Fertility and Sterility
Volume 92, Issue 1 , Pages 131-136 , July 2009

Artificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa

  • Edson Borges Jr., M.D., Ph.D.

      Affiliations

    • Fertility-Assisted Fertilization Center, São Paulo, Brazil
    • Sapientiae Institute, São Paulo, Brazil
    • Department of Gynecology and Obstetrics, Botucatu Medical School/UNESP Botucatu, São Paulo, Brazil
    • Corresponding Author InformationReprint requests: Edson Borges, Jr., M.D., Ph.D., Fertility–Assisted Fertilization Center and Sapientiae Institute Clinical, Av. Brigadeiro Luis Antonio 4545, 01401-002 São Paulo, Brazil (FAX: 55-11-38859858).
  • ,
  • Daniela Paes de Almeida Ferreira Braga, M.S.

      Affiliations

    • Fertility-Assisted Fertilization Center, São Paulo, Brazil
    • Sapientiae Institute, São Paulo, Brazil
  • ,
  • Tatiana Carvalho de Sousa Bonetti, M.S.

      Affiliations

    • Sapientiae Institute, São Paulo, Brazil
  • ,
  • Assumpto Iaconelli Jr., M.D.

      Affiliations

    • Fertility-Assisted Fertilization Center, São Paulo, Brazil
  • ,
  • José Gonçalves Franco Jr., M.D., Ph.D.

      Affiliations

    • Centre for Human Reproduction, Riberião preto, São Paulo, Brazil
    • Post Graduation Program, Department of Gynecology and Obstetrics, Botucatu Medical School/UNESP Botucatu, São Paulo, Brazil

Received 7 March 2008 ,Revised 21 April 2008 ,Accepted 21 April 2008.

