Stimulation of endometrium embryo transfer can improve implantation and pregnancy rates for patients undergoing assisted reproductive technology for the first time with a high-grade blastocyst

Objective

To evaluate whether stimulation of endometrium embryo transfer (SEET) can improve implantation rate and pregnancy rate (PR) for patients undergoing assisted reproductive technology (ART) for the first time by injecting embryo culture supernatant into the uterus before blastocyst transfer (BT).

Design

Randomized, controlled trial.

Setting

Private in vitro fertilization clinic.

Patient(s)

Forty-eight women in the BT group, 48 women in the stimulation group who had culture medium injected into the uterus before BT, and 48 women in the SEET group.

Intervention(s)

Injection of embryo culture supernatant and injection of culture medium.

Main Outcome Measure(s)

Implantation rates and PRs.

Result(s)

Odds ratios of successful implantation rate for stimulation and SEET in patients with high-grade blastocysts, having BT as reference, were 2.58 and 6.46 without adjustment, and 5.91 and 9.20 after adjusting for basal FSH levels and period of infertility. Odds ratios of clinical pregnancies were 2.47 and 4.32 without adjustment, and 4.46 and 5.10 with adjustment, respectively. In groups with low-grade blastocysts, such tendencies were not observed.

Conclusion(s)

The SEET may be an effective method for increasing implantation rate and PR for first-time ART patients who have a high-grade blastocyst.

Key Words: SEET, blastocyst transfer, embryo culture supernatant, cross-talk