Fertility and Sterility
Volume 91, Issue 3 , Pages 878-883 , March 2009

New media for culture to blastocyst

  • Martha Hentemann, M.D.
    • ,
  • Kjell Bertheussen, Ph.D.

      Affiliations

    • Corresponding Author InformationReprint requests: Kjell Bertheussen, Ph.D., In Vitro Fertilization Department, University Hospital of Northern Norway, N-9038 Tromsø, Norway (FAX: 47-77626421).

Received 1 June 2007 ,Revised 28 November 2007 ,Accepted 5 December 2007.

References 

  1. Bertheussen K. Growth of cells in a new defined protein-free medium. Cytotechnology. 1993;11:219–231
  2. Bertheussen K, Forsdahl F, Maltau JM. In vitro fertilization. New media for embryo culture to the blastocyst stage. Proceedings of the 10th World Congress of In Vitro Fertilization and Assisted Reproduction; 1997 May 24–28; Vancouver, British Columbia, Canada: 199–204.
  3. Gardner DK, Lane M. Culture and selection of viable blastocysts: a feasible proposition for human IVF?. Hum Reprod Update. 1997;3:367–382
  4. Quinn P, Horstman FC. Is the mouse a good model for the human with respect to the development of the preimplantation embryo in vitro?. Hum Reprod. 1998;13(Suppl 4):173–183
  5. Gardner DK, Lane M. Alleviation of the “2-cell block” and development to the blastocyst of CF1 mouse embryos: role of amino acids, EDTA and physical parameters. Hum Reprod. 1996;11:2703–2712
  6. Lawitts JA, Biggers JD. Overcoming the 2-cell block by modifying standard components in a mouse embryo culture medium. Biol Reprod. 1991;45:245–251
  7. Dobson AT, Raja R, Abeyta MJ, Taylor T, Shen S, Haqq C, et al. The unique transcriptome through day 3 of human preimplantation development. Hum Mol Genet. 2004;13:1461–1470
  8. Hadi T, Hammer MA, Algire C, Richards T, Baltz JM. Similar effects of osmolarity, glucose, and phosphate on cleavage past the 2-cell stage in mouse embryos from outbred and F1 hybrid females. Biol Reprod. 2005;72:179–187
  9. Gardner DK. Textbook of assisted reproductive techniques. Laboratory and clinical perspectives. London: Taylor and Francis; 2004;
  10. Abramczuk J, Solter D, Koprowski H. The beneficial effect EDTA on development of mouse one-cell embryos in chemically defined medium. Dev Biol. 1977;61:378–383
  11. Biggers JD, McGinnis LK, Lawitts JA. One-step versus two-step culture of mouse preimplantation embryos: is there a difference?. Hum Reprod. 2005;20:3376–3384
  12. Biggers JD, McGinnis L, Summers MC. One-step versus two-step culture of mouse preimplantation embryos. [reply] Hum Reprod. 2006;21:1936–1939
  13. Matsukawa T, Ikeda S, Imai H, Yamada M. Alleviation of the two-cell block of ICR mouse embryos by polyaminocarboxylate metal chelators. Reproduction. 2002;124:65–71
  14. Menezo Y, Chouteau J, Guyader-Joly C, Veiga A. [Sequential media: why and how?]. Contracept Fertil Sex. 1999;27:449–451

 M.H. has nothing to disclose. K.B. is shareholder in Medi-Cult, Denmark.

 Supported in part by MediCult, Jyllinge, Denmark. Dr. Bertheussen is a shareholder in MediCult.

PII: S0015-0282(07)04193-3

doi: 10.1016/j.fertnstert.2007.12.011

Fertility and Sterility
Volume 91, Issue 3 , Pages 878-883 , March 2009

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