Fertility and Sterility
Volume 91, Issue 3 , Pages 920-924, March 2009

Blastocyst rate and live births from vitrification and slow-cooled two-cell mouse embryos

  • Jennifer E. Graves-Herring, M.S.
    • ,
  • William R. Boone, Ph.D.

      Affiliations

    • Corresponding Author InformationReprint requests: William R. Boone, Ph.D., University Medical Group, Greenville Hospital System, 890 W. Faris Road, Suite 470, Greenville, South Carolina, 29605 (FAX: 864-455-8492).

Department of Obstetrics and Gynecology, Division of Reproduction Endocrinology and Infertility, Greenville Hospital System University Medical Group, Greenville, South Carolina

Received 22 June 2007; received in revised form 11 December 2007; accepted 11 December 2007. published online 04 March 2008.

Objective

The purpose of this study was to develop a closed vitrification system, compare vitrification to a slow-cooled cryopreservation method, and compare the pup rate between both methods using two-cell mouse embryos.

Design

Randomized, prospective animal study.

Setting

Hospital-based IVF practice.

Animal(s)

B6C3F1 mouse embryos.

Intervention(s)

Two-cell mouse embryos were cryopreserved using a slow-cooled or vitrification method and then thawed at a later date. The embryos were cultured and transferred to recipient females.

Main Outcome Measure(s)

Embryos were observed for blastocyst rate and pups were observed for phenotypic anomalies and weighed at 30, 60, and 90 days after birth.

Result(s)

Neither the blastocyst rate, pup rate, nor pup weights were significantly different when the two cryopreservation methods were compared.

Conclusion(s)

Because there were no differences in blastocyst rates, pup rates, or pup weights, we plan to further investigate the potential effects of vitrification on genotypic damage via the Comet Assay.

Key Words: Vitrification, slow cooled, cryopreservation, embryos, live births

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

 There is no conflict of interest, financial or otherwise, for either of the submitting authors.

 Funding was provided by Greenville Hospital System University Medical Group, Department of Obstetrics and Gynecology, Division of Reproduction Endocrinology and Infertility, Greenville, South Carolina.

PII: S0015-0282(07)04329-4

doi:10.1016/j.fertnstert.2007.12.045

Fertility and Sterility
Volume 91, Issue 3 , Pages 920-924, March 2009

Article Tools