Fertility and Sterility
Volume 91, Issue 5 , Pages 1785-1792 , May 2009

Coenzyme Q10 treatment in infertile men with idiopathic asthenozoospermia: a placebo-controlled, double-blind randomized trial

  • Giancarlo Balercia, M.D.

      Affiliations

    • Andrology Unit, Endocrinology, Department of Internal Medicine and Applied Biotechnologies, Umberto I Hospital, School of Medicine, Polytechnic University of Marche, Ancona, Italy
    • Corresponding Author InformationReprint requests: Giancarlo Balercia, M.D., Andrology Unit, Endocrinology, Department of Internal Medicine and Applied Biotechnologies, Umberto I Hospital, Torrette, Via Conca, 60100 Ancona, Italy (FAX: 39-071-887-300).
    • ,
  • Eddi Buldreghini, Ph.D.

      Affiliations

    • Andrology Unit, Endocrinology, Department of Internal Medicine and Applied Biotechnologies, Umberto I Hospital, School of Medicine, Polytechnic University of Marche, Ancona, Italy
    • ,
  • Arianna Vignini, Ph.D.

      Affiliations

    • Institute of Biochemistry, School of Medicine, Polytechnic University of Marche, Ancona, Italy
    • ,
  • Luca Tiano, Ph.D.

      Affiliations

    • Institute of Biochemistry, School of Medicine, Polytechnic University of Marche, Ancona, Italy
    • ,
  • Francesca Paggi, M.D.

      Affiliations

    • Andrology Unit, Endocrinology, Department of Internal Medicine and Applied Biotechnologies, Umberto I Hospital, School of Medicine, Polytechnic University of Marche, Ancona, Italy
    • ,
  • Salvatore Amoroso, M.D.

      Affiliations

    • Department of Neuroscience, Unit of Pharmacology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
    • ,
  • Giuseppe Ricciardo-Lamonica, Ph.D.

      Affiliations

    • Department of Economics, School of Economics, Polytechnic University of Marche, Ancona, Italy
    • ,
  • Marco Boscaro, M.D.

      Affiliations

    • Andrology Unit, Endocrinology, Department of Internal Medicine and Applied Biotechnologies, Umberto I Hospital, School of Medicine, Polytechnic University of Marche, Ancona, Italy
    • ,
  • Andrea Lenzi, M.D.

      Affiliations

    • Andrology, Pathophysiology of Reproduction and Endocrine Diagnosis Unit, Policlinico Umberto I, University of Rome “La Sapienza,” Rome, Italy
    • ,
  • GianPaolo Littarru, M.D.

      Affiliations

    • Institute of Biochemistry, School of Medicine, Polytechnic University of Marche, Ancona, Italy

Received 12 December 2007 ,Revised 4 February 2008 ,Accepted 12 February 2008.

