Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human tubal fluid

Medeiros de Araujo, Carlos Henrique; Nogueira, Daniela; de Araujo, Maria Cristina Picinato Medeiros; de Paula Martins, Wellington; Ferriani, Rui Alberto; dos Reis, Rosana Maria

doi:10.1016/j.fertnstert.2007.11.082

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Volume 91, Issue 2 , Pages 509-513, February 2009

Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human tubal fluid

Carlos Henrique Medeiros de Araujo, Ph.D.
- Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil
- Reprint requests: Carlos Henrique Medeiros de Araujo, Ph.D., Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, Avenida dos Bandeirantes 3900, Ribeirão Preto 14049-900, SP, Brazil (FAX: 55-16-36332810).
Daniela Nogueira, Ph.D.
- Laboratory of Reproductive Biology-IFREARES, Clinique Saint-Jean Languedoc, Toulouse, France
Maria Cristina Picinato Medeiros de Araujo, B.Sc.
- Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil
Wellington de Paula Martins, Ph.D.
- Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil
Rui Alberto Ferriani, Ph.D.
- Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil
Rosana Maria dos Reis, Ph.D.
- Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

Received 12 September 2007; received in revised form 8 November 2007; accepted 27 November 2007. published online 26 February 2008.

Objective

To compare oocyte maturation, fertilization and cleavage rates, and embryonic developmental quality after culture of human immature oocytes from polycystic ovary syndrome (PCOS) patients in human tubal fluid (HTF) or tissue culture medium (TCM) 199.

Design

Prospective, randomized, controlled trial.

Setting

University hospital.

Patient(s)

Thirteen women undergoing 23 in vitro maturation cycles, from whom 119 oocytes were retrieved.

Intervention(s)

Cumulus-enclosed germinal vesicle–stage oocytes matured in TCM-199-supplemented or HTF-supplemented media.

Main Outcome Measure(s)

Oocyte maturation and fertilization rates, embryonic developmental quality.

Result(s)

Significant differences were observed between TCM 199 and HTF regarding maturation rate (82% vs. 56.9%), fertilization rate (70% vs. 39.4%), and embryo quality (81.3% vs. 41.7%).

Conclusion(s)

Human tubal fluid medium, although widely used for embryo fertilization and maintenance in IVF techniques, is not an appropriate medium for the maturation of oocytes obtained from PCOS patients in nonstimulated cycles.

Key Words: Assisted reproductive techniques, in vitro maturation, intracytoplasmic sperm injection, polycystic ovary syndrome, ovarian hyperstimulation syndrome