Fertility and Sterility
Volume 89, Issue 3 , Pages 656-661 , March 2008

Proportional change of CD4+CD25+ regulatory T cells in decidua and peripheral blood in unexplained recurrent spontaneous abortion patients

  • Hui Yang, M.D., Ph.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    • ,
  • Lihua Qiu, M.D., Ph.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    • ,
  • Guangjie Chen, Ph.D.

      Affiliations

    • Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    • ,
  • Zi Ye, B.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    • ,
  • Caijun Lü, B.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    • ,
  • Qide Lin, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    • Corresponding Author InformationCorrespondence to: Qide Lin, M.D., Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 145 Shandong Mid Road, Shanghai, 200001, China (FAX: 86-21-63730455).

Received 20 September 2006 ,Revised 13 March 2007 ,Accepted 13 March 2007.

References 

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  2. Patriarca A, Piccioni V, Gigante V, Benedetto C. The use of intravenous immunoglobulin in sine causa or alloimmune recurrent spontaneous abortion (RSA). Panminerva Med. 2000;42:193–195
  3. Christiansen OB, Nybo Andersen AM, Bosch E, Daya S, Delves PJ, Hviid TV, et al. Evidence-based investigations and treatments of recurrent pregnancy loss. Fertil Steril. 2005;83:821–839
  4. Bulletti C, Flamigni C, Giacomucci E. Reproductive failure due to spontaneous abortion and recurrent miscarriage. Hum Reprod Update. 1996;2:118–136
  5. Darrasse-Jeze G, Klatzmann D, Charlotte F, Salomon BL, Cohen JL. CD4+CD25+ regulatory/suppressor T cells prevent allogeneic fetus rejection in mice. Immunol Lett. 2006;102:106–109
  6. Zenclussen AC, Gerlof K, Zenclussen ML, Sollwedel A, Bertoja AZ, Ritter T, et al. Abnormal T-cell reactivity against paternal antigens in spontaneous abortion: adoptive transfer of pregnancy-induced CD4+CD25+ T regulatory cells prevents fetal rejection in a murine abortion model. Am J Pathol. 2005;166:811–822
  7. Qiu LH, Lin QD. Study on the relationship between regulatory T cells and unexplained recurrent spontaneous abortion. Zhonghua Fu Chan Ke Za Zhi. 2004;39:816–818
  8. World Health Organization . Laboratory manual for the examination of human semen and sperm-cervical mucus interaction. New York: Cambridge University Press; 1999;
  9. Saito S, Nishikawa K, Morii T, Narita N, Enomoto M, Ichijo M. Expression of activation antigens CD69, HLA-DR, interleukin-2 receptor-alpha (IL-2R alpha) and IL-2R beta on T cells of human decidua at an early stage of pregnancy. Immunology. 1992;75:710–712
  10. Tsuda H, Michimata T, Hayakawa S, Tanebe K, Sakai M, Fujimura M, et al. A Th2 chemokine, TARC, produced by trophoblasts and endometrial gland cells, regulates the infiltration of CCR4+ T lymphocytes into human decidua at early pregnancy. Am J Reprod Immunol. 2002;48:1–8
  11. Kuniyasu Y, Takahashi T, Itoh M, Shimizu J, Toda G, Sakaguchi S. Naturally anergic and suppressive CD25(+)CD4(+) T cells as a functionally and phenotypically distinct immunoregulatory T cell subpopulation. Int Immunol. 2000;12:1145–1155
  12. Baecher-Allan C, Brown JA, Freeman GJ, Hafler DA. CD4+CD25high regulatory cells in human peripheral blood. J Immunol. 2001;167:1245–1253
  13. Cao D, Malmstrom V, Baecher-Allan C, Hafler D, Klareskog L, Trollmo C. Isolation and functional characterization of regulatory CD25brightCD4+ T cells from the target organ of patients with rheumatoid arthritis. Eur J Immunol. 2003;33:215–223
  14. Somerset DA, Zheng Y, Kilby MD, Sansom DM, Drayson MT. Normal human pregnancy is associated with an elevation in the immune suppressive CD25+CD4+ regulatory T-cell subset. Immunology. 2004;112:38–43
  15. Aluvihare VR, Kallikourdis M, Betz AG. Regulatory T cells mediate maternal tolerance to the fetus. Nat Immunol. 2004;5:266–271
  16. Sasaki Y, Sakai M, Miyazaki S, Higuma S, Shiozaki A, Saito S, et al. Decidual and peripheral blood CD4+CD25+ regulatory T cells in early pregnancy subjects and spontaneous abortion cases. Mol Hum Reprod. 2004;10:347–353
  17. Heikkinen J, Mottonen M, Alanen A, Lassila O. Phenotypic characterization of regulatory T cells in the human decidua. Clin Exp Immunol. 2004;136:373–378
  18. Tilsburg T, Roelen DL, van der Mast BJ, van Schip JJ, Kleijburg C, et al. Differential distribution of CD4(+)CD25(bright) and CD8(+)CD28(−) T-cells in decidua and maternal blood during human pregnancy. Placenta. 2006;27(Suppl A):S47–S53
  19. Vassiliadou N, Searle RF, Bulmer JN. Elevated expression of activation molecules by decidual lymphocytes in women suffering spontaneous early pregnancy loss. Hum Reprod. 1999;14:1194–1200
  20. Quack KC, Vassiliadou N, Pudney J, Anderson DJ, Hill JA. Leukocyte activation in the decidua of chromosomally normal and abnormal fetuses from women with recurrent abortion. Hum Reprod. 2001;16:949–955

 Hui Yang and Lihua Qiu contributed equally to the work and should both be considered first authors.

 Supported by the National Natural Science Foundation of China (Shanghai) (30530740/C030304).

PII: S0015-0282(07)00656-5

doi: 10.1016/j.fertnstert.2007.03.037

Fertility and Sterility
Volume 89, Issue 3 , Pages 656-661 , March 2008

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