References 

  1. Alvarez JG, Storey B. Spontaneous lipid peroxidation in rabbit epididymal spermatozoa: its effect on sperm motility. Biol Reprod. 1982;27:1102–1108
  2. Aitken RJ, Clarkson JS. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Reprod Fertil. 1987;81:459–469
  3. Aitken RJ, Clarkson JS, Fishel S. Generation of reactive oxygen species, lipid peroxidation and human sperm function. Biol Reprod. 1989;40:183–197
  4. Rao B, Soufir JC, Martin M, David G. Lipid peroxidation in human spermatozoa as related to midpiece abnormalities and motility. Gamete Res. 1989;24:127–134
  5. Suleiman SA, Ali ME, Zaki ZM, El-Malik EM, Nasr MA. Lipid peroxidation and human sperm motility: protective role of vitamin E. J Androl. 1996;17:530–537
  6. Aitken RJ, Krausz C. Oxidative stress, DNA damage and the Y chromosome. Reproduction. 2001;122:497–506
  7. Aitken RJ, Baker MA. Reactive oxygen species generation by human spermatozoa: a continuing enigma. Int J Androl. 2002;25:191–194
  8. Balercia G, Moretti S, Vignini A, Magagnini M, Mantero F, Boscaro M, et al. Role of nitric oxide concentration on human sperm motility. J Androl. 2004;25:245–249
  9. Agarwal A, Said MT, Bedaiwy MA, Banerjee J, Alvarez JG. Oxidative stress in assisted reproductive techniques setting. Fertil Steril. 2006;86:503–512
  10. Agarwal A, Prabakaran S, Allamaneni S. What an andrologist/urologist should know about free radicals and why. Urology. 2006;67:2–8
  11. Karlsson J. Heart and skeletal muscle ubiquinone or CoQ10 as protective agent against radical formation in man. Adv Myochem. 1987;1:305–308
  12. Ernster L, Forsmark-Andrée P. Ubiquinol: an endogenous antioxidant in aerobic organisms. Clin Invest. 1993;71:60–65
  13. Groneberg D, Kindermann B, Althammer M, Klapper M, Vormann J, Littarru GP, et al. Coenzyme Q10 affects expression of genes involved in cell signalling, metabolism and transport in human CaCo-2 cells. Int J Biochem Cell Biol. 2005;37:1208–1218
  14. Kalèn A, Appelkvist EL, Chojnaki T, Dallner G. Nonaprenyl-4-hydroxibenzoate transferase, an enzyme involved in ubiquinone biosynthesis in endoplasmic reticulum–Golgi system. J Biol Chem. 1990;25:1158–1164
  15. Mancini A, De Marinis L, Oradei A, Hallgass E, Conte G, Pozza D, et al. Coenzyme Q10 concentration in normal and pathological human seminal fluid. J Androl. 1994;15:591–594
  16. Alleva R, Tomassetti M, Battino M, Curatola G, Littarru G, Folkers K. Role of CoQ10 in preventing peroxidation of LDL subfraction. Proc Natl Acad Sci U S A. 1995;92:9388–9391
  17. Mancini A, Conte G, Milardi D, De Marinis L, Littarru G. Relationship between sperm cell ubiquinone and seminal parameters in subjects with and without varicocele. Andrologia. 1998;30:1–4
  18. Balercia G, Arnaldi G, Fazioli F, Serresi M, Alleva R, Mancini A, et al. Coenzyme Q10 levels in idiopathic and varicocele-associated asthenozoospermia. Andrologia. 2002;34:107–111
  19. Balercia G, Mantero F, Armeni T, Principato G, Regoli F. Oxyradical scavenging capacity toward different reactive species in seminal plasma and sperm cells. A possible influence on kinetic parameters. Clin Chem Lab Med. 2003;41:13–19
  20. Mancini A, De Marinis L, Littarru GP, Balercia G. An update of coenzyme Q10 implications in male infertility: biochemical and therapeutic aspects. Biofactors. 2005;25:165–174
  21. Balercia G, Mosca F, Mantero F, Boscaro M, Mancini A, Ricciardo-Lamonica G, et al. Coenzyme Q10 supplementation in infertile men with idiopathic asthenozoospermia: an open, uncontrolled pilot study. Fertil Steril. 2004;81:93–98
  22. World Health Organization . Laboratory manual for examination of semen and semen–cervical mucus interaction. 4th ed.. New York: Cambridge University Press; 1999;
  23. Alleva R, Scaramucci A, Mantero F, Bompadre S, Leone L, Littarru G. Protective role of ubiquinol content against formation of lipid hydroperoxide in human seminal fluid. Molec Aspect Med. 1997;18:S221–S228
  24. Sekine K, Ota N, Nishii M, Uetake T, Shimadzu M. Estimation of plasma and saliva levels of coenzyme Q10 and influence of oral supplementation. Biofactors. 2005;25:205–211