References 

  1. Jarow JP, Espeland MA, Lipshultz LI. Evaluation of the azoospermic patient. J Urol. 1989;142:62–65
  2. Tournaye H, Verheyen G, Nagy P, Ubaldi F, Goossens A, Silber S, et al. Are there any predictive factors for successful testicular sperm recovery in azoospermic patients?. Hum Reprod. 1997;12:80–86
  3. Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;340:17–18
  4. Craft I, Bennett V, Nicholson N. Fertilising ability of testicular spermatozoa. Lancet. 1993;342:864
  5. Schoysman R, Vanderzwalmen P, Nijs M, Segal L, Segal-Bertin G, Geerts L, et al. Pregnancy after fertilisation with human testicular spermatozoa. Lancet. 1993;342:1237
  6. Dohle GR, Ramos L, Pieters MH, Braat DD, Weber RF. Surgical sperm retrieval and intracytoplasmic sperm injection as treatment of obstructive azoospermia. Hum Reprod. 1998;13:620–623
  7. Palermo GD, Schlegel PN, Hariprashad JJ, Ergun B, Mielnik A, Zaninovic N, et al. Fertilization and pregnancy outcome with intracytoplasmic sperm injection for azoospermic men. Hum Reprod. 1999;14:741–748
  8. Kupker W, al-Hasani S, Schulze W, Kuhnel W, Schill T, Felberbaum R, et al. Morphology in intracytoplasmic sperm injection: preliminary results. J Assist Reprod Genet. 1995;12:620–626
  9. Nagy ZP, Liu J, Joris H, Verheyen G, Tournaye H, Camus M, et al. The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum Reprod. 1995;10:1123–1129
  10. Sukcharoen N, Sithipravej T, Promviengchai S, Chinpilas V, Boonkasemsanti W. Sperm morphology evaluated by computer (IVOS) cannot predict the fertilization rate in vitro after intracytoplasmic sperm injection. Fertil Steril. 1998;69:564–568
  11. Tesarik J. Paternal effects on cell division in the human preimplantation embryo. Reprod Biomed Online. 2005;10:370–375
  12. Tesarik J, Mendoza-Tesarik R, Mendoza C. Sperm nuclear DNA damage: update on the mechanism, diagnosis and treatment. Reprod Biomed Online. 2006;12:715–721
  13. Rossi-Ferragut LM, Iaconelli A, Aoki T, Rocha CC, dos Santos DR, Pasqualotto FF, et al. Pronuclear and morphological features as a cumulative score to select embryos in ICSI (intracytoplasmic sperm injection) cycles according to sperm origin. J Assist Reprod Genet. 2003;20:1–7
  14. Pasqualotto FF, Rossi-Ferragut LM, Rocha CC, Iaconelli A, Borges E. Outcome of in vitro fertilization and intracytoplasmic injection of epididymal and testicular sperm obtained from patients with obstructive and nonobstructive azoospermia. J Urol. 2002;167:1753–1756
  15. Buffat C, Patrat C, Merlet F, Guibert J, Epelboin S, Thiounn N, et al. ICSI outcomes in obstructive azoospermia: influence of the origin of surgically retrieved spermatozoa and the cause of obstruction. Hum Reprod. 2006;21:1018–1024
  16. Ghazzawi IM, Sarraf MG, Taher MR, Khalifa FA. Comparison of the fertilizing capability of spermatozoa from ejaculates, epididymal aspirates and testicular biopsies using intracytoplasmic sperm injection. Hum Reprod. 1998;13:348–352
  17. Devroey P, Nagy P, Tournaye H, Liu J, Silber S, Van Steirteghem A. Outcome of intracytoplasmic sperm injection with testicular spermatozoa in obstructive and non-obstructive azoospermia. Hum Reprod. 1996;11:1015–1018
  18. Windt ML, Coetzee K, Kruger TF, Menkveld R, van der Merwe JP. Intracytoplasmic sperm injection with testicular spermatozoa in men with azoospermia. J Assist Reprod Genet. 2002;19:53–59
  19. Vernaeve V, Tournaye H, Osmanagaoglu K, Verheyen G, Van Steirteghem A, Devroey P. Intracytoplasmic sperm injection with testicular spermatozoa is less successful in men with nonobstructive azoospermia than in men with obstructive azoospermia. Fertil Steril. 2003;79:529–533
  20. De Croo I, Van der Elst J, Everaert K, De Sutter P, Dhont M. Fertilization, pregnancy and embryo implantation rates after ICSI in cases of obstructive and non-obstructive azoospermia. Hum Reprod. 2000;15:1383–1388
  21. Nasr-Esfahani MH, Razavi S, Mardani M, Shirazi R, Javanmardi S. Effects of failed oocyte activation and sperm protamine deficiency on fertilization post-ICSI. Reprod Biomed Online. 2007;14:422–429
  22. Stricker SA. Comparative biology of calcium signaling during fertilization and egg activation in animals. Developmental Biology. 1999;211:157–176
  23. Hafez ES, Goff L, Hafez B. Mammalian fertilization, IVF, ICSI: physiological/molecular parameters, clinical application. Arch Androl. 2004;50:69–88
  24. Carroll J, Jones KT, Whittingham DG. Ca2+ release and the development of Ca2+ release mechanisms during oocyte maturation: a prelude to fertilization. Rev Reprod. 1996;1:137–143
  25. Runft LL, Jaffe LA, Mehlmann LM. Egg activation at fertilization: where it all begins. Dev Biol. 2002;245:237–254
  26. Saunders CM, Larman MG, Parrington J, Cox LJ, Royse J, Blayney LM, et al. PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development. Development. 2002;129:3533–3544
  27. Katsuki T, Hara T, Ueda K, Tanaka J, Ohama K. Prediction of outcomes of assisted reproduction treatment using the calcium ionophore-induced acrosome reaction. Hum Reprod. 2005;20:469–475
  28. Nakagawa K, Yamano S, Moride N, Yamashita M, Yoshizawa M, Aono T. Effect of activation with Ca ionophore A23187 and puromycin on the development of human oocytes that failed to fertilize after intracytoplasmic sperm injection. Fertil Steril. 2001;76:148–152
  29. Chi HJ, Koo JJ, Song SJ, Lee JY, Chang SS. Successful fertilization and pregnancy after intracytoplasmic sperm injection and oocyte activation with calcium ionophore in a normozoospermic patient with extremely low fertilization rates in intracytoplasmic sperm injection cycles. Fertil Steril. 2004;82:475–477
