Fertility and Sterility
Volume 91, Issue 2 , Pages 627-631 , February 2009

Evaluation of a disposable plastic Neubauer counting chamber for semen analysis

  • Jackson Kirkman-Brown, Ph.D.

      Affiliations

    • Centre for Human Reproductive Science, The Assisted Conception Unit, Birmingham Women's Health Care Trust, and Division of Reproductive and Child Health, The Medical School, The University of Birmingham, Birmingham, West Midlands, United Kingdom
    • ,
  • Lars Björndahl, M.D., Ph.D.

      Affiliations

    • Centre for Human Reproductive Science, The Assisted Conception Unit, Birmingham Women's Health Care Trust, and Division of Reproductive and Child Health, The Medical School, The University of Birmingham, Birmingham, West Midlands, United Kingdom
    • Centre for Andrology and Sexual Medicine, Karolinska University Hospital, Huddinge, and Karolinska Institutet, Stockholm, Sweden
    • Corresponding Author InformationReprint requests: Lars Björndahl, M.D., Ph.D., Centre for Andrology and Sexual Medicine, Karolinska University Hospital, Huddinge M52, S-141 86 Huddinge, Sweden (FAX: 46-8-31-36-91).

Received 13 September 2007 ,Revised 22 November 2007 ,Accepted 26 November 2007.

References 

  1. World Health Organization . WHO laboratory manual for the examination of human semen and sperm-cervical mucus interactions. 4th ed.. New York: Cambridge University Press; 1999;
  2. In:  Kvist U,  Björndahl L editor. Manual on basic semen analysis. Oxford: Oxford University Press; 2002;
  3. Mortimer D, Shu MA, Tan R, Mortimer ST. A technical note on diluting semen for the haemocytometric determination of sperm concentration. Hum Reprod. 1989;4:166–168
  4. Mortimer D, Shu MA, Tan R. Standardization and quality control of sperm concentration and sperm motility counts in semen analysis. Hum Reprod. 1986;1:299–303
  5. Mahmoud AM, Depoorter B, Piens N, Comhaire FH. The performance of 10 different methods for the estimation of sperm concentration. Fertil Steril. 1997;68:340–345
  6. Tomlinson M, Turner J, Powell G, Sakkas D. One-step disposable chambers for sperm concentration and motility assessment: how do they compare with the World Health Organization's recommended methods?. Hum Reprod. 2001;16:121–124
  7. Douglas-Hamilton DH, Smith NG, Kuster CE, Vermeiden JP, Althouse GC. Capillary-loaded particle fluid dynamics: effect on estimation of sperm concentration. J Androl. 2005;26:115–122
  8. Douglas-Hamilton DH, Smith NG, Kuster CE, Vermeiden JP, Althouse GC. Particle distribution in low-volume capillary-loaded chambers. J Androl. 2005;26:107–114
  9. Riddell D, Pacey A, Whittington K. Lack of compliance by UK andrology laboratories with World Health Organization recommendations for sperm morphology assessment. Hum Reprod. 2005;20:3441–3445

 Presented as a poster at the Annual Meeting of the Nordic Association for Andrology in Stockholm, Sweden, August 31–September 1, 2006.

PII: S0015-0282(07)04141-6

doi: 10.1016/j.fertnstert.2007.11.076

Fertility and Sterility
Volume 91, Issue 2 , Pages 627-631 , February 2009

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