Fertility and Sterility
Volume 92, Issue 3 , Pages 897-903 , September 2009

Selective microsurgical removal of a pronucleus from tripronuclear human oocytes to restore diploidy: disregarded but valuable?

  • Bernd E. Rosenbusch, Ph.D.

      Affiliations

    • Corresponding Author InformationReprint requests: Bernd E. Rosenbusch, Ph.D., Department of Gynecology and Obstetrics, University of Ulm, Prittwitzstrasse 43, D-89075 Ulm, Germany (FAX: ++49-731-500-58664).

Received 31 March 2008 ,Revised 12 June 2008 ,Accepted 14 July 2008.

References 

  1. Pieters MHEC, Dumoulin JCM, Ignoul-Vanvuchelen RCM, Bras M, Evers JLH, Geraedts JPM. Triploidy after in vitro fertilization: cytogenetic analysis of human zygotes and embryos. J Assist Reprod Genet. 1992;9:68–76
  2. Porter R, Han T, Tucker MJ, Graham J, Liebermann J, Sills ES. Estimation of second polar body retention rate after conventional insemination and intracytoplasmic sperm injection: in vitro observations from more than 5000 human oocytes. J Assist Reprod Genet. 2003;20:371–376
  3. Macas E, Imthurn B, Roselli M, Keller PJ. The chromosomal complements of multipronuclear human zygotes resulting from intracytoplasmic sperm injection. Hum Reprod. 1996;11:2496–2501
  4. Rosenbusch B. Mechanisms giving rise to triploid zygotes during assisted reproduction. Fertil Steril. 2008;90:49–55
  5. Malter HE, Cohen J. Embryonic development after microsurgical repair of polyspermic human zygotes. Fertil Steril. 1989;52:373–380
  6. Veeck LL. An atlas of human gametes and conceptuses. Casterton Hall, Carnforth: Parthenon Publishing; 1999;
  7. Palermo G, Munné S, Cohen J. The human zygote inherits its mitotic potential from the male gamete. Hum Reprod. 1994;9:1220–1225
  8. Chida S. Micromanipulation on oocytes for the defect of monospermic fertilization: intracytoplasmic sperm injection and extra male pronuclear extraction. Jpn J Fertil Steril. 1996;41:100–105(in Japanese)
  9. Rawlins RG, Binor Z, Radwanska E, Dmowski WP. Microsurgical enucleation of tripronuclear human zygotes. Fertil Steril. 1988;50:266–272
  10. Gordon JW, Grunfeld L, Garrisi GJ, Navot D, Laufer N. Successful microsurgical removal of a pronucleus from tripronuclear human zygotes. Fertil Steril. 1989;52:367–372
  11. Tang Y-X, Munné S, Reing A, Schattman G, Grifo J, Cohen J. The parental origin of the distal pronucleus in dispermic human zygotes. Zygote. 1994;2:79–85
  12. Ivakhnenko V, Cieslak J, Verlinsky Y. A microsurgical technique for enucleation of multipronuclear human zygotes. Hum Reprod. 2000;15:911–916
  13. Kattera S, Chen C. Normal birth after microsurgical enucleation of tripronuclear human zygotes: case report. Hum Reprod. 2003;18:1319–1322
  14. Escribá M-J, Martín J, Rubio C, Valbuena D, Remohí J, Pellicer A, et al. Heteroparental blastocyst production from microsurgically corrected tripronucleated human embryos. Fertil Steril. 2006;86:1601–1607
  15. McGrath J, Solter D. Nuclear transplantation in the mouse embryo by microsurgery and cell fusion. Science. 1983;220:1300–1302
  16. Palermo GD, Colombero LT, Rosenwaks Z. The human sperm centrosome is responsible for normal syngamy and early embryonic development. Rev Reprod. 1997;2:19–27
  17. Kola I, Trounson A, Dawson G, Rogers P. Tripronuclear human oocytes: altered cleavage patterns and subsequent karyotypic analysis of embryos. Biol Reprod. 1987;37:395–401
  18. Wiker S, Malter H, Wright G, Cohen J. Recognition of paternal pronuclei in human zygotes. J In Vitro Fertil Embryo Transfer. 1990;7:33–37
  19. Van Blerkom J, Bell H, Henry G. The occurrence, recognition and developmental fate of pseudo-multipronuclear eggs after in-vitro fertilization of human oocytes. Hum Reprod. 1987;2:217–225
  20. Egozcue S, Blanco J, Vidal F, Egozcue J. Diploid sperm and the origin of triploidy. Hum Reprod. 2002;17:5–7
  21. Rosenbusch B, Schneider M, Kreienberg R, Brucker C. Cytogenetic analysis of human zygotes displaying three pronuclei and one polar body after intracytoplasmic sperm injection. Hum Reprod. 2001;16:2362–2367
  22. Rosenbusch B, Schneider M. Hypotriploid tripronuclear oocytes with two polar bodies obtained after ICSI: is irregular chromatid segregation involved?. Hum Reprod. 2000;15:1876–1877
  23. Flaherty SP, Payne D, Matthews CD. Fertilization failures and abnormal fertilization after intracytoplasmic sperm injection. Hum Reprod. 1998;13(Suppl 1):155–164
  24. Sadowy S, Tomkin G, Munné S, Ferrara-Congedo T, Cohen J. Impaired development of zygotes with uneven pronuclear size. Zygote. 1998;6:137–141
  25. Manor D, Drugan A, Stein D, Pillar M, Itskovitz-Eldor J. Unequal pronuclear size—a powerful predictor of embryonic chromosome anomalies. J Assist Reprod Genet. 1999;16:385–389
  26. Feng YL, Gordon JW. Birth of normal mice after removal of the supernumerary male pronucleus from polyspermic zygotes. Hum Reprod. 1996;11:341–344
  27. Payne D, Flaherty SP, Barry MF, Matthews CD. Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography. Hum Reprod. 1997;12:532–541
  28. Feenan K, Herbert M. Can “abnormally” fertilized zygotes give rise to viable embryos?. Hum Fertil (Camb). 2006;9:157–169

 B.E.R. has nothing to disclose.

PII: S0015-0282(08)03263-9

doi: 10.1016/j.fertnstert.2008.07.1740

Fertility and Sterility
Volume 92, Issue 3 , Pages 897-903 , September 2009