« Previous
Next »
Fertility and Sterility
Volume 92, Issue 2
, Pages 819-827
, August 2009
Evaluation of chemiluminescence and flow cytometry as tools in assessing production of hydrogen peroxide and superoxide anion in human spermatozoa
References
- . The role of free radicals and antioxidants in reproduction. Current Opin Obstet Gynecol. 2006;18:325–332
- . Sperm function tests and fertility. Int J Androl. 2006;29:69–75discussion, 105–8
- . Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril. 2003;79:829–843
- . What an andrologist/urologist should know about free radicals and why. Urology. 2006;67:2–8
- . Oxidative stress, DNA damage and the Y chromosome. Reproduction (Camb). 2001;122:497–506
- . Impact of reactive oxygen species on spermatozoa: a balancing act between beneficial and detrimental effects. Hum Reprod. 1995;10(Suppl 1):15–21
- Leukocytospermia is associated with increased reactive oxygen species production by human spermatozoa. Fertil Steril. 2002;78:1215–1224
- Increased DNA damage in sperm from leukocytospermic semen samples as determined by the sperm chromatin structure assay. Fertil Steril. 2002;78:319–329
- Increased sperm nuclear DNA damage in normozoospermic infertile men: a prospective study. Fertil Steril. 2002;78:313–318
- . Effect of leukocytospermia on sperm DNA integrity: a negative effect in abnormal semen samples. J Androl. 2002;23:717–723
- . Study of mitochondrial membrane potential, reactive oxygen species, DNA fragmentation and cell viability by flow cytometry in human sperm. Hum Reprod. 2002;17:1257–1265
- . Relationship between seminal white blood cell counts and oxidative stress in men treated at an infertility clinic. J Androl. 2001;22:575–583
- . Origins and consequences of DNA damage in male germ cells. Reprod Biomed Online. 2007;14:727–733
- . Chemiluminescence technique for measuring reactive oxygen species. Reprod Biomed Online. 2004;9:466–468
- Quality control of reactive oxygen species measurement by luminol-dependent chemiluminescence assay. J Androl. 2001;22:568–574
- . Shedding light on chemiluminescence: the application of chemiluminescence in diagnostic andrology. J Androl. 2004;25:455–465
- Development of normal reference values for seminal reactive oxygen species and their correlation with leukocytes and semen parameters in a fertile population. J Androl. 2007;28:613–620
- . A multicenter study evaluating the flow cytometric-based kit for semen analysis. Fertil Steril. 2005;83:1034–1038
- . Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry. J Anim Sci. 2006;84:2089–2100
- A new multiparameter flow cytometric method for human semen analysis. Hum Reprod. 2007;22:485–494
- . Recent advances in fluorescent probes for the detection of reactive oxygen species. Anal Bioanal Chem. 2006;386:532–543
- . Editorial commentary on “Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: potential implications in intracellular fluorescence detection of superoxide” by H. Zhao, et al. Free Rad Biol Med. 2003;34:1357–1358
- Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: potential implications in intracellular fluorescence detection of superoxide. Free Rad Biol Med. 2003;34:1359–1368
- . The oxidation of 2′,7′-dichlorofluorescin to reactive oxygen species: a self-fulfilling prophesy?. Free Rad Biol Med. 2006;40:968–975
- . WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed.. Cambridge: Cambridge University Press; 1999;
- Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility. Hum Reprod. 2001;16:1912–1921
- In: Team RDC editors. R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing; 2006;
- . Evaluation of flow cytometric methods to measure human sperm concentration. J Androl. 2001;22:558–567
- . Evaluation of assays for the measurement of bovine neutrophil reactive oxygen species. Vet Immunol Immunopathol. 2007;115:107–125
- . Measurement of intracellular fluorescence of human monocytes relative to oxidative metabolism. J Leuk Biol. 1988;43:304–310
- . Definitive evidence for the nonmitochondrial production of superoxide anion by human spermatozoa. J Clin Endocrinol Metab. 2006;91:1968–1975
- . Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin N Am. 2002;29:817–827
- . Reactive oxygen species and human spermatozoa: analysis of the cellular mechanisms involved in luminol- and lucigenin-dependent chemiluminescence. J Cell Physiol. 1992;151:466–477
- . Reactive oxygen species and human spermatozoa: physiology and pathology. Int J Androl. 1997;20:61–69
- . Oxidative stress and male infertility: from research bench to clinical practice. J Androl. 2002;23:737–752
- . Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. J Androl. 2004;25:5–18
- . Constant decline in sperm concentration in infertile males in an urban population: experience over 18 years. Fertil Steril. 2005;84:1657–1661
- . How reliable are results from the semen analysis?. Fertil Steril. 2004;82:41–44
- Quality control of reactive oxygen species measurement by luminol-dependent chemiluminescence assay. J Androl. 2001;22:568–574
- . Impact of cryopreservation and reactive oxygen species on DNA integrity, lipid peroxidation, and functional parameters in ram sperm. Mol Reprod Devel. 2007;74:878–892
R.M. has nothing to disclose. R.S. has nothing to disclose. J.L. has nothing to disclose. N.A. has nothing to disclose. A.A. has nothing to disclose.
Presented at the 63rd Annual Meeting of the American Society of Reproductive Medicine, which was held in Washington, D.C., on October 13–19, 2007.
Supported by a research grant from the Research Programs Committee, Cleveland Clinic (RPC no. 07549).
PII: S0015-0282(08)01246-6
doi: 10.1016/j.fertnstert.2008.05.087
© 2009 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
« Previous
Next »
Fertility and Sterility
Volume 92, Issue 2
, Pages 819-827
, August 2009

