Fertility and Sterility
Volume 91, Issue 6 , Pages 2461-2465 , June 2009

Low-oxygen compared with high-oxygen atmosphere in blastocyst culture, a prospective randomized study

  • Urban Waldenström, M.D., Ph.D.

      Affiliations

    • In Vitro Fertilization Unit, Falun Hospital, Falun, Sweden
    • Corresponding Author InformationReprint requests: Urban Waldenström, IVF Unit, Falun Hospital, S-79182 Falun, Sweden (FAX: +46-23-490763).
    • ,
  • Ann-Britt Engström, B.App.Sc.

      Affiliations

    • In Vitro Fertilization Unit, Falun Hospital, Falun, Sweden
    • ,
  • Dan Hellberg, M.D., Ph.D.

      Affiliations

    • Center for Clinical Research, Falun, Sweden
    • Department of Women's and Children's Health, Uppsala, Sweden
    • ,
  • Staffan Nilsson, M.D., Ph.D.

      Affiliations

    • In Vitro Fertilization Unit, Falun Hospital, Falun, Sweden
    • Center for Clinical Research, Falun, Sweden
    • Department of Women's and Children's Health, Uppsala, Sweden

Received 14 June 2007 ,Revised 18 March 2008 ,Accepted 18 March 2008.

References 

  1. Menezo Y, Veiga A, Benkhalifa M. Improved methods for blastocyst formation and culture. Hum Reprod. 1998;13(Suppl 4):256–265
  2. Gardner DK, Lane M. Culture and selection of viable blastocysts: a feasible proposition for IVF?. Hum Reprod Update. 1997;3:367–382
  3. Nilsson S, Waldenström U, Engström A-B, Hellberg D. Promising results with 306 single blastocyst transfers. Fertil Steril. 2005;83:1849–1851
  4. Papanikolaou EG, Camus M, Kolibianakis EM, Van Landuyt L, Van Steirteghem A, Devroey P. In vitro fertilization with single blastocyst-stage versus single cleavage-stage embryos. New Engl J Med. 2006;354:1139–1146
  5. Sakkas D, Gardner DK. Noninvasive methods to assess embryo quality. Curr Opinion Obstet Gynecol. 2005;17:283–288
  6. Langley MT, Marek DM, Gardner DK, Doody KM, Doody KJ. Extended embryo culture in human assisted reproduction. Hum Reprod. 2001;16:902–908
  7. Shapiro B, Richter KS, Harris DC, Daneshmand S. Influence of patient age on the growth and transfer of blastocyst-stage embryos. Fertil Steril. 2002;77:700–705
  8. Wilson M, Hartke K, Kiehl M, Rodgers J, Brabec C, Lyles R. Transfer of blastocysts and morulae on day 5. Fertil Steril. 2004;82:327–333
  9. Trokoudes KM, Minbattiwalla MB, Kalogirou L, Pantelides K, Mitsingas P, Sokratous A, et al. Controlled natural cycle IVF with antagonist use and blastocyst transfer. Reprod Biomed Online. 2005;11:685–689
  10. Guerif F, Le Gouge A, Giraudeau B, Poindron J, Bidault R, Gasnier O, et al. Limited value of morphological assessment at days 1 and 2 to predict blastocyst development potential: a prospective study on 4042 embryos. Hum Reprod. 2007;22:1973–1981
  11. Fujitani Y, Kasai K, Ohtani S, Nishimura K, Yamada M, Utsumi K. Effect of oxygen concentration and free radicals on in vitro development of in vitro-produced bovine embryos. J Anim Sci. 1997;75:483–489
  12. Booth PJ, Holm P, Callesen H. The effect of oxygen tension on porcine embryonic development is dependent on embryo type. Theriogenology. 2004;63:2040–2052
  13. Waldenström U, Hellberg D, Nilsson S. Low-dose aspirin in a short regimen as standard treatment in in vitro fertilization—a randomized, prospective study. Fertil Steril. 2004;81:1560–1564
  14. Schoolcraft WB, Gardner DK, Lane M, Schlenker T, Hamilton F, Meldrum DR. Blastocyst culture and transfer: analysis of results and parameters affecting outcome in two in vitro fertilization programs. Fertil Steril. 1999;72:604–609
  15. Takahashi Y, Hishinuma M, Matsui M, Tanaka H, Kanagawa H. Development of in vitro matured/fertilized bovine embryos in a chemically defined medium: influence of oxygen concentration in the gas atmosphere. J Vet Med Sci. 1996;58:897–902
  16. Noda Y, Goto Y, Umaoka Y, Shiotani M, Nakayama T, Mori T. Culture of human embryos in alpha modification of Eagle's medium under low oxygen tension and low illumination. Fertil Steril. 1994;62:1022–1027
