Fertility and Sterility
Volume 89, Issue 6 , Pages 1723-1727 , June 2008

Effect of cryopreservation on sperm DNA integrity in patients with teratospermia

  • Guruprasad Kalthur, Ph.D.

      Affiliations

    • Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India
    • ,
  • Satish Kumar Adiga, Ph.D.

      Affiliations

    • Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India
    • Corresponding Author InformationReprint requests: Satish Kumar Adiga, Ph.D., Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal-576 104, India (FAX: 91-820-25-71934).
    • ,
  • Dinesh Upadhya, M.Sc.

      Affiliations

    • Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India
    • ,
  • Satish Rao, Ph.D.

      Affiliations

    • Department of Radiobiology, Kasturba Medical College, Manipal University, Manipal, India
    • ,
  • Pratap Kumar, M.D.

      Affiliations

    • Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India

Received 11 February 2007 ,Revised 22 June 2007 ,Accepted 22 June 2007.

References 

  1. Critser JK, Arneson BW, Aaker DV, Huse-Benda AR, Ball GD. Cryopreservation of human spermatozoa. II. Post-thaw chronology of motility and of zona-free hamster ova penetration. Fertil Steril. 1987;47:980–984
  2. Yoshida H, Hoshiai H, Fukaya T, Yajima A. Fertilization of fresh and frozen spermatozoa. Assist Reprod Technol Androl. 1990;1:164–172
  3. Check ML, Check JH, Long R. Detrimental effects of cryopreservation on the structural and functional integrity of the sperm membrane. Arch Androl. 1991;27:155–160
  4. Cross NL, Hanks SE. Effects of cryopreservation on human sperm acrosomes. Hum Reprod. 1991;6:1279–1283
  5. Said TM, Aziz N, Sharma RK, Lewis-Jones I, Thomas AJ, Agarwal A. Novel association between sperm deformity index and oxidative stress–induced DNA damage in infertile male patients. Asian J Androl. 2005;7:121–126
  6. Watson PF. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod Fertil Dev. 1995;7:871–891
  7. Spano M, Cordelli E, Leter G, Lombardo F, Lenzi A, Gandini L. Nuclear chromatin variations in human spermatozoa undergoing swim-up and cryopreservation evaluated by the flow cytometric sperm chromatin structure assay. Mol Hum Reprod. 1999;5:29–37
  8. Gandini L, Lombardo F, Lenzi A, Spano M, Dondero F. Cryopreservation and sperm DNA integrity. Cell Tissue Bank. 2006;7:91–98
  9. Huret JL. Effect of cryopreservation on the nuclear chromatin decondensation ability of human spermatozoa. Arch Androl. 1984;12:33–38
  10. Watson PF, Morris GJ. Cold shock injury in animal cells. Symp Soc Exp Biol. 1987;41:311–340
  11. Darin-Bennett A, White IG. Influence of the cholesterol content of mammalian spermatozoa on susceptibility to cold-shock. Cryobiology. 1977;14:466–470
  12. Ballachey BE, Evenson DP, Saacke RG. The sperm chromatin structure assay. Relationship with alternate tests of semen quality and heterospermic performance of bulls. J Androl. 1988;9:109–115
  13. Evenson DP, Jost LK, Marshall D, Zinaman MJ, Clegg E, Purvis K, et al. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod. 1999;14:1039–1049
  14. World Health Organization . WHO laboratory manual. 4th ed.. Cambridge, UK: Cambridge University Press; 1999;
  15. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988;175:184–191
  16. Royere D, Hamamah S, Nicolle JC, Barthelemy C, Lansac J. Freezing and thawing alter chromatin stability of ejaculated human spermatozoa: fluorescence acridine orange staining and Fuelgen-DNA cytophotometric studies. Gamete Res. 1988;21:51–57
  17. Mortimer D, ed. Biochemistry of spermatozoa and seminal plasma. In: Practical laboratory andrology. New York: Oxford University Press, 1994:91–92.
  18. Wooley DM, Richardson DW. Ultrastructural injury to human spermatozoa after freezing and thawing. J Reprod Fertil. 1978;53:389–394
  19. Buffone MG, Doncel GF, Marín Briggiler CI, Vazquez-Levin MH, Calamera JC. Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation. Hum Reprod. 2004;19:139–146
  20. Long JA, Wildt DE, Wolfe BA, Critser JK, DeRossi RV, Howard J. Sperm capacitation and the acrosome reaction are compromised in teratospermic domestic cats. Biol Reprod. 1996;54:638–646
  21. Pukazhenthi BS, Wildt DE, Howard JG. The phenomenon and significance of teratospermia in felids. J Reprod Fertil. 2001;57(Suppl):423–433
  22. Irvine DS, Twigg JP, Gordon EL, Fulton N, Milne PA, Aitken RJ. DNA integrity in human spermatozoa: relationships with semen quality. J Androl. 2000;21:33–44
  23. McCosker PJ. Abnormal spermatozoan chromatin in infertile bulls. J Reprod Fertil. 1969;18:363–365
  24. Gandini L, Lombardo F, Paoli D, Caponecchia L, Familiari G, Verlengia C, et al. Study of apoptotic DNA in human spermatozoa. Hum Reprod. 2000;14:830–839
  25. Donnelly ET, Steele EK, McClure N, Lewis SE. Assessment of DNA integrity and morphology of ejaculated spermatozoa from fertile and infertile men before and after cryopreservation. Hum Reprod. 2001;16:1191–1199
  26. Gravance CG, Vishwanath R, Pitt C, Garner DL, Casey PJ. Effects of cryopreservation on bull sperm head morphometry. J Androl. 1998;19:704–709
  27. Paasch U, Sharma RK, Gupta AK, Grunewald S, Mascha EJ, Thomas AJ, et al. Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Biol Reprod. 2004;71:1828–1837
  28. Baumber J, Ball BA, Linfor JJ, Meyers SA. Reactive oxygen species and cryopreservation promote DNA fragmentation in equine spermatozoa. J Androl. 2003;24:621–628
  29. Pasqualotto F, Sharma R, Kobayashi H, Nelson D, Thomas AJ, Agarwal A. Oxidative stress in normospermic men undergoing infertility evaluation. J Androl. 2001;22:316–322
  30. Van Loon AA, Den Boer PJ, Van der Schans GP, Mackenbach P, Grootegoed JA, Baan RA, et al. Immunochemical detection of DNA damage induction and repair at different cellular stages of spermatogenesis of the hamster after in vitro or in vivo exposure to ionizing radiation. Exp Cell Res. 1991;193:303–309
  31. Van Loon AA, Sonneveld E, Hoogerbrugge J, van der Schans GP, Grootegoed JA, Lohman PH, et al. Induction and repair of DNA single-strand breaks and DNA base damage at different cellular stages of spermatogenesis of the hamster upon in vitro exposure to ionizing radiation. Mutat Res. 1993;294:139–148
  32. Drost JB, Lee WR. Biological basis of germline mutation: comparisons of spontaneous germline mutation rates among drosophila, mouse, and human. Environ Mol Mutagen. 1995;25(Suppl 26):48–64
  33. Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z. Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells: analogy to apoptosis of somatic cells. Exp Cell Res. 1993;207:202–205

PII: S0015-0282(07)01441-0

doi: 10.1016/j.fertnstert.2007.06.087

Fertility and Sterility
Volume 89, Issue 6 , Pages 1723-1727 , June 2008

Article Tools

* Image Usage & Resolution