Fertility and Sterility
Volume 91, Issue 5 , Pages 1668-1675 , May 2009

Iron storage is significantly increased in peritoneal macrophages of endometriosis patients and correlates with iron overload in peritoneal fluid

  • Jean-Christophe Lousse, M.D.

      Affiliations

    • Department of Gynecology, Université Catholique de Louvain, Brussels, Belgium
    • ,
  • Sylvie Defrère, Ph.D.

      Affiliations

    • Department of Gynecology, Université Catholique de Louvain, Brussels, Belgium
    • ,
  • Anne Van Langendonckt, Ph.D.

      Affiliations

    • Department of Gynecology, Université Catholique de Louvain, Brussels, Belgium
    • ,
  • Jérémie Gras, M.D.

      Affiliations

    • Department of Clinical Biology, Université Catholique de Louvain, Brussels, Belgium
    • ,
  • Reinaldo González-Ramos, M.D., Ph.D.

      Affiliations

    • Department of Gynecology, Université Catholique de Louvain, Brussels, Belgium
    • ,
  • Sébastien Colette, M.Sc.

      Affiliations

    • Department of Gynecology, Université Catholique de Louvain, Brussels, Belgium
    • ,
  • Jacques Donnez, M.D., Ph.D.

      Affiliations

    • Department of Gynecology, Université Catholique de Louvain, Brussels, Belgium
    • Corresponding Author InformationReprint requests: Jacques Donnez, M.D., Ph.D., Department of Gynecology, Université Catholique de Louvain, Cliniques Universitaires St Luc, Avenue Hippocrate 10, 1200 Brussels, Belgium (FAX: +32-2-764-95-07).

Received 23 October 2007 ,Revised 11 February 2008 ,Accepted 11 February 2008.

