Fertility and Sterility
Volume 90, Issue 5 , Pages 1649-1655 , November 2008

Resumption of mitosis in frozen–thawed embryos is not related to the chromosomal constitution

  • Inge E. Agerholm, M.Sc.

      Affiliations

    • The Fertility Clinic, Braedstrup Hospital, Braedstrup, Denmark
    • Corresponding Author InformationReprint requests: Inge E. Agerholm, M.Sc., Fertility Clinic, Braedstrup Hospital, Women's Clinic, Sygehusvej 20, DK 8740 Braedstrup, Denmark.
  • ,
  • Steen Kølvraa, M.D., D.Sci.

      Affiliations

    • Department of Clinical Genetics, Vejle Hospital, Vejle, Denmark
  • ,
  • Dorthe G. Crüger, M.D., Ph.D.

      Affiliations

    • Department of Clinical Genetics, Vejle Hospital, Vejle, Denmark
  • ,
  • Charlotte Berg

      Affiliations

    • Department of Clinical Genetics, Vejle Hospital, Vejle, Denmark
  • ,
  • Gert Bruun-Petersen, M.D., D.Sci.

      Affiliations

    • Department of Clinical Genetics, Vejle Hospital, Vejle, Denmark
  • ,
  • Søren Ziebe, M.Sc.

      Affiliations

    • The Fertility Clinic, Rigshospitalet, Copenhagen, Denmark

Received 2 April 2007 ,Revised 2 August 2007 ,Accepted 2 August 2007.