  17. Dalvit GC, Cetica PD, Pintos LN, Beconi MT. Reactive oxygen species in bovine embryo in vitro production. Biocell. 2005;29:209–212
  18. Feichtinger W, Kemeter P, Szalay S. The Vienna program of in vitro fertilization and embryo transfer—a successful clinical treatment. Eur J Obstet Gynecol Reprod Biol. 1983;15:63–70
  19. Dumoulin JCM, Vanvuchelen RCM, Land JA, Pieters MHEC, Geraedts JPM, Evers JLH. Effect of oxygen concentration on in vitro fertilization and embryo culture in the human and the mouse. Fertil Steril. 1995;63:115–119
  20. Dumoulin JCM, Meijers CJJ, Bras M, Coonen E, Gearedts JPM, Evers JLH. Effect of oxygen concentration on human in-vitro fertilization and embryo culture. Hum Reprod. 1999;14:465–469
  21. Behr B, Pool TB, Milki AA, Moore D, Gebhardt J, Dasig D. Preliminary clinical experience with human blastocyst development in vitro without co-culture. Hum Reprod. 1999;14:454–457
  22. Gardner DK, Lane M, Johnson J, Wagley L, Stevens J, Schoolcraft WB. Reduced oxygen tension increases blastocyst development, differentiation and viability. [abstract] Fertil Steril. 1999;72(Suppl 1):S30–S31
  23. Kea B, Gebhardt J, Watt J, Westphal LM, Lathi RB, Milki AA, et al. Effect of reduced oxygen concentrations on the outcome of in vitro fertilization. Fertil Steril. 2007;87:213–216
  24. Adam AA, Takahashi Y, Katagiri S, Nagano M. Effects of oxygen tension in the gas atmosphere during in vitro maturation, in vitro fertilization and in vitro culture on the efficiency of in vitro production of mouse embryos. Jpn J Vet Res. 2004;52:77–84
  25. Quinn P, Harlow GM. The effect of oxygen on the development of preimplantation mouse embryos in vitro. J Exp Zool. 1978;206:73–80
  26. Harlow GM, Quinn P. Foetal and placental growth in the mouse after pre-implantation development in vitro under oxygen concentrations of 5 and 20%. Aust J Biol Sci. 1979;32:363–369
  27. Gardner DK, Lane M. Alleviation of the “2-cell block” and development to the blastocyst of CF1 mouse embryos: role of amino acids, EDTA and physical parameters. Hum Reprod. 1996;11:2703–2712
  28. Johnston LA, Donoghue AM, O'Brien SJ, Wildt DE. Influence of temperature and gas atmosphere on in-vitro fertilization and embryo development in domestic cats. J Reprod Fertil. 1991;92:377–382
  29. Batt PA, Gardner DK, Cameron AW. Oxygen concentration and protein source affect the development of preimplantation goat embryos in vitro. Reprod Fertil Dev. 1991;3:601–607
  30. Thompson JG, Simpson AC, Pugh PA, Donnely PE, Tervit HR. Effect of oxygen concentration on in vitro-development of preimplantation sheep and cattle embryos. J Reprod Fertil. 1990;89:573–578
  31. Fukui Y, McGowan LT, James RW, Pugh PA, Tervit HR. Factors affecting the in vitro development to blastocysts of bovine oocytes matured and fertilized in vitro. J Reprod Fertil. 1991;92:125–131
  32. Takahashi Y, Kanagawa H. Effect of oxygen concentration in the gas atmosphere during in vitro insemination of bovine oocytes on the subsequent embryonic development in vitro. J Vet Med Sci. 1998;60:365–367
  33. Kaidi S, Donnay I, Van Langendonckt A, Dessy F, Massip A. Comparison of two co-culture systems to assess the survival of in vitro produced bovine blastocysts after vitrification. Anim Reprod Sci. 1998;52:39–50
  34. Lim JM, Reggio BC, Godke RA, Hansel W. Development of in-vitro–derived bovine embryos cultured in 5% CO2 in air or in 5% O2, 5% CO2 and 90% N2. Hum Reprod. 1999;14:458–464
  35. Rizos D, Ward F, Boland MP, Lonergan P. Effect of culture system on the yield and quality of bovine blastocysts as assessed by survival after vitrification. Theriogenology. 2001;56:1–16

 U.W. has nothing to disclose. A.E. has nothing to disclose. D.H. has nothing to disclose. S.N. has nothing to disclose.

PII: S0015-0282(08)00714-0

doi: 10.1016/j.fertnstert.2008.03.051

Fertility and Sterility
Volume 91, Issue 6 , Pages 2461-2465 , June 2009

Article Tools