Fertility and Sterility
Volume 90, Issue 2 , Pages 328-334 , August 2008

Sperm DNA fragmentation in infertile men with genitourinary infection by Chlamydia trachomatis and Mycoplasma

  • Guadalupe Gallegos, M.D.

      Affiliations

    • Facultad de Medicina, Universidad Autónoma de Nuevo León, Nuevo León, México
    • ,
  • Benito Ramos, M.D.

      Affiliations

    • Facultad de Medicina, Universidad Autónoma de Nuevo León, Nuevo León, México
    • ,
  • Rebeca Santiso, Ph.D.

      Affiliations

    • Sección de Genética y Unidad de Investigación, Complejo Hospitalario Universitario Juan Canalejo, A Coruña, Spain
    • Laboratorio de Genética Molecular, Centro Oncológico de Galicia, A Coruña, Spain
    • ,
  • Vicente Goyanes, M.D., Ph.D.

      Affiliations

    • Sección de Genética y Unidad de Investigación, Complejo Hospitalario Universitario Juan Canalejo, A Coruña, Spain
    • ,
  • Jaime Gosálvez, Ph.D.

      Affiliations

    • Unidad de Genética, Facultad de Biología, Universidad Autónoma de Madrid, Spain
    • ,
  • José Luis Fernández, M.D., Ph.D.

      Affiliations

    • Sección de Genética y Unidad de Investigación, Complejo Hospitalario Universitario Juan Canalejo, A Coruña, Spain
    • Laboratorio de Genética Molecular, Centro Oncológico de Galicia, A Coruña, Spain
    • Corresponding Author InformationReprint requests: Dr. José Luis Fernández, Sección de Genética y Unidad de Investigación, Complejo Hospitalario Universitario Juan Canalejo, As Xubias, 84, 15006-A, Coruña, Spain (FAX: 34-981-287122).

