Fertility and Sterility
Volume 91, Issue 6 , Pages 2611-2615, June 2009

Declining ovarian reserve adversely influences granulosa cell viability

  • Keri Greenseid, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology and Women's Health, Albert Einstein College of Medicine, Bronx, New York
    • Corresponding Author InformationReprint requests: Keri Greenseid, M.D., Department of Obstetrics and Gynecology and Women's Health, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Mazer 316, Bronx, NY 10461 (FAX: 718-430-8586).
    • ,
  • Sangita Jindal, Ph.D.

      Affiliations

    • Department of Obstetrics and Gynecology and Women's Health, Albert Einstein College of Medicine, Bronx, New York
    • ,
  • Athena Zapantis, B.S.

      Affiliations

    • Department of Obstetrics and Gynecology and Women's Health, Albert Einstein College of Medicine, Bronx, New York
    • ,
  • Michael Nihsen, M.S.

      Affiliations

    • Department of Obstetrics and Gynecology and Women's Health, Albert Einstein College of Medicine, Bronx, New York
    • ,
  • Joshua Hurwitz, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology and Women's Health, Albert Einstein College of Medicine, Bronx, New York
    • Reproductive Medicine Associates of Connecticut, Norwalk, Connecticut
    • ,
  • Lubna Pal, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology and Women's Health, Albert Einstein College of Medicine, Bronx, New York

Received 9 March 2007; received in revised form 20 March 2008; accepted 24 March 2008. published online 18 June 2008.

Objective

To identify if declining ovarian reserve adversely influences granulosa cell (GC) parameters in patients undergoing IVF.

Design

Prospective study.

Setting

Academic IVF Center.

Patient(s)

Twenty-four women undergoing IVF.

Intervention(s)

Mural and cumulus GCs were harvested (at time of egg retrieval).

Main Outcome Measure(s)

Influence of ovarian reserve, reflected by maximal basal FSH levels, on GC (cumulative, mural, and cumulus) counts and viability were investigated.

Result(s)

The FSH levels demonstrated an inverse correlation with cumulative and mural, but not cumulus, GC viability. Adverse influence of increasing FSH on cumulative GC viability was independent of patient's age. The GC counts were uninfluenced by ovarian reserve status. Neither GC count nor viability demonstrated any relationship with clinical pregnancy after IVF.

Conclusion(s)

We observed an adverse association between declining ovarian reserve and GC parameters. We believe these findings may partly explain the suboptimal reproductive performance in these women.

Key Words: Ovarian reserve, granulosa cell, mural, cumulus, follicle stimulating hormone

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 K.G. has nothing to disclose. S.J. has nothing to disclose. A.Z. has nothing to disclose. M.N. has nothing to disclose. J.H. has nothing to disclose. L.P. has nothing to disclose.

 Supported by a research grant from Ferring Pharmaceuticals, Parsipanny, New Jersey, and National Institutes of Health grant no. 5K12RR17672.

 Presented at the American Society of Reproductive Medicine 63rd annual meeting, Washington, DC, October 13–17, 2007.

PII: S0015-0282(08)00739-5

doi:10.1016/j.fertnstert.2008.03.065

Fertility and Sterility
Volume 91, Issue 6 , Pages 2611-2615, June 2009

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