Fertility and Sterility
Volume 88, Issue 2 , Pages 379-382, August 2007

Expression of various CDC25B isoforms in human spermatozoa

  • Yen-Ni Teng, Ph.D.

      Affiliations

    • Department of Biotechnology, Chia Nan University of Pharmacy and Science, National Cheng Kung University, College of Medicine, Tainan, Taiwan
    • ,
  • Chia-Ling Chung, M.Sc.

      Affiliations

    • Division of Genetics, Department of Obstetrics and Gynecology, National Cheng Kung University, College of Medicine, Tainan, Taiwan
    • ,
  • Yung-Ming Lin, M.D.

      Affiliations

    • Department of Urology, National Cheng Kung University, College of Medicine, Tainan, Taiwan
    • ,
  • Hsien-An Pan, M.D.

      Affiliations

    • Division of Genetics, Department of Obstetrics and Gynecology, National Cheng Kung University, College of Medicine, Tainan, Taiwan
    • ,
  • Rui-Wen Liao, M.Sc.

      Affiliations

    • Division of Genetics, Department of Obstetrics and Gynecology, National Cheng Kung University, College of Medicine, Tainan, Taiwan
    • ,
  • Pao-Lin Kuo, M.D.

      Affiliations

    • Division of Genetics, Department of Obstetrics and Gynecology, National Cheng Kung University, College of Medicine, Tainan, Taiwan
    • Corresponding Author InformationReprint requests: Pao-Lin Kuo, M.D., Division of Genetics, Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, 138 Sheng-Li Road, Tainan 704, Taiwan (FAX: 886-6-276-6185).

Received 23 May 2006; received in revised form 7 November 2006; accepted 21 November 2006. published online 05 March 2007.

Objective

To explore the role of CDC25B protein in postmeiotic germ cells.

Design

In vitro experiment.

Setting

University-based reproductive genetics laboratory.

Patient(s)

Fertile and infertile volunteers.

Intervention(s)

Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B.

Main Outcome Measure

Expression profiling of CDC25B in human spermatozoa.

Result(s)

Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia.

Conclusion(s)

The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.

Key Words: Human spermatozoa, CDC25B, RT-PCR, immunofluorescence staining, real-time RT-PCR

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 Supported by grants NSC 94-2314-B-006-075, NSC 94-2314-B-006-076, and NSC 94-2320-B-041-012 from the National Science Council of Taiwan, Taipei, Taiwan.

PII: S0015-0282(06)04663-2

doi:10.1016/j.fertnstert.2006.11.186

Fertility and Sterility
Volume 88, Issue 2 , Pages 379-382, August 2007

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