Fertility and Sterility
Volume 90, Issue 4, Supplement , Pages 1503-1510 , October 2008

Comparison of the efficacy of two commercially available media for culturing one-cell embryos in the in vitro fertilization mouse model

  • Paulo Marcelo Perin, M.D.

      Affiliations

    • Department of Pathology, University of São Paulo School of Medicine, São Paulo, Brazil
    • Division of Reproductive Medicine, CEERH, Specialized Center for Human Reproduction, São Paulo, Brazil
    • Corresponding Author InformationReprint requests: Paulo Marcelo Perin, M.D., Division of Reproductive Medicine, CEERH–Specialized Center for Human Reproduction, R. Pará, n. 50, 12th floor, São Paulo 01243-0020, Brazil (FAX: 55-11-3258-0008).
    • ,
  • Mariangela Maluf, M.D.

      Affiliations

    • Department of Pathology, University of São Paulo School of Medicine, São Paulo, Brazil
    • Division of Reproductive Medicine, CEERH, Specialized Center for Human Reproduction, São Paulo, Brazil
    • ,
  • Daniela Aparecida Nicolosi Foltran Januário, B.Sc.

      Affiliations

    • Department of Experimental Physiopathology, University of São Paulo School of Medicine, São Paulo, Brazil
    • Division of Reproductive Medicine, CEERH, Specialized Center for Human Reproduction, São Paulo, Brazil
    • ,
  • Paulo Hilário Nascimento Saldiva, M.D., Ph.D.

      Affiliations

    • Department of Pathology, University of São Paulo School of Medicine, São Paulo, Brazil

Received 23 March 2007 ,Revised 29 September 2007 ,Accepted 29 September 2007.

