Fertility and Sterility
Volume 90, Issue 1 , Pages 77-83 , July 2008

Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study

  • Richard Scott, M.D.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey; Department of Obstetrics, Gynecology, and Reproductive Sciences, UMDNJ–Robert Wood Johnson Medical School, New Brunswick, New Jersey
    • ,
  • Emre Seli, M.D.

      Affiliations

    • Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut
    • ,
  • Kathy Miller, B.S.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey; Department of Obstetrics, Gynecology, and Reproductive Sciences, UMDNJ–Robert Wood Johnson Medical School, New Brunswick, New Jersey
    • ,
  • Denny Sakkas, Ph.D.

      Affiliations

    • Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut
    • ,
  • Katherine Scott, B.S.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey; Department of Obstetrics, Gynecology, and Reproductive Sciences, UMDNJ–Robert Wood Johnson Medical School, New Brunswick, New Jersey
    • ,
  • David H. Burns, Ph.D.

      Affiliations

    • Department of Chemistry, McGill University, Montreal, Quebec, Canada
    • Corresponding Author InformationReprint requests: David H. Burns, Ph.D., Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal QC H3A 2K6, Canada (FAX: 514-398-3797).

Received 11 February 2007 ,Revised 20 November 2007 ,Accepted 20 November 2007.

References 

  1. SART . Assisted reproductive technology success rates. National summary and fertility clinic reports. Atlanta, GA: Centers for Disease Control; 2004;
  2. Scott LA, Smith S. The successful use of pronuclear embryo transfer the day following oocyte retrieval. Hum Reprod. 1998;13:1003–1013
  3. Tesarik J, Greco E. The probability of abnormal preimplantation development can be predicted by a single static observation on pronuclear state morphology. Hum Reprod. 1999;14:1318–1323
  4. Shoukir Y, Campana A, Farley T, Sakkas D. Early cleavage of in-vitro fertilized human embryos to the 2-cell stage: a novel indicator of embryo quality and viability. Hum Reprod. 1997;12:1531–1536
  5. Sakkas D, Percival G, D'Arcy Y, Sharif K, Afnan M. Assessment of early cleaving in vitro fertilized human embryos at the 2-cell stage before transfer improves embryo selection. Fertil Steril. 2001;76:1150–1156
  6. Van Royen E, Mangelschots K, De Neubourg D, Valkenburg M, Van de Meerssche M, Ryckaert G, et al. Characterization of a top quality embryo, a step towards single-embryo transfer. Hum Reprod. 1999;14:2345–2349
  7. Antczak M, Van Blerkom J. Temprol and spatial aspect of fragmentation in early human embryos: possible effects on developmental competence and association with the differential elimination of regulatory proteins from polarized domains. Human Reprod. 1999;14:429–447
  8. De Neubourg D, Gerris J, Mangelschots K, Van Royen E, Vercruyssen M, Elseviers M. Single top quality embryo transfer as a model for prediction of early pregnancy outcome. Hum Reprod. 2004;19:1476–1479
  9. Toner JP. Progress we can be proud of: U.S. trends in assisted reproduction over the first 20 years. Fertil Steril. 2002;78:943–950
  10. Gardner DK, Lane M, Stevens J, Schoolcraft WB. Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential. Fertil Steril. 2001;76:1175–1180
  11. Conaghan J, Hardy K, Handyside AH, Winston RM, Leese HJ. Selection criteria for human embryo transfer: a comparison of pyruvate uptake and morphology. J Assist Reprod Genet. 1993;10:21–30
  12. Houghton FD, Hawkhead JA, Humpherson PG, Hogg JE, Bolen AH, Rutherford AJ, et al. Non-invasive amino acid turnover predicts human embryo developmental capacity. Hum Reprod. 2002;17:999–1005
  13. Brison DR, Houghton FD, Falconer D, Roberts SA, Hawkhead J, Humpherson PG, et al. Identification of viable embryos in IVF by non-invasive measurement of amino acid trunover. Hum Reprod. 2004;19:2319–2324
  14. Seli E, Sakkas D, Scott R, Kwok JS, Rosendahl S, Burns DH. Non-invasive metabolomic profiling of human embryo culture media using Raman and near infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization. Fertil Steril. 2007;88:1350–1357
  15. Muasher SJ, Abdallah RT, Hubayter ZR. Optimal stimulation protocols for in vitro fertilization. Fertil Steril. 2006;86:267–273
  16. Burns DA, Ciurczak EK. Handbook of near-infrared analysis. New York: Marcel Dekker; 2001;
  17. Gributs CEW, Burns DH. In vivo near-infrared spectroscopy. In:  Chalmers JM,  Griffiths PR editor. Handbook of vibrational spectroscopy. Chichester: John Wiley; 2002;p. 3362–3375
  18. Siesler HW, Ozaki Y, Kawata S, Heise HM. Near-infrared spectroscopy. Weinheim: Wiley-VCH; 2002;
  19. Williams P. Vibrational near-infrared spectroscopy—application to grains and derived commodoties. In:  Chalmers JM,  Griffiths PR editor. Handbook of vibrational spectroscopy. Chichester: John Wiley; 2002;p. 3693–3719
  20. Alam MK. Noninvasive diagnoses by near-infrared spectroscopy. In:  Chalmers JM,  Griffiths PR editor. Handbook of vibrational spectroscopy. Chichester: John Wiley; 2002;p. 3266–3279
  21. Enejder AMJ, Koo TW, Oh J, Hunter M, Sasic S, Feld MS, et al. Blood analysis by Raman spectroscopy. Opt Lett. 2002;27:2004–2006

 One of the authors, D.B., has an equity interest in the company developing this technology for clinical application (Molecular Biometrics, LLC). Three authors, R.S., E.S., and D.S., serve on a scientific advisory board of Molecular Biometrics.

PII: S0015-0282(07)04123-4

doi: 10.1016/j.fertnstert.2007.11.058

Fertility and Sterility
Volume 90, Issue 1 , Pages 77-83 , July 2008

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