Pregnancy after trophectoderm biopsy of frozen–thawed blastocyst
Received 29 December 2007; received in revised form 20 February 2008; accepted 20 February 2008. published online 27 March 2008.
Objective
To report a case of a successful pregnancy after trophectoderm biopsy and three-probe fluorescent in situ hybridization of a frozen blastocyst.
Design
Techniques and instrumentation.
Setting
A University Medical Center.
Patient(s)
Infertility patient desiring trophectoderm biopsy on frozen blastocyst for preimplantation testing, from an IVF cycle at a referring IVF program.
Intervention(s)
Frozen blastocysts were thawed the evening before the planned transfer. Trophectoderm biopsy was performed in the morning. The fluorescent in situ hybridization results were obtained the same day; embryo transfer was performed under ultrasound guidance.
Main Outcome Measurement(s)
Serum βhCG and transvaginal ultrasound.
Result(s)
Positive βhCG and ongoing pregnancy.
Conclusion(s)
Trophectoderm biopsy can be used as a means for testing frozen blastocysts in patients with excess embryos cryopreserved on day 5 or 6 from previously preformed IVF cycles.
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Stanford University Medical Center, Stanford, California
Reprint requests: Ruth Lathi, M.D., Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford University, 300 Pasteur Drive, Stanford, CA 94305 (FAX: 650-723-7737).
R.B.L. has nothing to declare. B.B. has nothing to declare.
ABSTRACT