  30. Kim ST, Cha YB, Park JM, Gye MC. Successful pregnancy and delivery from frozen-thawed embryos after intracytoplasmic sperm injection using round-headed spermatozoa and assisted oocyte activation in a globozoospermic patient with mosaic Down syndrome. Fertil Steril. 2001;75:445–447
  31. Murase Y, Araki Y, Mizuno S, Kawaguchi C, Naito M, Yoshizawa M, et al. Pregnancy following chemical activation of oocytes in a couple with repeated failure of fertilization using ICSI: case report. Hum Reprod. 2004;19:1604–1607
  32. Tesarik J, Mendoza C, Greco E. The activity (calcium oscillator?) responsible for human oocyte activation after injection with round spermatids is associated with spermatid nuclei. Fertil Steril. 2000;74:1245–1247
  33. Eldar-Geva T, Brooks B, Margalioth EJ, Zylber-Haran E, Gal M, Silber SJ. Successful pregnancy and delivery after calcium ionophore oocyte activation in a normozoospermic patient with previous repeated failed fertilization after intracytoplasmic sperm injection. Fertil Steril. 2003;79(Suppl 3):1656–1658
  34. Dale B, Fiorentino A, De Stefano R, Di Matteo L, De Simone ML, Wilding M, et al. Pregnancies after activated oocyte transfer: a new option for infertility treatment. Hum Reprod. 1999;14:1771–1772
  35. Heindryckx B, Van der Elst J, De Sutter P, Dhont M. Treatment option for sperm- or oocyte-related fertilization failure: assisted oocyte activation following diagnostic heterologous ICSI. Hum Reprod. 2005;20:2237–2241
  36. Wang E, Taylor RW, Pfeiffer DR. Mechanism and specificity of lanthanide series cation transport by ionophores A23187, 4-BrA23187, and ionomycin. Biophys J. 1998;75:1244–1254
  37. Nasr-Esfahani MH, Razavi S, Javdan Z, Tavalaee M. Artificial oocyte activation in severe teratozoospermia undergoing intracytoplasmic sperm injection. Fertil Steril. 2008;
  38. Hoshi K, Yanagida K, Yazawa H, Katayose H, Sato A. Intracytoplasmic sperm injection using immobilized or motile human spermatozoon. Fertil Steril. 1995;63:1241–1245
  39. Rybouchkin AV, Van der Straeten F, Quatacker J, De Sutter P, Dhont M. Fertilization and pregnancy after assisted oocyte activation and intracytoplasmic sperm injection in a case of round-headed sperm associated with deficient oocyte activation capacity. Fertil Steril. 1997;68:1144–1147
  40. Tesarik J, Sousa M. More than 90% fertilization rates after intracytoplasmic sperm injection and artificial induction of oocyte activation with calcium ionophore. Fertil Steril. 1995;63:343–349
  41. Yanagida K, Morozumi K, Katayose H, Hayashi S, Sato A. Successful pregnancy after ICSI with strontium oocyte activation in low rates of fertilization. Reprod Biomed Online. 2006;13:801–806
  42. Ahmady A, Michael E. Successful pregnancy and delivery following intracytoplasmic injection of frozen-thawed nonviable testicular sperm and oocyte activation with calcium ionophore. J Androl. 2007;28:13–14
  43. Check JH, Levito MC, Summers-Chase D, Marmar J, Barci H. A comparison of the efficacy of intracytoplasmic sperm injection (ICSI) using ejaculated sperm selected by high magnification versus ICSI with testicular sperm both followed by oocyte activation with calcium ionophore. Clin Exp Obstet Gynecol. 2007;34:111–112
  44. Saunders CM, Swann K, Lai FA. PLCzeta, a sperm-specific PLC and its potential role in fertilization. Biochem Soc Symp. 2007;74:23–36
  45. Swann K, Larman MG, Saunders CM, Lai FA. The cytosolic sperm factor that triggers Ca2+ oscillations and egg activation in mammals is a novel phospholipase C: PLCzeta. Reproduction. 2004;127:431–439
  46. Dozortsev D, Qian C, Ermilov A, Rybouchkin A, De Sutter P, Dhont M. Sperm-associated oocyte-activating factor is released from the spermatozoon within 30 minutes after injection as a result of the sperm–oocyte interaction. Hum Reprod. 1997;12:2792–2796
  47. Yanagimachi R. Mammalian fertilization. In:  Knobil E,  Neill J editor. The physiology of reproduction. 2nd ed.. New York: Raven Press; 1994;p. 189–317
  48. Dadoune JP. The nuclear status of human sperm cells. Micron. 1995;26:323–345
  49. Paffoni A, Brevini TA, Somigliana E, Restelli L, Gandolfi F, Ragni G. In vitro development of human oocytes after parthenogenetic activation or intracytoplasmic sperm injection. Fertil Steril. 2007;87:77–82
  50. Tesarik J, Rienzi L, Ubaldi F, Mendoza C, Greco E. Use of a modified intracytoplasmic sperm injection technique to overcome sperm-borne and oocyte-borne oocyte activation failures. Fertil Steril. 2002;78:619–624
  51. Manipalviratn S, Ahnonkitpanit V, Numchaisrika P, Chompurat D, Pansatha J, Suwajanakorn S. Results of direct current electrical activation of failed-to-fertilize oocytes after intracytoplasmic sperm injection. J Reprod Med. 2006;51:493–499
  52. Bos-Mikich A, Whittingham DG, Jones KT. Meiotic and mitotic Ca2+ oscillations affect cell composition in resulting blastocysts. Dev Biol. 1997;182:172–179
  53. Ozil JP, Huneau D. Activation of rabbit oocytes: the impact of the Ca2+ signal regime on development. Development. 2001;128:917–928
  54. Ozil JP, Banrezes B, Toth S, Pan H, Schultz RM. Ca2+ oscillatory pattern in fertilized mouse eggs affects gene expression and development to term. Dev Biol. 2006;300:534–544
  55. Rout UK, Krawetz SA, Armant DR. Ethanol-induced intracellular calcium mobilization rapidly alters gene expression in the mouse blastocyst. Cell Calcium. 1997;22:463–474

 E.B. has nothing to disclose. D.P.F.B. has nothing to disclose. T.C.B. has nothing to disclose. A.I. has nothing to disclose. J.G.F. has nothing to disclose.

PII: S0015-0282(08)00961-8

doi: 10.1016/j.fertnstert.2008.04.046

Fertility and Sterility
Volume 92, Issue 1 , Pages 131-136 , July 2009