Received 6 February 2007 ,Revised 12 June 2007

References 

  1. Paavonen J, Eggert-Kruse W. Chlamydia trachomatis: impact on human reproduction. Hum Reprod Update. 1999;5:433–447
  2. World Health Organization. In: Global prevalence and incidence of selected curable sexually transmited infections: overview and estimates. Geneva: World Health Organization; 2001;p. 1–43
  3. González GF, Muñoz G, Sánchez R, Henkel R, Gallegos-Avila G, Diaz-Gutierrez O, et al. Update on the impact of Chlamydia trachomatis infection on male infertility. Andrologia. 2004;36:1–23
  4. Veznik Z, Pospisil L, Svecova D, Zajicova A, Unzeitig V. Chlamydiae in the ejaculate: their influence in the quality and morphology of the sperm. Acta Obstet Gynecol Scand. 2004;83:656–660
  5. Hosseinzadeh S, Eley A, Pacey AA. Semen quality of men with asymptomatic clamydial infection. J Androl. 2004;25:104–109
  6. Jungwirtz A, Straberger B, Esterbauer K, Fink K, Schmeller N. Acrosome reaction in chlamydia-positive and negative patients. Andrologia. 2003;35:314–316
  7. Vigil P, Morales P, Tapia A, Riquelme R, Salgado AM. Chlamydia trachomatis infection in male partners of infertile couples: incidence and sperm function. Andrologia. 2002;34:155–161
  8. Idahl A, Boman J, Kumlin U, Olofsson JI. Demonstration of Chlamydia trachomatis IgG antibodies in the male partner of the infertile couple is correlated with a reduced likelihood of achieving a pregnancy. Hum Reprod. 2004;19:1121–1126
  9. Kilic D, Basar MM, Kaygusuz S, Yilmar E, Basar H, Batislam E. Prevalence and treatment of Chlamydia trachomatis, Ureaplasma urealyticum, and Mycoplasma hominis in patients with non-gonococcal urethritis. Jpn J Infect Dis. 2004;57:17–20
  10. Gnarpe H, Friberg J. Mycoplasma and human reproductive failure. I. The occurrence of different Mycoplasma in couples with reproductive failure. Am J Obstet Gynecol. 1972;114:727–731
  11. Gnarpe H, Friberg J. T-mycoplasmas on spermatozoa and infertility. Nature. 1973;245:97–98
  12. Fowlkes DM, MacLeod J, O'Leary WM. T-Mycoplasmas and human infertility: correlation of infection with alterations in seminal parameters. Fertil Steril. 1975;26:1212–1218
  13. De Louvois J, Beader M, Harrison RF, Hurley R, Stanley VC. Frequency of Mycoplasma in fertile and infertile couples. Lancet. 1974;1:1073–1075
  14. Andrade-Rocha FT. Ureaplasma urealyticum and Mycoplasma hominis in men attending for routine semen analysis. Prevalence, incidence by age and clinical settings, influence on sperm characteristics, relationship with the leukocyte count and clinical value. Urol Int. 2003;71:377–381
  15. Hosseinzadeh S, Brewis IA, Eley A, Pacey AA. Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death. Hum Reprod. 2001;16:293–299
  16. Nuñez-Calonge R, Caballero P, Redondo C, Baquero F, Martínez-Ferrer M, Meseguer MA. Ureaplasma urealyticum reduces motility and induces membrane alterations in human spermatozoa. Hum Reprod. 1998;13:2756–2761
  17. Eley A, Hosseinzadeh S, Hakimi H, Geary I, Pacey AA. Apoptosis of ejaculated human sperm is induced by co-incubation with Chlamydia trachomatis lipopolysaccharide. Hum Reprod. 2005;20:2601–2607
  18. Satta A, Stivala A, Garozzo A, Morello A, Perdichizzi A, Vicari E, et al. Experimental Chlamydia trachomatis infection causes apoptosis in human sperm. Hum Reprod. 2006;21:134–137
  19. World Health Organization. WHO laboratory manual for the examination of human semen and semen-cervical mucus interaction. Geneva: World Health Organization; 1999;
  20. Fernández JL, Muriel L, Goyanes V, Segrelles E, Gosálvez J, Enciso M, et al. Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion (SCD) test. Fertil Steril. 2005;84:833–842
  21. Hosseinzadeh S, Brewis IA, Pacey AA, Moore HDM, Eley A. Co-incubation of human spermatozoa with Chlamydia trachomatis in vitro causes increased tyrosine phosphorylation of sperm proteins. Infect Immun. 2000;68:4872–4876
  22. Hosseinzadeh S, Pacey AA, Eley A. Chlamydia trachomatis induced death in human spermatozoa is caused primarily by lipopolysaccharide. J Med Microbiol. 2003;52:193–200
  23. De Vries KJ, Wiedmer T, Sims PJ, Gadella BM. Caspase-independent exposure of aminophospholipids and tyrosine phosphorylation in bicarbonate responsive human sperm cells. Biol Reprod. 2003;68:2122–2134
  24. Sakkas D, Moffatt O, Manicardi GC, Mariethoz E, Tarozzi N, Bizzaro D. Nature of DNA damage in ejaculated human spermatozoa and the possible involvement of apoptosis. Biol Reprod. 2002;66:1061–1067
  25. Sakkas D, Manicardi GC, Bizzaro D. Sperm nuclear damage in the human. In:  Robaire B,  Hales BF editor. Advances in male mediated developmental toxicity. New York: Kluwer Academic/Plenum Publishers; 2003;p. 73–84
  26. Enciso M, Muriel L, Fernández JL, Goyanes V, Segrelles E, Marcos M, et al. Infertile men with varicocele show a high relative proportion of sperm cells with intense nuclear damage level, evidenced by the Sperm Chromatin Dispersion (SCD) test. J Androl. 2006;27:106–111
  27. Aitken RJ, Gordon E, Harkiss D, Twigg JP, Milne P, Jennings Z, et al. Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa. Biol Reprod. 1998;59:1037–1046
  28. Moustafa MH, Sharma RK, Thornton J, Mascha E, Abdel-Hafez MA, Thomas AJ, et al. Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Hum Reprod. 2004;19:129–138
  29. Henkel R, Kierspel E, Stalf T, Mehnert C, Menkveld R, Tinneberg HR, et al. Effect of reactive oxygen species produced by spermatozoa and leukocytes on sperm functions in non-leukocytospermic patients. Fertil Steril. 2005;83:635–642
  30. Eley A, Pacey AA, Galdiero M, Galdiero M, Galdiero F. Can Chlamydia trachomatis directly damage your sperm?. Lancet. 2005;5:53–57
  31. Potts JM, Sharma R, Pasqualotto F, Nelson D, Hall G, Agarwal A. Association of ureaplasma urealyticum with abnormal reactive oxygen species levels and absence of leukocytospermia. J Urol. 2000;163:1775–1778
  32. Crane D, Carlson J, Fischer E, Bavoil P, Hsia R, Tan C, et al. Chlamydia trachomatis polymorphic membrane protein D is a species-common pan-neutralizing antigen. PNAS. 2006;103:1894–1899

 The work has been possible through a grant from the Xunta de Galicia (PGIDIT 04BTF916023PR).

PII: S0015-0282(07)01378-7

doi: 10.1016/j.fertnstert.2007.06.035

Fertility and Sterility
Volume 90, Issue 2 , Pages 328-334 , August 2008

Article Tools