Overexpression of fucosyltransferase VII (FUT7) promotes embryo adhesion and implantation
Received 12 July 2007; received in revised form 5 December 2007; accepted 5 December 2007. published online 09 April 2008.
Objective
To investigate the effect of increased fucosyltransferase VII (FUT7) on sialyl Lewis X (sLeX) synthesis and their impact on embryo implantation.
Design
Cell and animal study.
Setting
Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Department of Biochemistry and Molecular Biology, Dalian Medical University.
Animal(s)
Adult female mice of Kunming species.
Intervention(s)
A pIRES2-EGFP-FUT7 expression plasmid containing full-length human FUT7 cDNA was constructed and transiently transfected into RL95-2 or injected into the uteri of early pregnant mice. Control subjects received the mock pIRES2-EGFP or anti-sLeX antibody.
Main Outcome Measure(s)
The expression of FUT7 and sLeX was detected by reverse transcriptase polymerase chain reaction (RT-PCR) and indirect immunofluorescence. The embryo adhesion rate was assayed in vitro with the cocultured RL95-2 and JAR implantation model. In vivo embryo implantation was investigated using a mouse model.
Result(s)
Expression of FUT7 was significantly increased after transfection of pIRES2-EGFP-FUT7 into RL95-2 cells compared with the parental control and mock vector transfectants, as demonstrated by fluorescent microscopy and RT-PCR. Expression of sLeX was also increased in FUT7 overexpressers, as detected by indirect immunofluorescence. Consistent with the elevated expression of FUT7 and sLeX, embryo adhesion rate and embryo implantation competence were significantly increased (P<.01) in both in vitro and in vivo models.
Conclusion(s)
Overexpression of FUT7 up-regulates sLeX synthesis and enhances sLeX-mediated embryo implantation.
aLiaoning Provincial Core Lab of Glycobiology and Glycoengineering, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian, People's Republic of China
bDepartment of Cell Biology, Basic Medical College, Jilin University, Changchun, People's Republic of China
cDepartments of Pediatrics and Dermatology, Children's Memorial Institute for Education and Research, Northwestern University Medical School, Chicago, Illinois
Reprint requests: Qiu Yan, Professor and Director, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116027, People's Republic of China (FAX: 86-411-84722083).
Y.Z. has nothing to disclose. S.L. has nothing to disclose. Y.L. has nothing to disclose. Z.W. has nothing to disclose. X.W. has nothing to disclose. Q.Y. has nothing to disclose.
Supported by the National Natural Science Foundation of China (nos. 30270329 and 30670465).
ABSTRACT