basal cells, and intraepithelial macrophages of a nonhuman primate (Macaca radiata Geoffroy)

Objective

To understand, through a simulation experiment in a nonhuman primate model, the potential in vivo spermatotoxic toxic effect of hexavalent chromium (CrVI) in men who are occupationally or environmentally exposed to it.

Design

Controlled laboratory study.

Setting

Research laboratory in a department of endocrinology in a university in India.

Animal(s)

Male bonnet monkey, Macaca radiata Geoffroy.

Intervention(s)

Monkeys were exposed ad libitum to 100, 200, and 400 ppm CrVI, dissolved in drinking water, for a chronic period of 180 days.

Main Outcome Measure(s)

Examination of epididymis with a transmission electron microscope and assessment of the effect of CrVI in terms of accumulation of sperm-derived lipofuscin (LF) material in the principal cells, basal cells, and intraepithelial macrophages of the epithelium.

Result(s)

The abundance of basal cells and intraepithelial macrophages and the content of LF material in these cell types increased. The principal cells phagocytosed from the lumen the dead sperm resulting from CrVI exposure and processed them partially into LF material, which was acquired by the basal cells and intraepithelial macrophages and processed further. The LF material–laden basal cells and intraepithelial macrophages appeared to leave the epithelium, accompanied by recruitment of fresh basal cells and intraepithelial macrophages.

Conclusion(s)

Occupational or environmental exposure to CrVI, as would occur in the tannery, soap, and other industries in developing and underdeveloped countries, can be toxic in vivo to spermatozoa.

Key Words:  Basal cell , chromium , epididymis , intraepithelial macrophage , lipofuscin material , principal cell , spermatotoxicity