Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients
Received 10 October 2007; received in revised form 25 January 2008; accepted 25 January 2008. published online 25 April 2008.
Objective
To investigate DNA fragmentation by using terminal deoxyribonucleotidyl transferase–mediated dUTP nick-end labeling in relation to World Health Organization parameters and computer-aided sperm analysis (CASA) in sperm to determine the possibility of obtaining a correlation among CASA parameters, sperm morphology, and DNA fragmentation.
Design
Sperm analysis according to World Health Organization parameters, terminal deoxyribonucleotidyl transferase–mediated dUTP nick-end labeling (TUNEL) for sperm DNA fragmentation, and CASA for sperm movement. Prospective study.
Setting
All the patients were under clinical management, consulting for hypofertility at a fertility center in France.
Patient(s)
One thousand six hundred thirty-three men who were referred for infertility investigation, including a complete sperm analysis.
Intervention(s)
Sperm analysis and DNA damage testing.
Main Outcome Measure(s)
Sperm morphology, DNA fragmentation, and movement characteristics.
Result(s)
One third of the patients had a TUNEL rate of >30%. Analysis of the 21 semen parameters tested revealed that 7 of them were significantly correlated with the TUNEL results.
Conclusion(s)
World Health Organization sperm parameters and DNA damage are complementary, rather than strongly linked. This should be considered to more fully understand the paternal contribution in assisted reproductive technologies failures.
Reprint requests: Paul Cohen-Bacrie, Dr.Pharm., Laboratoire d'Eylau, 55 rue Saint Didier, 75116, Paris, France (FAX: 33957799422).
P.C.-B. has nothing to disclose. S.B. has nothing to disclose. Y.J.R.M. has nothing to disclose. P.C. has nothing to disclose. J.H. has nothing to disclose. M.B. has nothing to disclose.
ABSTRACT