Fertility and Sterility
Volume 87, Issue 5 , Pages 1033-1040 , May 2007

Oocyte karyotyping by comparative genomic hybrydization provides a highly reliable method for selecting “competent” embryos, markedly improving in vitro fertilization outcome: a multiphase study

  • Geoffrey Sher, M.D.

      Affiliations

    • Sher Institute for Reproductive Medicine, Las Vegas, Nevada
    • Department of Obstetrics and Gynecology, University of Nevada School of Medicine, Reno
    • Corresponding Author InformationReprint requests: Geoffrey Sher, M.D., Sher Institute for Reproductive Medicine, Las Vegas, 3121 S. Maryland Parkway, Suite 300, Las Vegas, NV 89109 (FAX: 702-892-9666).
    • ,
  • Levent Keskintepe, Ph.D.

      Affiliations

    • Sher Institute for Reproductive Medicine, Las Vegas, Nevada
    • ,
  • Meral Keskintepe, Ph.D.

      Affiliations

    • ReproCure Reproductive Genetics, Las Vegas, Nevada
    • ,
  • Mike Ginsburg, M.S.

      Affiliations

    • ReproCure Reproductive Genetics, Las Vegas, Nevada
    • ,
  • Ghanima Maassarani, Dr. Med.

      Affiliations

    • Sher Institute for Reproductive Medicine, Las Vegas, Nevada
    • ,
  • Tahsin Yakut, M.D.

      Affiliations

    • School of Medicine, University of Uludag, Bursa
    • ,
  • Volkan Baltaci, M.D.

      Affiliations

    • Gen-ART, Ankara, Turkey
    • ,
  • Dirk Kotze, M.S.

      Affiliations

    • Sher Institute for Reproductive Medicine, Las Vegas, Nevada
    • ,
  • Evrim Unsal, B.S.

      Affiliations

    • Gen-ART, Ankara, Turkey

Received 16 June 2006 ,Revised 18 August 2006 ,Accepted 18 August 2006.

