Freezing and crushing technique: a new concept for the extraction of testicular spermatozoa from men with nonobstructive azoospermia
Ninety samples were harvested from 20 men with nonobstructive azoospermia and divided into two fractions; the first fraction was minced, and the second fraction was exposed to the freezing and crushing (FC) technique. The sperm recovery rate was found to be 21/30 (70%) in the FC fractions compared with 8/30 (26.6%) in the mincing fractions.
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PII: S0015-0282(07)04151-9
doi:10.1016/j.fertnstert.2007.11.087
© 2009 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