References 

  1. Harris C, Hansen J. In vitro methods for the study of mechanisms of developmental toxicology. In:  Hood R editors. Developmental and reproductive toxicology—a practical approach. Boca Raton, FL: CRC Press; 2006;p. 647–695
  2. Suzuki O, Asano T, Yamamoto Y, Takano K, Koura MK. Development in vitro of preimplantation embryos from 55 mouse strains. Reprod Fertil. 1996;8:975–980
  3. Menezo Y. Mouse and bovine models for human IVF. Reprod BioMed Online. 2002;4:170–175
  4. Gardner D, Lane M. Embryo culture systems. In:  Trounson A,  Gardner D editor. Handbook of in vitro fertilization. Boca Raton, FL: CRC Press; 1999;p. 205–264
  5. Biggers J. Thoughts on embryo culture conditions. Reprod BioMed Online. 2002;4(Suppl 1):30–38
  6. Goddard M, Pratt H. Control of events during early cleavage of the mouse embryo: an analysis of the 2-cell block. J Embryol Exp Morphol. 1983;73:111–133
  7. Gardner D, Lane M. Alleviation of the 2-cell block and development to the blastocyst of CF1 mouse embryos: role of amino acids, EDTA and physical parameters. Hum Reprod. 1996;11:2703–2712
  8. Lawitts J, Biggers J. Joint effects of sodium chloride, glutamine, and glucose in mouse preimplantation embryo culture media. Mol Reprod Dev. 1992;31:189–194
  9. Ho Y, Wigglesworth K, Eppig J, Schultz R. Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression. Mol Reprod Dev. 1995;41:232–238
  10. Summers M, McGinnis L, Lawitts J, Raffin M, Biggers J. IVF of mouse ova in a simplex optimized medium supplemented with amino acids. Hum Reprod. 2000;15:1791–1801
  11. Barnes F, Crombie A, Gardner D, Kausche A, Lacham-Kaplan O, Suikkari A, et al. Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod. 1995;10:3243–3247
  12. Lane M, Gardner D. Ammonium induces aberrant blastocyst differentiation, metabolism, pH regulation, gene expression and subsequently alters fetal development in the mouse. Biol Reprod. 2003;69:1109–1117
  13. Biggers J, McGinnis L, Lawitts J. One-step versus two-step culture of mouse preimplantation embryos: is there a difference?. Hum Reprod. 2005;20:3376–3384
  14. Gardner D, Lane M. Amino acids and ammonium regulate mouse embryo development in culture. Biol Reprod. 1993;48:377–385
  15. Thouas G, Korfiatis N, French A, Jones G, Trounson A. Simplified technique for differential staining of inner cell mass and trophectoderm cells of mouse and bovine blastocysts. Reprod BioMed Online. 2001;3:25–29
  16. Erbach G, Lawitts J, Papaioannou V, Biggers J. Differential growth of the mouse preimplantation embryo in chemically defined media. Biol Reprod. 1994;50:1027–1033
  17. Summers M, Bhatnagar P, Lawitts J, Biggers J. Fertilization in vitro of mouse ova from inbred and outbred strains: complete preimplantation embryo development in glucose-supplemented KSOM. Biol Reprod. 1995;53:431–437
  18. Summers M, McGinnis L, Lawitts J, Biggers J. Mouse embryo development following IVF in media containing either L-glutamine or glycyl-L-glutamine. Hum Reprod. 2005;20:1364–1371
  19. Xu J, Chan S, Lee W, Lee K, Yeung W. Differential growth, cell proliferation, and apoptosis of mouse embryo in various culture media and in coculture. Mol Reprod Dev. 2004;68:72–80
  20. Karagenc L, Lane M, Gardner D. Granulocyte-macrophage colony-stimulating factor stimulates mouse blastocyst inner cell mass development only when media lack human serum albumin. Reprod BioMed Online. 2005;10:511–518
  21. Iwasaki S, Yoshiba N, Ushijima H, Watanabe S, Nakahara T. Morphology and proportion of inner cell mass of bovine blastocysts fertilized in vitro and in vivo. J Reprod Fertil. 1990;90:279–284
  22. Leppens G, Gardner D, Sakkas D. Co-culture of 1-cell outbred mouse embryos on bovine kidney epithelial cells: effect on development, glycolytic activity, inner cell mass:trophectoderm ratios and viability. Hum Reprod. 1996;11:598–603
  23. Lane M, Gardner D. Differential regulation of mouse embryo development and viability by amino acids. J Reprod Fertil. 1997;109:153–164
  24. Fouladi-Nashta A, Alberio R, Kafi M, Nicholas B, Campbell K, Webb R. Differential staining combined with TUNEL labelling to detect apoptosis in preimplantation bovine embryos. Reprod BioMed Online. 2005;10:497–502
  25. Summers M, Biggers J. Chemically defined media and the culture of mammalian preimplantation embryos: historical perspective and current issues. Hum Reprod Update. 2003;9:557–582
  26. Pool T. Recent advances in the production of viable human embryos in vitro. Reprod BioMed Online. 2002;4:294–302
  27. Gardner D, Lane M. Towards a single embryo transfer. Reprod BioMed Online. 2003;6:470–481
  28. Wang Y, Puscheck E, Lewis J, Trostinskaia A, Wang F, Rappolee D. Increases in phosphorylation of SAPK/JNK and p38MAPK correlate negatively with mouse embryo development after culture in different media. Fertil Steril. 2005;83(Suppl 1):1144–1154
  29. Fong B, Watson P, Watson A. Mouse preimplantation embryo responses to culture medium osmolarity include increased expression of CCM2 and p38 MAPK activation. BMC Dev Biol. 2007;7:2–17
  30. Hadi T, Hammer M, Algire C, Richards T, Baltz J. Similar effects of osmolarity, glucose, and phosphate on cleavage past the 2-cell stage in mouse embryos from outbred and F1 hybrid females. Biol Reprod. 2005;72:179–187

PII: S0015-0282(07)03758-2

doi: 10.1016/j.fertnstert.2007.09.062

Fertility and Sterility
Volume 90, Issue 4, Supplement , Pages 1503-1510 , October 2008

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