References 

  1. Mikamo K. Anatomic and chromosomal anomalies in spontaneous abortion (Possible correlation with over-ripeness of oocytes). Am J Obstet Gynecol. 1970;106:243–254
  2. Baranov VS, Dyban AP, Chebotar NA. Preimplantation development of mouse embryos with monosomy of autosome No. 17. Ontogenez. 1980;11:148–159
  3. Jagiello G, Fang JS. A pachytene map of the mouse oocyte. Chromosoma. 1980;77:113–121
  4. Clement-Sengewald A, Buchholz T, Schutze K. Laser microdissection as a new approach to pre-fertilization genetic diagnosis. Pathobiology. 2000;68:232–236
  5. Martini E, Flaherty SP, Swann NJ, Matthews CD, Ramaekers FC, Geraedts JP. FISH analysis of six chromosomes in unfertilized human oocytes after polar body removal. J Assist Reprod Genet. 2000;17:276–283
  6. Gitlin SA, Gibbons WE, Gosden RG. Oocyte biology and genetics revelations from polar bodies. Reprod Biomed Online. 2003;6:403–409
  7. Le Caignec C, Spits C, Sermon K, De Rycke M, Thienpont B, Debrock S, et al. Single-cell chromosomal imbalances detection by array CGH. Nucleic Acids Res. 2006;34:e68
  8. Wilton L. Preimplantation genetic diagnosis and chromosome analysis of blastomeres using comparative genomic hybridization. Hum Reprod Update. 2005;11:33–41
  9. Wells D, Levy B. Cytogenetics in reproductive medicine: the contribution of comparative genomic hybridization (CGH). Bioassays. 2003;25:289–300
  10. Murthy SK, Demetrick DJ. New approaches to fluorescence in situ hybridization. Methods Mol Biol. 2006;319:237–259
  11. Chen CP, Lin SP, Lin CC, Li YC, Hsieh LJ, Huang JK, et al. Spectral karyotyping and fluorescence in situ hybridization analysis of de novo partial trisomy 7p (7p21.2->pter) and partial monosomy 12q (12q24.33->qter). Genet Couns. 2006;17:57–63
  12. Pellestor F, Andreo B, Anahory T, Hamamah S. The occurrence of aneuploidy in human: lessons from the cytogenetic studies of human oocytes. Eur J Med Genet. 2006;49:103–116
  13. Pellestor F, Andreo B, Anahory T, Dechaud H, Hedon B, Hamamah S. The cytogenetics of human oocytes: 40 years of progress. Gynecol Obstet Fertil. 2005;33:283–292
  14. Fragouli E, Wells D, Whalley KM, Mills JA, Faed MJ, Delhanty JD. Increased susceptibility to maternal aneuploidy demonstrated by comparative genomic hybridization analysis of human MII oocytes and first polar bodies. Cytogenet Genome Res. 2006;114:30–38
  15. Wang H, Hoog C. Structural damage to meiotic chromosomes impairs DNA recombination and checkpoint control in mammalian oocytes. J Cell Biol. 2006;173:485–495
  16. Lightfoot DA, Kouznetsova A, Mahdy E, Wilbertz J, Hoog C. The fate of mosaic aneuploid embryos during mouse development. Dev Biol. 2006;289:384–394
  17. Delhanty JD. Mechanisms of aneuploidy induction in human oogenesis and early embryogenesis. Cytogenet Genome Res. 2005;111:237–244
  18. Baart EB, Martini E, van den Berg I, Macklon NS, Galjaard RJ, Fauser BC, et al. Preimplantation genetic screening reveals a high incidence of aneuploidy and mosaicism in embryos from young women undergoing IVF. Hum Reprod. 2006;21:223–233
  19. Sher G, Fisch JD. Effect of vaginal sildenafil on the outcome of in vitro fertilization (IVF) after multiple IVF failures attributed to poor endometrial development. Fertil Steril. 2002;78:1073–1076
  20. Sher G, Keskintepe L, Nouriani M, Roussev R, Batzofin J. Expression of sHLA-G in supernatants of individually cultured 46-h embryos: a potentially valuable indicator of “embryo competency” and IVF outcome. Reprod Biomed Online. 2004;9:74–78
  21. Selva J, Bergere M, Molina-Gomes D, Hammoud I, Lombroso R, Vialard F. Contribution of first polar body analysis to understanding of human aneuploidy mechanism. Bull Acad Natl Med. 2005;189:1761–1772
  22. Montag M, Schimming T, van der Ven H. Spindle imaging in human oocytes: the impact of the meiotic cell cycle. Reprod Biomed Online. 2006;12:442–446
  23. Ebner T, Moser M, Tews G. Is oocyte morphology prognostic of embryo developmental potential after ICSI?. Reprod Biomed Online. 2006;12:507–512
  24. Hewitson L, Simerly C, Schatten G. Cytoskeletal aspects of assisted fertilization. Semin Reprod Med. 2000;182:151–159
  25. Murthy SK, Demetrick DJ. New approaches to fluorescence in situ hybridization. Methods Mol Biol. 2006;319:237–259
  26. Wilton L, Voullaire L, Sargeant P, Williamson R, McBain J. Preimplantation aneuploidy screening using comparative genomic hybridization or fluorescence in situ hybridization of embryos from patients with recurrent implantation failure. Fertil Steril. 2003;80:860–868
  27. Wells D, Escudero T, Levy B, Hirschhorn K, Delhanty JD, Munne S. First clinical application of comparative genomic hybridization and polar body testing for preimplantation genetic diagnosis of aneuploidy. Fertil Steril. 2002;78:543–549
  28. Voullaire L, Wilton L, McBain J, Callaghan T, Williamson R. Chromosome abnormalities identified by comparative genomic hybridization in embryos from women with repeated implantation failure. Mol Hum Reprod. 2002;8:1035–1041
  29. Gutiérrez-Mateo C, Benet J, Wells D, Colls P, Bermudez MG, Sanchez-Garcia JF, et al. Aneuploidy study of human oocytes first polar body comparative genomic hybridization and metaphase II fluorescence in situ hybridization analysis. Hum Reprod. 2004;19:2859–2868
  30. Gutiérrez-Mateo C, Benet J, Wells D, Colls P, Bermudez MG, Sanchez-Garcia JF, et al. Aneuploidy study of human oocytes first polar body comparative genomic hybridization and metaphase II fluorescence in situ hybridization analysis. Hum Reprod. 2004;19:2859–2868
  31. Warburton D, Kinney A. Chromosomal differences in susceptibility to meiotic aneuploidy. Environ Mol Mutagen. 1996;28:237–247
  32. Lamb NE, Sherman SL, Hassold TJ. Effect of meiotic recombination on the production of aneuploid gametes in humans. Cytogenet Genome Res. 2005;111:250–255
  33. Munne S. Chromosome abnormalities and their relationship to morphology and development of human embryos. Reprod Biomed Online. 2006;12:234–253
  34. Takahashi K, Mukaida T, Goto T, Oka C. Perinatal outcome of blastocyst transfer with vitrification using cryoloop: a 4-year follow-up study. Fertil Steril. 2005;84:88–92
  35. Hanson C, Caisander G. Human embryonic stem cells and chromosome stability. APMIS. 2005;113:751–755

PII: S0015-0282(06)04447-5

doi: 10.1016/j.fertnstert.2006.08.108

Fertility and Sterility
Volume 87, Issue 5 , Pages 1033-1040 , May 2007

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