References 

  1. Van der Elst J, Van den Abbeel E, Vitrier S, Camus M, Devroey P, Van Steirteghem AC. Selective transfer of cryopreserved human embryos with further cleavage after thawing increases delivery and implantation rates. Hum Reprod. 1997;12:1513–1521
  2. Ziebe S, Bech B, Petersen K, Mikkelsen AL, Gabrielsen A, Andersen AN. Resumption of mitosis during post-thaw culture: a key parameter in selecting the right embryos for transfer. Hum Reprod. 1998;13:178–181
  3. Gabrielsen A, Fedder J, Agerholm I. Parameters predicting the implantation rate of thawed IVF/ICSI embryos: a retrospective study. Reprod Biomed Online. 2004;12:70–76
  4. Guerif F, Bidault R, Cadoret V, Couet ML, Lansac J, Royere D. Parameters guiding selection of best embryos for transfer after cryopreservation: a reappraisal. Hum Reprod. 2002;17:1321–1326
  5. Van den Abbeel E, Camus M, van Waesberghe L, Devroey P, Van Steirteghem AC. Viability of partially damaged human embryos after cryopreservation. Hum Reprod. 1997;12:2006–2010
  6. Edgar DH, Bourne H, Speirs AL, McBain JC. A quantitative analysis of the impact of cryopreservation on the implantation potential of human early cleavage stage embryos. Hum Reprod. 2000;15:175–179
  7. El-Toukhy T, Khalaf Y, Al-Darazi K, O'Mahone F, Taylor A, Braude P. Effect of blastomere loss on the outcome of frozen embryo replacement cycles. Fertil Steril. 2003;79:1106–1111
  8. Archer J, Gook DA, Edgar DH. Blastocyst formation and cell number in human frozen–thawed embryos following extended culture. Hum Reprod. 2003;18:1669–1673
  9. Salumets A, Tuuri T, Mäkinen S, Vilska S, Husu L, Tainio R, et al. Effect of developmental stage of embryo at freezing on pregnancy outcome of frozen–thawed embryo transfer. Hum Reprod. 2003;18:1890–1895
  10. Ziebe S, Petersen K, Lindenberg S, Andersen AG, Gabrielsen A, Andersen AN. Embryo morphology and cleavage stage: how to select the best embryos for transfer after in-vitro fertilization. Hum Reprod. 1997;12:1545–1549
  11. Iwarsson E, Lundqvist M, Inzunza J, Ährlund-Richter L, Sjöblom P, Lundkvist Ô, et al. A high degree of aneuploidy in frozen-thawed human preimplantation embryos. Hum Genet. 1999;104:376–382
  12. Laverge H, Van der Elst J, De Sutter P, Verschraegen-Spae MR, De Paepe A, Dhont M. Fluorescent in-situ hybridization in human embryos showing cleavage arrest after freezing and thawing. Hum Reprod. 1998;12:425–429
  13. Baart EB, Van Opstal D, Los FJ, Fauser BCJM, Martini E. Fluorescence in situ hybridization analysis of two blastomeres from day 3 frozen–thawed embryos followed by analysis of the remaining embryo on day 5. Hum Reprod. 2004;19:685–693
  14. Testart J, Lassalle B, Belaisch-Allart J, Hazout A, Farman R, Rainhorn JD, et al. High pregnancy rate after early human embryo freezing. Fertil Steril. 1986;2:268–272
  15. Coonen E, Dumoulin JC, Ramaekers FC, Hopman AH. Optimal preparation of preimplantation embryo interphase nuclei for analysis by fluorescence in-situ hybridization. Hum Reprod. 1994;9:533–537
  16. Agerholm I, Ziebe B, Williams B, Berg C, Crüger DG, Bruun-Petersen G, et al. Sequential FISH analysis using competitive displacement of labelled peptide nucleic acid probes for eight chromosomes in human blastomeres. Hum Reprod. 2005;20:1072–1077
  17. Ziebe S, Lundin K, Loft A, Bergh C, Nyboe Andersen AN, Selleskog U, et al. FISH analysis for chromosomes 13, 16, 18, 21, 22, X and Y in all blastomeres of IVF pre-embryos from 144 randomly selected donated human oocytes and impact on pre-embryo morphology. Hum Reprod. 2003;18:2575–2581
  18. Munne S, Alikani M, Tomkin G, Grifo J, Cohen J. Embryo morphology, developmental rates, and maternal age are correlated with chromosome abnormalities. Fertil Steril. 1995;64:382–391
  19. Valojerdi MR, Salehnia M. Developmental potential and ultrastructural injuries of metaphase II (MII) mouse oocytes after slow freezing or vitrification. J Assist Reprod Genet. 2005;22:119–127
  20. Huang JY, Chen HY, Tan SL, Chian RC. Effect of choline-supplemented sodium-depleted slow freezing versus vitrification on mouse oocyte meiotic spindles and chromosome abnormalities. Fertil Steril. June 1: [Epub ahead of print].
  21. Salumets A, Horelli-Kuitunen N, Suikkari AM, Metspalu A, Tuuri T. Elevated incidence of chromosomally chaotic embryos among frozen-thawed preimplantation embryos. Eur J Obstet Gynecol. 2004;114:59–63
  22. Rienzi L, Nagy ZP, Ubaldi F, Iacobelli M, Anniballo R, Tesarik J, et al. Laser-assisted removal of necrotic blastomeres from cryopreserved embryos that were partially damaged. Fertil Steril. 2002;77:1196–1201
  23. Van Royen E, Mangelschots K, Vercruyssen M, De Neubourg D, Valkenburg M, Ryckaert G, et al. Multinucleation in cleavage stage embryos. Hum Reprod. 2003;18:1062–1069
  24. Meriano J, Clark C, Cadesky K, Laskin C. Binucleated and micronucleated blastomeres in embryos derived from human assisted reproduction cycles. Reprod Biomed Online. 2004;9:511–520
  25. Hardy K, Winston RM, Handyside AH. Binucleate blastomeres in preimplantation human embryos in vitro: failure of cytokinesis during early cleavage. J Reprod Fertil. 1993;98:549–558
  26. Pickering SJ, Taylor A, Johnsom MH, Braude PR. An analysis of multinuclear blastomeres and their derivative daughter blastomeres. Hum Reprod. 1995;10:1912–1922
  27. Hnida C, Engenheiro E, Ziebe S. Computer-controlled, multilevel, morphometric analysis of blastomere size as biomarker of fragmentation and multinuclearity in human embryos. Hum Reprod. 2004;19:288–293

 Supported by the Danish Medical Council (Vejle, Denmark) and the Vejle Council (Vejle, Denmark).

PII: S0015-0282(07)03158-5

doi: 10.1016/j.fertnstert.2007.08.015

Fertility and Sterility
Volume 90, Issue 5 , Pages 1649-1655 , November 2008