Fertility and Sterility
Volume 89, Issue 2 , Pages 358-363 , February 2008

Human blastocyst morphological quality is significantly improved in embryos classified as fast on day 3 (≥10 cells), bringing into question current embryological dogma

  • Martha Luna, M.D.

      Affiliations

    • Mount Sinai School of Medicine, Department of Obstetrics and Gynecology, Department of Reproductive Endocrinology and Infertility, New York, New York
    • Reproductive Medicine Associates of New York, New York, New York
  • ,
  • Alan B. Copperman, M.D.

      Affiliations

    • Mount Sinai School of Medicine, Department of Obstetrics and Gynecology, Department of Reproductive Endocrinology and Infertility, New York, New York
    • Reproductive Medicine Associates of New York, New York, New York
    • Corresponding Author InformationReprint requests: Alan B. Copperman, M.D., Department of Obstetrics and Gynecology, Department of Reproductive Endocrinology and Infertility, Mount Sinai Medical Center, Reproductive Medicine Associates of New York, 635 Madison Ave., 10th Floor, New York, NY 10022 (FAX: 212-756-5770).
  • ,
  • Marlena Duke, M.Sc.

      Affiliations

    • Mount Sinai School of Medicine, Department of Obstetrics and Gynecology, Department of Reproductive Endocrinology and Infertility, New York, New York
    • Reproductive Medicine Associates of New York, New York, New York
  • ,
  • Diego Ezcurra, D.V.M.

      Affiliations

    • EMD Serono, Rockland, Massachusetts
  • ,
  • Benjamin Sandler, M.D.

      Affiliations

    • Mount Sinai School of Medicine, Department of Obstetrics and Gynecology, Department of Reproductive Endocrinology and Infertility, New York, New York
    • Reproductive Medicine Associates of New York, New York, New York
  • ,
  • Jason Barritt, Ph.D.

      Affiliations

    • Mount Sinai School of Medicine, Department of Obstetrics and Gynecology, Department of Reproductive Endocrinology and Infertility, New York, New York
    • Reproductive Medicine Associates of New York, New York, New York

Received 10 October 2006 ,Revised 5 March 2007 ,Accepted 7 March 2007.

References 

  1. Hardarson T, Hanson C, Sjögren A, Lundin K. Human embryos with unevenly sized blastomeres have lower pregnancy and implantation rates: indications for aneuploidy and multinucleation. Hum Reprod. 2001;16:313–318
  2. Van Royen E, Mangelschots K, Vercruyssen M, De Neubourg D, Valkenburg M, Ryckaert G, et al. Multinucleation in cleavage stage embryos. Hum Reprod. 2003;18:1062–1069
  3. Ziebe S, Peterson K, Lindenberg S, Anderson AG, Gabrielsen A, Andersen A. Embryo morphology or cleavage stage: how to select the best embryos for transfer after in vitro fertilization. Hum Reprod. 1997;12:1545–1549
  4. Alikani M, Calderon G, Tomkin G, Garrisi J, Kokott M, Cohen J. Cleavage anomalies in early human embryos and survival after prolonged culture in vitro. Hum Reprod. 2000;15:2634–2643
  5. McLaren A, Bowman P. Genetic effects on the timing of early development in the mouse. J Embryol Exp Morphol. 1973;30:491–498
  6. Grisart B, Massip A, Dessy F. Cinematographic analysis of bovine embryo development in serum free oviduct-conditioned medium. J Reprod Fertil. 1994;101:257–264
  7. Lonergan P, O'Kearney-Flynn M, Boland M. Effect of protein supplementation and presence of an antioxidant on the development of bovine zygotes in synthetic oviduct fluid medium under high or low oxygen tension. Theriogenology. 1999;51:1565–1576
  8. Bavister B, Boatman D, Leibfried M. Fertilization and cleavage of rhesus monkey oocytes in vitro. Biol Reprod. 1983;28:983–999
  9. Bernardi ML, Delouis C. Sex related differences in the developmental rate of in vitro matured/in vitro fertilized ovine embryos. Hum Reprod. 1996;11:621–626
  10. Bos-Mikich A, Mattos A, Ferrari A. Early cleavage of human embryos: an effective method for predicting successful IVF/ICSI outcome. Hum Reprod. 2001;16:2658–2661
  11. Fenwick J, Plattteau P, Murdoch A, Herbert M. Time from insemination to first cleavage predicts developmental competence of human preimplantation embryos in vitro. Hum Reprod. 2002;17:407–412
  12. Lundin K, Bergh C, Hardarson T. Early embryo cleavage is a strong indicator of embryo quality in human IVF. Hum Reprod. 2001;16:2652–2657
  13. Fisch J, Sher G, Adamowicz M, Keskintepe L. The graduated embryo score predicts the outcome of assisted reproductive technologies better than a single day 3 evaluation and achieves results associated with blastocyst transfer from day 3 embryo transfer. Fertil Steril. 2003;80:1352–1358
  14. Harper J, Robinson F, Duffy S. Detection of fertilization in embryos with accelerated cleavage by fluorescence in situ hybridization. Hum Reprod. 1994;9:1733–1737
  15. Magli M, Gianaroli L, Munne S, Ferraretti A. Incidence of chromosomal abnormalities from a morphological normal cohort of embryos in poor prognosis patients. J Assist Reprod Genet. 1998;15:297–301
  16. Sandalinas M, Sadowy S, Alikani M, Calderon G, Cohen J, Munne S. Developmental ability of chromosomally abnormal human embryos to develop to the blastocyst stage. Hum Reprod. 2001;16:1954–1958
  17. Gardner D, Schoolcraft W. In vitro culture of human blastocyst. In:  Jansen R,  Mortimer D editor. Towards reproductive certainty: infertility and genetics beyond 1999. Carnforth: Parthenon Press; 1999;p. 378–388
  18. Edwards R, Fishel S, Cohen J. Factors influencing the success of in vitro fertilization for alleviating human infertility. J In Vitro Fertil Embryo Transfer. 1984;1:3–23
  19. Shapiro B, Richter K, Harris D. Predictive value of 72-hour blastomere cell number on blastocyst development and success of a subsequent transfer based on the degree of blastocyst development. Fertil Steril. 2000;73:582–586
  20. McKiernan S, Bavister B. Timing of development is a critical parameter for predicting successful embryogenesis. Hum Reprod. 1994;9:2123–2129
  21. Totey S, Daliri M, Rao K. Differential cleavage and developmental rates and their correlation with cell numbers and sex ratios in buffalo embryos generated in vitro. Theriogenology. 1996;45:521–533
  22. Lonergan P, Khatir H, Piumi F. Effect of time interval from insemination to first cleavage on the developmental characteristics, sex and pregnancy rates following transfer of bovine preimplantation embryos. J Reprod Fertil. 1999;117:159–167
  23. Sakkas D, Shoukir Y, Chardoennens D. Early cleavage of human embryos to the two cell stage after intracytoplasmic sperm injection as an indicator of embryo viability. Hum Reprod. 1998;13:182–187
  24. Shoukir Y, Chardonnens D, Campana A. The rate of development and time of transfer play different roles in influencing the viability of human blastocysts. Hum Reprod. 1998;13:676–681
  25. Van Montfoort A, Dumoulin J, Kester A, Evers J. Early cleavage is a valuable addition to existing embryo selection parameters: a study using single embryo transfers. Hum Reprod. 2004;19:2103–2108

PII: S0015-0282(07)00616-4

doi: 10.1016/j.fertnstert.2007.03.030

Fertility and Sterility
Volume 89, Issue 2 , Pages 358-363 , February 2008