High expression levels of cyclin B1 and Polo-like kinase 1 in ectopic endometrial cells associated with abnormal cell cycle regulation of endometriosis
Received 29 July 2007; received in revised form 8 January 2008; accepted 11 January 2008. published online 18 March 2008.
Objective
To investigate the possible roles of cyclin B1/cyclin-dependent kinase (cdc2) and Polo-like kinase 1 (Plk1) in the pathogenesis of endometriosis.
Design
A case-control study.
Setting
University hospital.
Patient(s)
Patients with or without endometriosis were diagnosed by pathological examination or laparoscopy. The patients with the following criteria within the past 6 months were excluded: endocrine or inflammatory diseases, pregnancy or lactation, hormonal therapy, and neoplasm in the uterine cavity.
Intervention(s)
Eutopic and ectopic endometria were obtained at the time of surgery. Blood was collected on the same day as surgery.
Main Outcome Measure(s)
The mRNA/protein expression and localization of cyclin B1, cdc2, and Plk1 in endometrium, and serum levels of E2 and P.
Result(s)
The expression levels of cyclin B1 and Plk1, but not cdc2, in ectopic endometria were significantly higher than in eutopic endometria. The immunohistochemical staining of cyclin B1 and Plk1 was detected in the nuclei of ectopic and eutopic endometrial cells. Furthermore, ectopic endometrial expression levels of cyclin B1 or Plk1 were positively correlated with serum E2 levels.
Conclusion(s)
Cyclin B1 and Plk1 may play important roles in the pathogenesis of endometriosis by mediating ectopic endometrial cell proliferation under regulation of ovarian hormones.
aDepartment of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China
bDepartment of Reproductive Endocrinology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, People's Republic of China
Reprint requests: He-Feng Huang, M.D., Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 2 Xue Shi Road, Hangzhou, Zhejiang, 310006, People's Republic of China (FAX: 86-571-88208022).
L.T. has nothing to disclose. T.-T.W. has nothing to disclose. Y.-T.W. has nothing to disclose. C.-Y.Z. has nothing to disclose. H.-F.H. has nothing to disclose.
Li Tang and Ting-Ting Wang contributed equally to this study.
Supported by the National Natural Science Foundation of China (30670789), National Basic Research Program of China (2006CB944006, 2006CB504004), Key Research Program of Zhejiang Province (2006C13078), and Key Research Program of Education of Zhejiang Province (20061382).
ABSTRACT