Fertility and Sterility
Volume 90, Issue 4 , Pages 972-987, October 2008

Gene expression profiles and functional characterization of human immortalized endometriotic epithelial and stromal cells

  • Sakhila K. Banu, Ph.D.

      Affiliations

    • Reproductive Endocrinology and Cell Signaling Laboratory, Department of Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas
    • ,
  • JeHoon Lee, M.S.

      Affiliations

    • Reproductive Endocrinology and Cell Signaling Laboratory, Department of Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas
    • ,
  • Anna Starzinski-Powitz, Ph.D.

      Affiliations

    • Institut der Anthropologie und Humangenetik fuer Biologen, Johann-Wolfgang-Goethe-Universitaet, Frankfurt, Germany
    • ,
  • Joe A. Arosh, Ph.D.

      Affiliations

    • Reproductive Endocrinology and Cell Signaling Laboratory, Department of Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas
    • Corresponding Author InformationReprint requests: Joe A. Arosh, Ph.D., Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Mail Stop: TAMU 4458, Texas A&M University, College Station, TX 77843 (FAX: 979-847-8981).

Received 30 April 2007; received in revised form 29 June 2007; accepted 23 July 2007. published online 14 November 2007.

Objective

To determine whether immortalized endometriotic cells can be used as potential models to unravel pathophysiology/pathogenesis of endometriosis in humans.

Design

In vitro study. Human immortalized endometriotic epithelial and stromal cells.

Setting

College of Veterinary Medicine and Biomedical Sciences, Texas A&M University.

Patient(s)

Not applicable.

Intervention(s)

None.

Main Outcome Measure(s)

Gene expression pattern and prostaglandin E2 production and activity of matrix metalloproteinase 2 and matrix metalloproteinase 9.

Result(s)

Several genes associated with estrogen and progesterone biosynthesis and signaling, cell cycle regulation, extracellular matrix degradation, angiogenesis, cell growth and survival, cytokine production, and prostaglandin E2 biosynthesis, transport, and signaling were expressed in human immortalized endometriotic cells. These endometriotic epithelial and stromal cells exhibited migrating and invading potential, produced a high quantity of prostaglandin E2, and showed higher matrix metalloproteinase 2 and matrix metalloproteinase 9 activity compared to normal endometrial, epithelial, and stromal cells.

Conclusion(s)

Human immortalized endometriotic epithelial and stromal cells could be used as ideal models to study the molecular and cellular aspects of endometriosis in humans.

Key Words: Endometriosis, immortalized, endometriotic cells, COX-2, PGE2, MPP2 and MMP9, endometriotic cell migration and invasion

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 Supported by Texas A&M University, College Station, Texas.

PII: S0015-0282(07)03011-7

doi:10.1016/j.fertnstert.2007.07.1358

Fertility and Sterility
Volume 90, Issue 4 , Pages 972-987, October 2008

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