References 

  1. Giudice LC, Kao LC. Endometriosis. Lancet. 2004;364:1789–1799
  2. Halme J, Hammond MG, Hulka JF, Raj SG, Talbert LM. Retrograde menstruation in healthy women and in patients with endometriosis. Obstet Gynecol. 1984;64:151–154
  3. Kruitwagen RF. Menstruation as the pelvic aggressor. Bailliere Clin Obstet Gynaecol. 1993;7:687–700
  4. Sampson JA. Peritoneal endometriosis due to the menstrual dissemination of endometrial tissue into the peritoneal cavity. Am J Obstet Gynecol. 1927;14:422–469
  5. Dunselman . Peritoneal environment in endometriosis. In:  Shaw RW editors. Endometriosis: current understanding and management. Oxford: Blackwell Science; 1995;p. 47–74
  6. Vinatier D, Cosson M, Dufour P. Is endometriosis an endometrial disease?. Eur J Obstet Gynecol Reprod Biol. 2000;91:113–125
  7. Halme J, Becker S, Wing R. Accentuated cyclic activation of peritoneal macrophages in patients with endometriosis. Am J Obstet Gynecol. 1984;148:85–90
  8. Oral E, Olive DL, Arici A. The peritoneal environment in endometriosis. Hum Reprod Update. 1996;2:385–398
  9. Knutson M, Wessling-Resnick M. Iron metabolism in the reticuloendothelial system. Crit Rev Biochem Mol Biol. 2003;38:61–88
  10. Ganz T. Hepcidin—a regulator of intestinal iron absorption and iron recycling by macrophages. Best Pract Res Clin Haematol. 2005;18:171–182
  11. Peyssonnaux C, Zinkernagel AS, Datta V, Lauth X, Johnson RS, Nizet V. TLR4-dependent hepcidin expression by myeloid cells in response to bacterial pathogens. Blood. 2006;107:3727–3732
  12. Nguyen NB, Callaghan KD, Ghio AJ, Haile DJ, Yang F. Hepcidin expression and iron transport in alveolar macrophages. Am J Physiol Lung Cell Mol Physiol. 2006;291:417–425
  13. Arumugam K. Endometriosis and infertility: raised iron concentration in the peritoneal fluid and its effect on the acrosome reaction. Hum Reprod. 1994;9:1153–1157
  14. Arumugam K, Yip YC. De novo formation of adhesions in endometriosis. The role of iron and free radical reactions. Fertil Steril. 1995;64:62–64
  15. Van Langendonckt A, Casanas-Roux F, Donnez J. Iron overload in the peritoneal cavity of women with pelvic endometriosis. Fertil Steril. 2002;78:712–718
  16. Martinez-Roman S, Balasch J, Creus M, Fabregues F, Carmona F, Vilella R, et al. Transferrin receptor (CD71) expression in peritoneal macrophages from fertile and infertile women with and without endometriosis. Am J Reprod Immunol. 1997;38:413–417
  17. Mathur SP, Lee JH, Jiang H, Arnaud P, Rust PF. Levels of transferrin and alpha 2-HS glycoprotein in women with and without endometriosis. Autoimmunity. 1999;29:121–127
  18. Van Langendonckt A, Casanas-Roux F, Donnez J. Oxidative stress and peritoneal endometriosis. Fertil Steril. 2002;77:861–870
  19. Ward RJ, Wilmet S, Legssyer R, Crichton RR. The influence of iron homoeostasis on macrophage function. Biochem Soc Trans. 2002;30:762–765
  20. Lousse JC, Van Langendonckt A, González Ramos R, Defrère S, Renkin E, Donnez J. Increased activation of nuclear factor-kappa B (NF-κB) in isolated peritoneal macrophages of patients with endometriosis. Fertil Steril. Published online September 21, 2007.
  21. American Society for Reproductive Medicine. Revised American Society for Reproductive Medicine classification of endometriosis: 1996. Fertil Steril. 1997;67:817–821
  22. Rahier J, Stevens M, de Menten Y, Henquin JC. Determination of antigen concentration in tissue sections by immunodensitometry. Lab Invest. 1989;61:357–363
  23. Boyden A, Button E, Germerog D. Precipitin testing with special reference to the measurement of turbidity. J Immunol. 1947;57:211
  24. Addison GM, Beamish MR, Hales CN, Hodgkins M, Jacobs A, Llewellin P. An immunoradiometric assay for ferritin in the serum of normal subjects and patients with iron deficiency and iron overload. J Clin Pathol. 1972;25:326–329
  25. Defrère S, Van Langendonckt A, Vaesen S, Jouret M, González Ramos R, Gonzalez D, et al. Iron overload enhances epithelial cell proliferation in endometriotic lesions induced in a murine model. Hum Reprod. 2006;21:2810–2816
  26. D'Hooghe TM, Debrock S. Endometriosis, retrograde menstruation and peritoneal inflammation in women and in baboons. Hum Reprod Update. 2002;8:84–88
  27. Nisolle M, Casanas-Roux F, Wyns C, de Menten Y, Mathieu PE, Donnez J. Immunohistochemical analysis of estrogen and progesterone receptors in endometrium and peritoneal endometriosis: a new quantitative method. Fertil Steril. 1994;62:751–759
  28. Hippeli S, Elstner EF. Transitional metal ion-catalyzed oxygen activation during pathogenic processes. FEBS Lett. 1999;443:1–7
  29. Balla G, Jacob HS, Balla J, Rosenberg M, Nath K, Apple F, et al. Ferritin: a cytoprotective antioxidant stratagem of endothelium. J Biol Chem. 1992;267:18148–18153
  30. Santanam N, Murphy AA, Parthasarathy S. Macrophages, oxidation, and endometriosis. Ann NY Acad Sci. 2002;955:183–198
  31. Guo SW. Nuclear factor-κB (NF-κB): an unsuspected major culprit in the pathogenesis of endometriosis that is still at large?. Gynecol Obstet Invest. 2007;63:71–97
  32. González-Ramos R, Donnez J, Defrère S, Leclercq I, Squifflet J, Lousse JC, et al. Nuclear factor-kappa B (NF-κB) is constitutively activated in peritoneal endometriosis. Mol Hum Reprod. 2007;13:503–509
  33. Wieser F, Vigne JL, Ryan I, Hornung D, Djalali S, Taylor RN. Sulindac suppresses nuclear factor-kappaB activation and RANTES gene and protein expression in endometrial stromal cells from women with endometriosis. J Clin Endocrinol Metab. 2005;90:6441–6447
  34. Viatour P, Merville MP, Bours V, Chariot A. Phosphorylation of NF-κB and IκB proteins: implications in cancer and inflammation. Trends Biochem Sci. 2005;30:43–52
  35. Sakaeda Y, Hiroi M, Shimojima T, Iguchi M, Kanegae H, Ohmori Y. Sulindac, a nonsteroidal anti-inflammatory drug, selectively inhibits interferon-γ-induced expression of the chemokine CXCL9 gene in mouse macrophages. Biochem Biophys Res Commun. 2006;350:339–344

 Supported by grants from the Fonds de la Recherche Scientifique (FRS)–FNRS, Belgium (No. 1.5.010.06), La Région Wallonne, Vicomte Ph. de Spoelberch, and Baron A. Frère.

 J-C.L. is an FRS-FNRS Research Fellow and has nothing to disclose. S.D. has nothing to disclose. A.V. has nothing to disclose. J.G. has nothing to disclose. R.G-R. has nothing to disclose. S.C. has nothing to disclose. J.D. has nothing to disclose.

PII: S0015-0282(08)00393-2

doi: 10.1016/j.fertnstert.2008.02.103

Fertility and Sterility
Volume 91, Issue 5 , Pages 1668-1675 , May 2009

Article Tools

* Image Usage & Resolution