The p21 codon 31∗C- and DRD2 codon 313∗T-related genotypes/alleles, but not XRCC1 codon 399, hOGG1 codon 326, and DRD1-48 polymorphisms, are correlated with the presence of leiomyoma

Hsieh, Yao-Yuan; Chang, Chi-Chen; Bau, Da-Tian; Tsai, Fuu-Jen; Tsai, Chang-Hai; Chen, Chih-Ping

doi:10.1016/j.fertnstert.2007.07.1328

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Volume 91, Issue 3 , Pages 869-877, March 2009

The p21 codon 31^∗C- and DRD2 codon 313^∗T-related genotypes/alleles, but not XRCC1 codon 399, hOGG1 codon 326, and DRD1-48 polymorphisms, are correlated with the presence of leiomyoma

Yao-Yuan Hsieh, M.D., Ph.D.
- Department of Obstetrics and Gynecology, China Medical University Hospital, Taipei, Taiwan, People's Republic of China
Chi-Chen Chang, M.D.
- Department of Obstetrics and Gynecology, China Medical University Hospital, Taipei, Taiwan, People's Republic of China
Da-Tian Bau, Ph.D.
- Department of Medical Research, China Medical University Hospital, Taipei, Taiwan, People's Republic of China
- Graduate Institute of Chinese Medical Science, China Medical University, Taipei, Taiwan, People's Republic of China
Fuu-Jen Tsai, M.D., Ph.D.
- Department of Medical Research, China Medical University Hospital, Taipei, Taiwan, People's Republic of China
- Graduate Institute of Chinese Medical Science, China Medical University, Taipei, Taiwan, People's Republic of China
- Reprints requests: Fuu-Jen Tsai, M.D., Ph.D., Department of Pediatrics and Medical Genetics, China Medical University Hospital, No. 2 Yuh-Der Road, Taichung, Taiwan, People's Republic of China (FAX: 886-4-22033295).
Chang-Hai Tsai, M.D., Ph.D.
- Department of Medical Research, China Medical University Hospital, Taipei, Taiwan, People's Republic of China
- Taichung Health Care and Management University, Taichung, Taiwan, China
Chih-Ping Chen, M.D.
- Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan, People's Republic of China

Received 13 May 2007; received in revised form 12 July 2007; accepted 12 July 2007. published online 27 January 2009.

Objective

To investigate whether the gene polymorphisms for p21, X-ray repair cross-complementing group 1 (XRCC1), human 8-oxoguanine glycosylase 1 (hOGG1), and dopamine D1 and D2 receptors (DRD1, -2) are associated with leiomyoma susceptibility.

Design

Prospective study.

Setting

Departments of gynecology and genetics in a medical center.

Patient(s)

Women were divided into two groups: leiomyoma (n = 120) and nonleiomyoma (n = 112).

Intervention(s)

The p21 codon 31, XRCC1 codon 399, hOGG1 codon 326, DRD1-48, and DRD2 codon 313 polymorphisms were genotyped by polymerase chain reaction with restriction enzyme digestions (Blp I, MspI, Fnu4HI, Dde I, and NcoI, respectively).

Main Outcome Measure(s)

Genotypes and allelic frequencies.

Result(s)

The p21 codon 31^∗C- and DRD2 codon 313^∗T-related genotypes/alleles were associated with the presence of leiomyomas. The proportions of p21^∗CC/CA/AA and DRD2^∗CC/CT/TT in both groups were 27.5/68.3/4.2% and 12.5/51.7/35.8% (leiomyoma); and 14.3/51.8/33.9% and 33.9/40.2/25.9% (nonleiomyoma). XRCC1, hOGG1, and DRD1 were not correlated with the presence of leiomyomas. XRCC1^∗GG/GA/AA, hOGG1^∗TT/TA/AA, and DRD1^∗GG/GA/AA were 54.2/37.5/8.3%, 36.7/44.2/19.1%, and 3.3/25.8/70.8% (leiomyoma); and 48.2/47.3/4.5%, 43.6/41/15.4%, and 3.6/25/71.4% (nonleiomyoma).

Conclusion(s)

The p21 codon 31^∗C- and DRD2 codon 313^∗T-related genotypes/alleles were associated with the presence of leiomyoma. XRCC1, hOGG1, and DRD1 were not correlated with leiomyoma development.

Key Words: DRD, hOGG1, leiomyoma, p21, polymorphism, XRCC1