Pregnancy after trophectoderm biopsy of frozen–thawed blastocyst

Objective

To report a case of a successful pregnancy after trophectoderm biopsy and three-probe fluorescent in situ hybridization of a frozen blastocyst.

Design

Techniques and instrumentation.

Setting

A University Medical Center.

Patient(s)

Infertility patient desiring trophectoderm biopsy on frozen blastocyst for preimplantation testing, from an IVF cycle at a referring IVF program.

Intervention(s)

Frozen blastocysts were thawed the evening before the planned transfer. Trophectoderm biopsy was performed in the morning. The fluorescent in situ hybridization results were obtained the same day; embryo transfer was performed under ultrasound guidance.

Main Outcome Measurement(s)

Serum βhCG and transvaginal ultrasound.

Result(s)

Positive βhCG and ongoing pregnancy.

Conclusion(s)

Trophectoderm biopsy can be used as a means for testing frozen blastocysts in patients with excess embryos cryopreserved on day 5 or 6 from previously preformed IVF cycles.

Key Words: Trophectoderm biopsy, cryopreserved embryo, frozen embryo, blastocyst, frozen blastocyst, PGD, PGS