Fertility and Sterility
Volume 88, Issue 6 , Pages 1534-1540, December 2007

Characterization of WNT7A expression in human endometrium and endometriotic lesions

  • Regine Gaetje, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Johann Wolfgang Goethe-University, Frankfurt, Germany
    • Corresponding Author InformationReprint requests: Regine Gaetje, M.D., Department of Obstetrics and Gynecology, Johann Wolfgang Goethe-University, Theodor Stern-Kai 7, 60596 Frankfurt, Germany (FAX: +49-69-6301-7034).
    • ,
  • Uwe Holtrich, Ph.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Johann Wolfgang Goethe-University, Frankfurt, Germany
    • ,
  • Thomas Karn, Ph.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Johann Wolfgang Goethe-University, Frankfurt, Germany
    • ,
  • Eva Cikrit

      Affiliations

    • Department of Obstetrics and Gynecology, Johann Wolfgang Goethe-University, Frankfurt, Germany
    • ,
  • Knut Engels, M.D.

      Affiliations

    • Department of Pathology, Johann Wolfgang Goethe-University, Frankfurt, Germany
    • ,
  • Achim Rody, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Johann Wolfgang Goethe-University, Frankfurt, Germany
    • ,
  • Manfred Kaufmann, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Johann Wolfgang Goethe-University, Frankfurt, Germany

Received 3 November 2006; received in revised form 26 January 2007; accepted 26 January 2007. published online 22 June 2007.

Objective

To characterize the expression of WNT7A in human eutopic and ectopic endometrium.

Design

Experimental study using real-time polymerase chain reaction, laser microdissection, in situ hybridization, and immunofluorescence.

Setting

University-based laboratory.

Patient(s)

Patients with and without endometriosis undergoing surgery for benign indications.

Intervention(s)

None.

Main Outcome Measure(s)

Relative expression values compared with housekeeping genes using real-time polymerase chain reaction. Detection of positive cells by immunofluorescence and in situ hybridization.

Result(s)

In endometriosis, statistically significant higher WNT7A mRNA expression was observed compared with eutopic endometrium. Expression of WNT7A was found in the luminal and glandular epithelial cells as well as stroma cells in endometrium and endometriosis by immunofluorescence, in situ hybridization, and polymerase chain reaction of laser microdissected tissue.

Conclusion(s)

The results of the present study suggest that WNT7A plays a role in the pathophysiology of endometriosis.

Key Words: WNT wingless-type MMTV integration site family, endometrium, endometriosis

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 Supported by grants from Deutsche Krebshilfe, Bonn Margarete Bonifer-Stiftung, Bad Soden, BANSS-Stiftung, Biedenkopf, and Dr. Robert Pfleger-Stiftung, Bamberg.

PII: S0015-0282(07)00276-2

doi:10.1016/j.fertnstert.2007.01.128

Fertility and Sterility
Volume 88, Issue 6 , Pages 1534-1540, December 2007

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