Fertility and Sterility
Volume 90, Issue 5 , Pages 1800-1804 , November 2008

Significant decrease in sperm deoxyribonucleic acid fragmentation after varicocelectomy

  • Philip Werthman, M.D., FACS

      Affiliations

    • Center for Male Reproductive Medicine, Los Angeles, California
    • ,
  • Regina Wixon, Ph.D.

      Affiliations

    • SCSA Diagnostics, South Dakota State University, Brookings, South Dakota
    • ,
  • Kay Kasperson, B.S.

      Affiliations

    • SCSA Diagnostics, South Dakota State University, Brookings, South Dakota
    • ,
  • Donald P. Evenson, Ph.D.

      Affiliations

    • Department of Chemistry and Biochemistry, South Dakota State University, Brookings, South Dakota
    • Corresponding Author InformationReprint requests: Donald P. Evenson, Ph.D., SCSA Diagnostics, 807 32nd Ave., Brookings, SD 57006 (FAX: 605-692-9730).

Received 5 May 2006 ,Revised 21 August 2006 ,Accepted 11 September 2006.

References 

  1. Chen SS, Huang WJ, Chang LS, Wei YH. 8-hydroxy-2′-deoxyguanosine in leukocyte DNA of spermatic vein as a biomarker of oxidative stress in patients with varicocele. J Urol. 2004;172:1239–1240
  2. Saleh RA, Agarwal A, Nada EA, Mohamed H, El-Tonsy MH, Sharma RK, et al. Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. Fertil Steril. 2003;79:1597–1605
  3. Zini A, Blumenfeld A, Libman J, Willis J. Beneficial effect of microsurgical varicocelectomy on human sperm DNA integrity. Hum Reprod. 2005;20:1018–1021
  4. Evenson DP, Wixon R. Environmental toxicants cause sperm DNA fragmentation as detected by the Sperm Chromatin Structure Assay (SCSA(R)). Toxicol Appl Pharmacol. 2005;207(2 Suppl):532–537
  5. Bungum M, Humaidan P, Spano M, Jepson K, Bungum L, Giwercman A. The predictive value of sperm chromatin structure assay (SCSA®) parameters for the outcome of intrauterine insemination, IVF and ICSI. Hum Reprod. 2004;19:1401–1408
  6. Check JH, Graziano V, Cohen R, Krotec J, Check ML. Effect of an abnormal sperm chromatin structural assay (SCSA) on pregnancy outcome following (IVF) with ICSI in previous IVF failures. Arch Androl. 2005;51:121–124
  7. Spano M, Bonde J, Hjollund HI, Kolstatd HA, Cordelli E, Leter G. Sperm chromatin damage impairs human fertility. Fertil Steril. 2000;73:43–50
  8. Virro MR, Larson-Cook KL, Evenson DP. Sperm chromatin structure assay (SCSA®) related to blastocyst rate, pregnancy rate and spontaneous abortion in IVF and ICSI cycles. Fertil Steril. 2004;8:1289–1295
  9. Evenson DP, Jost LK, Marshall D, Zinaman MJ, Clegg E, Purvis K, et al. Utility of the sperm chromatin structure assay (SCSA®) as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod. 1999;14:1039–1049
  10. Carrell DT, Liu L, Peterson CM, Jones KP, Hatasaka HH, Erickson L, et al. Sperm DNA fragmentation is increased in couples with unexplained recurrent pregnancy loss. Arch Androl. 2003;49:49–55
  11. Goldstein M, Gilbert BR, Dicker A, Dwosh J, Gnecco C. Microsurgical inguinal varicocelectomy with delivery of the testis: an artery and lymphatic sparing technique. J Urol. 1992;148:1808–1811
  12. Evenson DP, Larson KL, Jost LK. Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J Androl. 2002;23:25–43
  13. Ashkenazi J, Dicker D, Feldberg D, Shelef M, Goldman GA, Goldman J. The impact of spermatic vein ligation on the male factor in in vitro fertilization–embryo transfer and its relation to testosterone levels before and after operation. Fertil Steril. 1989;51:471–474
  14. Yamamoto M, Hibi H, Tsuji Y, Miyake K. The effect of varicocele ligation on oocyte fertilization and pregnancy after failure of fertilization in in vitro fertilization–embryo transfer. 1994;40:683–7.
  15. Schlegel PN. Is assisted reproduction the optimal treatment for varicocele-associated male infertility? A cost-effectiveness analysis. Urology. 1997;50:656
  16. Lin JC, Dhabuwala CB, Li H. The role of apoptosis in infertile men with varicoceles: Is the Fas system implicated?. J Urol. 2001;165(Suppl 4):1378A
  17. Henkel R, Hajimohammad M, Stalf T, Hoogendijk C, Mehnert C, Menkveld R, et al. Influence of deoxyribonucleic acid damage on fertilization and pregnancy. Fertil Steril. 2004;81:965–972
  18. Agarwal A, Said TM. Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach. BJU Int. 2005;95:503–507
  19. Hendin BN, Kolettis PN, Sharma RK, Thomas AJ, Agarwal A. Varicocele is associated with elevated spermatozoal reactive oxygen species production and diminished seminal plasma antioxidant capacity. J Urol. 1999;161:1831–1834
  20. Said TM, Paasch U, Glander HJ, Agarwal A. Role of caspases in male infertility. Hum Reprod Update. 2004;10:39–51
  21. Evenson DP, Wixon R. Meta-analysis of sperm DNA fragmentation using the Sperm Chromatin Structure Assay. Reprod BioMed Online. 2006;12:466–472

PII: S0015-0282(07)00831-X

doi: 10.1016/j.fertnstert.2006.09.019

Fertility and Sterility
Volume 90, Issue 5 , Pages 1800-1804 , November 2008

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