  25. Lewis S, Boyle P, McKinney MB, Young I, Thompson W. Total antioxidant capacity of seminal plasma is different in fertile and infertile men. Fertil Steril. 1995;64:868–870
  26. Rhemrev JP, Van Overveld FW, Haenen GR, Teerlink T, Bast A, Vermeiden JP. Quantification of the nonenzymatic fast and slow TRAP in a postaddition assay in human seminal plasma and the antioxidant contribution of various seminal compounds. J Androl. 2000;21:913–920
  27. Moncada ML, Vicari E, Cimino C, Calogero AE, Mongioì A, D'Agata R. Effect of acetylcarnitine treatment in oligoasthenospermic patients. Acta Eur Fertil. 1992;23:221–224
  28. Lenzi A, Culasso F, Gandini L, Lombardo F, Dondero F. Placebo-controlled, double blind, cross-over trial of glutathione therapy in male infertility. Hum Reprod. 1993;8:1657–1662
  29. Costa M, Canale D, Filicori M, D'Iddio S, Lenzi A. L-Carnitine in idiopathic asthenozoospermia: a multicenter study. Andrologia. 1994;26:155–159
  30. Sikka SC, Rajasekaran M, Hellstrom WJG. Role of oxidative stress and antioxidant in male infertility. J Androl. 1995;16:464–468
  31. Vitali G, Parente R, Melotti C. Carnitine supplementation in human idiopathic asthenozoospermia: clinical results. Drugs Exp Clin Res. 1995;21:157–159
  32. Ford WCL, Whittington K. Antioxidant treatment for male subfertility: a promise that remains unfulfilled. Hum Reprod. 1998;13:1416–1419
  33. Geva E, Lessing JB, Lerner-Geva L, Amit A. Free radicals, antioxidants and human spermatozoa: clinical implications. Hum Reprod. 1998;13:1415–1424
  34. Lenzi A, Gandini L, Picardo M. A rationale for glutathione therapy. Hum Reprod. 1998;13:1419–1421
  35. Tarin JJ, Brines J, Cano A. Antioxidants may protect against infertility. Hum Reprod. 1998;13:1415–1416
  36. Comhaire FH, Mahmoud AM, Depuydt CE, Zalata AA, Christophe AB. Mechanisms and effects of male genital tract infections on semen quality and fertilizing potential: the andrologist's viewpoint. Hum Reprod Update. 1999;5:393–398
  37. Lenzi A, Lombardo F, Sgro P, Salacone P, Caponecchia L, Dondero F, et al. Use of carnitine therapy in selected cases of male factor infertility: a double blind cross-over trial. Fertil Steril. 2003;79:292–300
  38. Lenzi A, Sgrò P, Salacone P, Paoli D, Gilio B, Lombardo F, et al. Placebo controlled double blind randomized trial on the use of L-carnitine and L-acetyl-carnitine combined treatment in asthenozoospermia. Fertil Steril. 2004;81:1578–1584
  39. Balercia G, Regoli F, Armeni T, Koverech A, Mantero F, Boscaro M. Placebo-controlled double-blind randomized trial on the use of L-carnitine, L-acetylcarnitine, or combined L-carnitine and L-acetylcarnitine in men with idiopathic asthenozoospermia. Fertil Steril. 2005;84:662–671
  40. Vicari E, La Vignera S, Calogero AE. Antioxidant treatment with carnitine is effective in infertile patients with prostato-vesiculo-epididymitis and elevated seminal leukocyte concentration after treatment with nonsteroidal anti-inflammatory compounds. Fertil Steril. 2002;78:1203–1208
  41. Stocker R, Bowry VW, Frei B. Ubiquinol-10 protects human low density lipoprotein more efficiently against lipid peroxidation than does α-tocopherol. Proc Natl Acad Sci U S A. 1991;88:1646–1650
  42. Mancini A, Milardi D, Conte G, Bianch A, Balercia G, De Marinis L, et al. Coenzyme Q10: another biochemical alteration linked to infertility in varicocele patients?. Metabolism. 2003;52:402–406
  43. Fato R, Cavazzoni M, Castelluccio C, Parenti Castelli G, Lenaz G. Steady-state kinetics of ubiquinol–cytochrome c reductase in bovine heart submitochondrial particles: diffusional effects. Biochem J. 1993;290:225–236

 G.B. has nothing to disclose. E.B. has nothing to disclose. A.V. has nothing to disclose. L.T. has nothing to disclose. F.P. has nothing to disclose. S.A. has nothing to disclose. G.R.-L. has nothing to disclose. M.B. has nothing to disclose. A.L. has nothing to disclose. G.L. has nothing to disclose.

PII: S0015-0282(08)00487-1

doi: 10.1016/j.fertnstert.2008.02.119

Fertility and Sterility
Volume 91, Issue 5 , Pages 1785-1792 , May 2009

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