Fertility and Sterility
Volume 91, Issue 2 , Pages 653-655 , February 2009

Freezing and crushing technique: a new concept for the extraction of testicular spermatozoa from men with nonobstructive azoospermia

  • Wael Zohdy, M.D.

      Affiliations

    • Andrology Department, Samir Abbas Medical Center, Al Khobar, Kingdom of Saudi Arabia
    • Cairo University, Cairo, Egypt
    • Corresponding Author InformationReprint requests: Wael Zohdy, M.D., Al Khobar 31952, P.O. Box 3093, Kingdom of Saudi Arabia (FAX: 966-3-8971111).
    • ,
  • Samir Abbas, M.D.

      Affiliations

    • Department of Obstetricians and Gynecologists, Samir Abbas Medical Center, Kingdom of Saudi Arabia
    • ,
  • Ahmed K. Abdel Jalil, M.Sc.

      Affiliations

    • IVF Lab, Samir Abbas Medical Center, Kingdom of Saudi Arabia

Received 31 July 2007 ,Revised 28 November 2007 ,Accepted 28 November 2007.

References 

  1. Nagy Z, Liu J, Joris H, Verheyen G, Tournaye H, Camus M, et al. The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum Reprod. 1995;10:1123–1129
  2. Nagy ZP, Verheyen G, Tournaye H, Devroey P, Van Steirteghem AC. An improved treatment procedure for testicular biopsy specimens offers more efficient sperm recovery: case series. Fertil Steril. 1997;68:376–379
  3. Crabbé E, Verhyen G, Silber S, Tournaye H, Van de Velde H, Goosssens A, et al. Enzymatic digestion of testicular tissue may rescue the intracytoplasmic sperm injection cycle in some patients with non-obstructive azoospermia. Hum Reprod. 1998;13:2791–2796
  4. Aydos K, Demirel LC, Baltaci V, Unlu C. Enzymatic digestion plus mechanical searching improves testicular sperm retrieval in non-obstructive azoospermia cases. Eur J Obstet Gynecol Reprod Biol. 2005;120:80–86
  5. Kamal A, Fahmy I, Mansour RT, Abou-Setta AM, Serour GI, Aboulghar MA. Selection of individual testicular tubules from biopsied testicular tissue with a stereomicroscope improves sperm retrieval rate. J Androl. 2004;25:123–127
  6. Isachenko E, Isachenko V, Katkov I. DNA integrity and motility of human spermatozoa after standard slow freezing versus cryoprotectant-free vitrification. Hum Reprod. 2004;19:932–939
  7. Isachenko V, Isachenko E, Katkov I, Montag M, Dessole S, Nawroth F. Cryoprotectant-free cryopreservation of human spermatozoa by vitrification and freezing in vapor: effect on motility, DNA integrity, and fertlization ability. Bio Reprod. 2004;71:1167–1173

PII: S0015-0282(07)04151-9

doi: 10.1016/j.fertnstert.2007.11.087

Fertility and Sterility
Volume 91, Issue 2 , Pages 653-655 , February 2009

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